scholarly journals Super-resolution imaging technique based on a LCoS display: Increase of CCD resolution limit

2013 ◽  
Vol 46 (3) ◽  
pp. 223-230 ◽  
Author(s):  
M. Sohail
Keyword(s):  
Imaging Technique ◽  
Super Resolution ◽  
Resolution Limit ◽  
Resolution Imaging

scholarly journals On super-resolution imaging as a multiparameter estimation problem

2017 ◽  
Vol 15 (08) ◽  
pp. 1740005 ◽  
Author(s):  
Andrzej Chrostowski ◽  
Rafał Demkowicz-Dobrzański ◽  
Marcin Jarzyna ◽  
Konrad Banaszek
Keyword(s):  
Phase Shift ◽  
Light Source ◽  
Imaging Technique ◽  
Super Resolution ◽  
Estimation Problem ◽  
Joint Estimation ◽  
Spatial Extent ◽  
Resolution Imaging

We consider the problem of characterizing the spatial extent of a composite light source using the super-resolution imaging technique based on mode demultiplexing when the centroid of the source is not known precisely. We show that the essential features of this problem can be mapped onto a simple qubit model for joint estimation of a phase shift and a dephasing strength.

scholarly journals Demonstration of nanoimprinted hyperlens array for high-throughput sub-diffraction imaging

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Minseop Byun ◽  
Dasol Lee ◽  
Minkyung Kim ◽  
Yangdoo Kim ◽  
Kwan Kim ◽  
...  
Keyword(s):  
Real Time ◽  
Large Scale ◽  
Medical Science ◽  
Super Resolution ◽  
Fabrication Process ◽  
Far Field ◽  
Resolution Limit ◽  
Imaging Science ◽  
Diffraction Imaging ◽  
Resolution Imaging

Abstract Overcoming the resolution limit of conventional optics is regarded as the most important issue in optical imaging science and technology. Although hyperlenses, super-resolution imaging devices based on highly anisotropic dispersion relations that allow the access of high-wavevector components, have recently achieved far-field sub-diffraction imaging in real-time, the previously demonstrated devices have suffered from the extreme difficulties of both the fabrication process and the non-artificial objects placement. This results in restrictions on the practical applications of the hyperlens devices. While implementing large-scale hyperlens arrays in conventional microscopy is desirable to solve such issues, it has not been feasible to fabricate such large-scale hyperlens array with the previously used nanofabrication methods. Here, we suggest a scalable and reliable fabrication process of a large-scale hyperlens device based on direct pattern transfer techniques. We fabricate a 5 cm × 5 cm size hyperlenses array and experimentally demonstrate that it can resolve sub-diffraction features down to 160 nm under 410 nm wavelength visible light. The array-based hyperlens device will provide a simple solution for much more practical far-field and real-time super-resolution imaging which can be widely used in optics, biology, medical science, nanotechnology and other closely related interdisciplinary fields.

scholarly journals Super-resolution imaging of a 2.5 kb non-repetitive DNA in situ in the nuclear genome using molecular beacon probes

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Yanxiang Ni ◽  
Bo Cao ◽  
Tszshan Ma ◽  
Gang Niu ◽  
Yingdong Huo ◽  
...  
Keyword(s):  
Repetitive Dna ◽  
Molecular Beacon ◽  
Genomic Sequence ◽  
Single Cells ◽  
Nuclear Genome ◽  
Super Resolution ◽  
Resolution Limit ◽  
Fish Method ◽  
Resolution Imaging

High-resolution visualization of short non-repetitive DNA in situ in the nuclear genome is essential for studying looping interactions and chromatin organization in single cells. Recent advances in fluorescence in situ hybridization (FISH) using Oligopaint probes have enabled super-resolution imaging of genomic domains with a resolution limit of 4.9 kb. To target shorter elements, we developed a simple FISH method that uses molecular beacon (MB) probes to facilitate the probe-target binding, while minimizing non-specific fluorescence. We used three-dimensional stochastic optical reconstruction microscopy (3D-STORM) with optimized imaging conditions to efficiently distinguish sparsely distributed Alexa-647 from background cellular autofluorescence. Utilizing 3D-STORM and only 29–34 individual MB probes, we observed 3D fine-scale nanostructures of 2.5 kb integrated or endogenous unique DNA in situ in human or mouse genome, respectively. We demonstrated our MB-based FISH method was capable of visualizing the so far shortest non-repetitive genomic sequence in 3D at super-resolution.

scholarly journals From 2D to 3D super-resolution imaging through glass microspheres -INVITED

2020 ◽  
Vol 238 ◽  
pp. 06002
Author(s):  
Stephane Perrin ◽  
Sylvain Lecler ◽  
Paul Montgomery
Keyword(s):  
3D Reconstruction ◽  
Imaging Technique ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Label Free ◽  
Magnification Factor ◽  
Glass Microspheres ◽  
Full Field ◽  
Resolution Imaging ◽  
2D To 3D

Microsphere-assisted microscopy is a new imaging technique which allows the diffraction limit to be overcome using transparent microspheres. It makes it possible to reach a resolution of up to 100 nm in air while being label-free and full-field. An overview of the imaging technique is presented showing the influence of the photonic jet on the image nature and the unconventional behaviour of the magnification factor. Moreover, interferometry through microspheres is demonstrated for the 3D reconstruction of nanoelements.

scholarly journals Recent Progress in the Correlative Structured Illumination Microscopy

Chemosensors ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 364
Author(s):  
Meiting Wang ◽  
Jiajie Chen ◽  
Lei Wang ◽  
Xiaomin Zheng ◽  
Jie Zhou ◽  
...  
Keyword(s):  
High Frequency ◽  
Optical Transmission ◽  
Imaging Technique ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Optical Diffraction ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Recent Developments ◽  
Resolution Imaging

The super-resolution imaging technique of structured illumination microscopy (SIM) enables the mixing of high-frequency information into the optical transmission domain via light-source modulation, thus breaking the optical diffraction limit. Correlative SIM, which combines other techniques with SIM, offers more versatility or higher imaging resolution than traditional SIM. In this review, we first briefly introduce the imaging mechanism and development trends of conventional SIM. Then, the principles and recent developments of correlative SIM techniques are reviewed. Finally, the future development directions of SIM and its correlative microscopies are presented.

Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein

Nano Letters ◽  
2019 ◽  
Vol 20 (4) ◽  
pp. 2197-2208 ◽  
Author(s):  
Mingshu Zhang ◽  
Zhifei Fu ◽  
Changqing Li ◽  
Anyuan Liu ◽  
Dingming Peng ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Imaging Technique ◽  
Super Resolution ◽  
Immediate Early ◽  
Resolution Imaging

scholarly journals High-Resolution Optical Imaging of Zebrafish Larval Ribbon Synapse Protein RIBEYE, RIM2, and CaV 1.4 by Stimulation Emission Depletion Microscopy

2012 ◽  
Vol 18 (4) ◽  
pp. 745-752 ◽  
Author(s):  
Caixia Lv ◽  
Travis J. Gould ◽  
Joerg Bewersdorf ◽  
David Zenisek
Keyword(s):  
Super Resolution ◽  
Photoreceptor Cells ◽  
Stimulated Emission ◽  
Resolution Limit ◽  
Synaptic Ribbon ◽  
Small Spot ◽  
Ribbon Structure ◽  
Resolution Imaging ◽  
Plexiform Layers ◽  
Fish Retina

AbstractThe synaptic ribbon is a unique presynaptic structure with an intricate morphology in photoreceptors. Because of the resolution limit in conventional fluorescence microscopy, investigating ribbon protein locations has been challenging, especially in the early development stages of model animals. Here, we used stimulated emission depletion microscopy, a super-resolution imaging technique, to look at retina sections in 4 days post-fertilization (dpf) zebrafish. We observed that in photoreceptor cells, RIBEYE and RIM2 are expressed along the synaptic ribbon, with RIM2 consistently located inside of the horseshoe-shaped synaptic ribbon structure with RIBEYE located on the outside. The L-type calcium channel subunit, CACNA1F, exhibited small spot-like staining beneath the RIM2 and RIBEYE structures. Using morpholino antisense oligonucleotides to knock down RIBEYE expression, we observed fewer and shorter ribbons in the photoreceptor outer plexiform layers of 4 dpf fish retina as well as a reduction in RIM2 expression. The clustering of CACNA1F in these blind fish was no longer observed, but instead showed a diffuse expression in the photoreceptor terminal.

Advantages of microsphere-assisted super-resolution imaging technique over solid immersion lens and confocal microscopies

2014 ◽  
Vol 104 (6) ◽  
pp. 061117 ◽  
Author(s):  
Arash Darafsheh ◽  
Nicholaos I. Limberopoulos ◽  
John S. Derov ◽  
Dennis E. Walker ◽  
Vasily N. Astratov
Keyword(s):  
Imaging Technique ◽  
Super Resolution ◽  
Solid Immersion Lens ◽  
Resolution Imaging
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