scholarly journals Bystander effect formation in the sprout root meristem in condition of irradiated and non-irradiated seeds joint growing

2018 ◽  
Vol 22 ◽  
pp. 28-33
Author(s):  
O. Yu. German
Keyword(s):  
Statistical Analysis ◽  
Gamma Radiation ◽  
Mitotic Activity ◽  
Small Dose ◽  
Bystander Effect ◽  
Root Meristem ◽  
Activity Level ◽  
High Dose ◽  
Aqueous Environment ◽  
Cytogenetic Methods

Aim. To study the possibility of bystander effect inducing in the joint germination in a common aqueous environment of irradiated and non-irradiated seeds. Methods. Cytogenetic methods including mitotic activity level estimation, accounting for the number of nucleoli, and methods for statistical analysis of the data. Results. The effect of radiation stimulation with a dose of 40 Gy was dependent on the variety, and the effect of gamma radiation at a dose of 200 Gy reduced the level of mitotic activity in comparison with the control in both varieties. Mitotic indices increase in the meristem of non-irradiated seeds while growing with irradiated one. Conclusions. The bystander effect is formed on the organismic level under condition of growing of irradiated and non-irradiated seeds in a common aquatic environment and it is ma-nifested as an increase in the level of meristem cells proliferation and in the intensification of synthetic processes. The addition of irradiated with a high dose of gamma radiation seeds to the non-irradiated seeds induces an increase in mitotic activity largely than the addition of seeds irradiated with a relatively small dose. Keywords: bystander effect, radiation stimulation, mitotic activity, nucleoli.

scholarly journals Direct and Bystander Effect on Cervix Cancer Cells (SiHa) Exposed to High Dose-Rate Gamma Radiation Sourced from Ir 192 Used in Brachytherapy

2015 ◽  
Vol 1 (1) ◽  
pp. 007-013 ◽  
Author(s):  
Kulkarni Megha Anilkumar ◽  
Syed Basheerudeen Safa Abdul ◽  
Xavier Sidonia Vallas ◽  
Appaswamy Murugan ◽  
Kuppusamy Thayalan ◽  
...  
Keyword(s):  
Gamma Radiation ◽  
Dose Rate ◽  
Cancer Cells ◽  
Bystander Effect ◽  
High Dose Rate ◽  
Cervix Cancer ◽  
High Dose

scholarly journals Induction of bystander effect in root meristem of soybean seedlings after γ-irradiation

2019 ◽  
Keyword(s):  
Ionizing Radiation ◽  
Mitotic Activity ◽  
Aquatic Environment ◽  
Bystander Effect ◽  
Root Meristem ◽  
Direct Action ◽  
Genetically Modified ◽  
Γ Irradiation ◽  
Soybean Seedlings ◽  
The Common

The bystander effect refers to the non-target effects of ionizing radiation and it is the occurrence of radiation damages in the cells that have not been exposed to direct action of ionizing radiation. The aim of the study is to investigate the possibility of forming the “bystander effect” during the germination of irradiated and intact seeds of some soybean Glycine max (L.) Mer. varieties in the common aquatic environment. The soybean seeds of the Raiduga and Sprytna varieties (obtained by selection) and the genetically modified Apollo variety had been exposed to γ-radiation at a dose of 40 Gy. The mitotic activity of the seedlings root meristem cells of irradiated (IR) and intact (IN) seeds, as well as intact seeds, which were germinated in the same aqueous medium together with irradiated (INIR), was analyzed. The similar levels of mitotic activity were observed in seedlings of breeding varieties in “IN” variant, while cells in genetically modified variety divided more intensively. Radiation exposure in a dose of 40 Gy increased the level of mitotic activity in all varieties. The values of the mitotic indices increased 2 times in the varieties of Raiduga and Sprytna. The increase in Apollo variety was less significant, but this may be due to a high level of mitotic activity in the control variant. Irradiation also contributed to the appearance of chromosomal aberrations: fragments and bridges. The increase in mitotic activity in the meristem of the studied varieties to the mentioned levels may indicate the presence of a pool of meristem cells that can accelerate the passage of phases of the mitotic cycle under extreme conditions. Mitotic activity increased in the “INIR” variant in all investigated varieties. The largest excess over the “IN” was in Sprytna, a little less in the variety Raiduga. The proliferative activity in the Apollo variety meristem remained almost unchanged. Thus, the work shows the possibility of a “bystander effect” forming under the condition of joint germination of irradiated and intact seeds in the common aquatic environment. The intensity of “bystander effect” formation depends on the genotype and start mitotic potential.

scholarly journals Study of Mitotic Activity and Chromosomal Behaviour in Root Meristem of Allium cepa L. Treated with Magnesium Sulphate

2009 ◽  
Vol 15 ◽  
pp. 83-88 ◽  
Author(s):  
Prabhakar Bhatta ◽  
S. R. Sakya
Keyword(s):  
Key Words ◽  
Mitotic Activity ◽  
Allium Cepa ◽  
Biological System ◽  
Magnesium Sulphate ◽  
Root Meristem ◽  
Chromosomal Abnormalities ◽  
Inhibitory Effect ◽  
Duration Of Treatment ◽  
Chromosomal Behaviour

The mitotic activity and chromosomal behaviour was observed in four different concentrations of magnesium sulphate at four different duration of treatment using Allium cepa as a biological system. Mito-inhibitory effect of the compound was observed in higher concentration. The compound was capable of producing various chromosomal abnormalities indicating the cytostatic and clastogenic properties of magnesium sulphate. Key words: Allium cepa, magnesium sulphate, mitotic index, phase indices.doi: 10.3126/eco.v15i0.1947 ECOPRINT 15: 83-88, 2008

scholarly journals Bystander Effect between Zebrafish Embryos in Vivo Induced by High-Dose X-rays

2013 ◽  
Vol 47 (12) ◽  
pp. 6368-6376 ◽  
Author(s):  
V. W. Y. Choi ◽  
C. Y. P. Ng ◽  
A. Kobayashi ◽  
T. Konishi ◽  
N. Suya ◽  
...  
Keyword(s):  
Bystander Effect ◽  
High Dose ◽  
Zebrafish Embryos ◽  
X Rays

scholarly journals High Dose Gamma Radiation Selectively Reduces GABAA-slow Inhibition

Cureus ◽  
2017 ◽  
Author(s):  
Beza A Dagne ◽  
Melis K Sunay ◽  
Noëlie S Cayla ◽  
Yi-Bing Ouyang ◽  
Susan J Knox ◽  
...  
Keyword(s):  
Gamma Radiation ◽  
High Dose

scholarly journals Prevalence of Malnutrition (Overweight and Underweight) in Children Aged 10-13 Years in Schools in Manipal and Utopia, Karnataka

2020 ◽  
Vol 10 (1) ◽  
pp. 94
Author(s):  
Fatemeh Jahani
Keyword(s):  
Physical Activity ◽  
Statistical Analysis ◽  
Activity Level ◽  
Normal Height ◽  
Major Health Problem ◽  
Major Health ◽  
Severe Stunting ◽  
Increased Risk ◽  
Child Eating ◽  
Height For Age

Background. Malnutrition is a major health problem in India. It mostly affects children of school going age with serious health problems putting them at an increased risk for developing chronic diseases later in life. This study aims to study the prevalence of malnutrition (overweight and underweight) in children aged 10-13 years in schools in Manipal and Utopia, Karnataka. Materials and Methods. In this study, a total 400 students were studied. Their anthropometric measurements like height, weight, BMI and MUAC, dietary pattern, physical activity level, and child eating behavior. Questionnaire was used to note the student’s information. The data collected was subjected to statistical analysis using SPSS version 21, Diet Cal, and Anthroplus1.0.4. Results. It was observed that in the childhood category 10-13 years there were 222 (55.5%) male and 178(44.5%) female. According to the BMI for age 53.4% of them falls under the normal category; 22.3% falls under the mild wasting category; About 18.3% falls under the obese category and 8% falls under the moderate wasting category. There is no students falling under the severe wasting and overweight category according to the BAZ classification. When the height for age was done for all the children, about 36.3% falls under normal height; About 35.5% falls under mild stunting; 19.3% falls under the moderate stunting; 8.3% falls under the severe stunting category; and 1% falls under tall category according to the HAZ classification. Conclusions. In conclusion, mild stunting and wasting was observed among children. There are no students falling under the severe wasting and overweight category according to the BAZ classification.

Effect of Gamma Radiation and Oregano Essential Oil on Murein and ATP Concentration of Escherichia coli O157:H7

2005 ◽  
Vol 68 (12) ◽  
pp. 2571-2579 ◽  
Author(s):  
STÉPHANE CAILLET ◽  
FRANÇOIS SHARECK ◽  
MONIQUE LACROIX
Keyword(s):  
Escherichia Coli ◽  
Essential Oil ◽  
Gamma Radiation ◽  
Cell Damage ◽  
Wall Structure ◽  
High Dose ◽  
Escherichia Coli O157 ◽  
Oregano Essential Oil ◽  
Intracellular Atp ◽  
Oregano Oil

This study was carried out to evaluate the effects of gamma radiation alone or in combination with oregano essential oil on the murein composition of Escherichia coli O157:H7 and on the intracellular and extracellular concentrations of ATP. The bacterial strain was treated with three radiation doses: 0.4 kGy to induce cell damage, 1.1 kGy to obtain a viable but nonculturable state, and 1.3 kGy to cause cell death. Oregano essential oil was used at 0.006 and 0.025% (wt/vol), which is the MIC. All treatments had a significant effect (P ≤ 0.05) on the murein composition, although some muropeptides did not seem to be affected by the treatment. Each treatment had a different effect on the relative percentage and number of muropeptides. There was a significant correlation (P ≤ 0.05) between the decrease in intracellular ATP and the increase in extracellular ATP following treatment of the cells with oregano oil. The reduction of intracellular ATP was even more important when oregano oil was combined with irradiation, but irradiation alone at a high dose (≤1.1 kGy) significantly decreased (P ≤ 0.05) the internal ATP without affecting the external ATP. Transmission electron microscopic examination revealed that oregano oil and irradiation have an effect on cell wall structure.

scholarly journals Seasonal variations in the incidence of auroral radio absorption events at very high latitude, and the influence of the magnetotail

2007 ◽  
Vol 25 (3) ◽  
pp. 711-720 ◽  
Author(s):  
J. K. Hargreaves
Keyword(s):  
Statistical Analysis ◽  
Seasonal Variations ◽  
Polar Region ◽  
Winter Season ◽  
Magnetic Activity ◽  
Time Of Day ◽  
Activity Level ◽  
The Sun ◽  
South Pole ◽  
Very High

Abstract. A statistical analysis has been made of the incidence of auroral radio absorption events at South Pole, and of its dependence on basic geophysical parameters such as season, time of day, and magnetic activity level. It is found that at low and moderate levels of activity the incidence of events in the winter season is at least twice that in the summer. However, at high activity no events at all occurred during the local summer night, which appears to be explicable as the effect of the magnetotail and the consequent distortion of the magnetosphere when the southern polar region is tilted strongly towards the Sun. Previous results from even higher latitudes show the effect in an even more exaggerated form, in that both the day and night periods of absorption activity exhibit strong seasonal variations.

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