scholarly journals Study on carbohydrate specificity of hemolytic lectin from death-cap mushroom (Amanita phalloides (Vaill. Fr.) Secr)

2005 ◽  
Vol 21 (4) ◽  
pp. 319-325 ◽  
Author(s):  
V. O. Antonyuk
Keyword(s):  
Amanita Phalloides ◽  
Carbohydrate Specificity

scholarly journals Reinvestigation of Carbohydrate Specificity of EBCA-1 Monoclonal Antibody Used for the Detection of Candida Mannan

2021 ◽  
Vol 7 (7) ◽  
pp. 504
Author(s):  
Vadim B. Krylov ◽  
Arsenii S. Solovev ◽  
Ilya A. Puchkin ◽  
Dmitry V. Yashunsky ◽  
Anna V. Antonets ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Invasive Candidiasis ◽  
Carbohydrate Specificity ◽  
Coated Plate ◽  
Sera Samples ◽  
Immune Assay

Monoclonal antibody EBCA-1 is used in the sandwich immune assay for the detection of circulating Candida mannan in blood sera samples for the diagnosis of invasive candidiasis. To reinvestigate carbohydrate specificity of EBCA-1, a panel of biotinylated oligosaccharides structurally related to distinct fragments of Candida mannan were loaded onto a streptavidin-coated plate to form a glycoarray. Its use demonstrated that EBCA-1 recognizes the trisaccharide β-Man-(1→2)-α-Man-(1→2)-α-Man and not homo-α-(1→2)-linked pentamannoside, as was reported previously.

Intravenous rifampicin use in the management of amanita phalloides toxicity

2021 ◽  
pp. 1-4
Author(s):  
Rona Zuker-Herman ◽  
Roger Tong ◽  
Anselm Wong
Keyword(s):  
Amanita Phalloides

scholarly journals Acute Liver Failure Caused byAmanita phalloidesPoisoning

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Luca Santi ◽  
Caterina Maggioli ◽  
Marianna Mastroroberto ◽  
Manuel Tufoni ◽  
Lucia Napoli ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Liver Failure ◽  
Acute Liver Failure ◽  
Rna Polymerase Ii ◽  
Gastrointestinal Symptoms ◽  
Amanita Phalloides ◽  
Prognostic Indicators ◽  
Mushroom Poisoning ◽  
Lag Phase ◽  
Kidney Involvement

Mushroom poisoning is a relatively rare cause of acute liver failure (ALF). The present paper analyzes the pathogenesis, clinical features, prognostic indicators, and therapeutic strategies of ALF secondary to ingestion ofAmanita phalloides, which represents the most common and deadly cause of mushroom poisoning. Liver damage fromAmanita phalloidesis related to the amanitins, powerful toxins that inhibit RNA polymerase II resulting in a deficient protein synthesis and cell necrosis. After an asymptomatic lag phase, the clinical picture is characterized by gastrointestinal symptoms, followed by the liver and kidney involvement. Amatoxin poisoning may progress into ALF and eventually death if liver transplantation is not performed. The mortality rate afterAmanita phalloidespoisoning ranges from 10 to 20%. The management of amatoxin poisoning consists of preliminary medical care, supportive measures, detoxification therapies, and orthotopic liver transplantation. The clinical efficacy of any modality of treatment is difficult to demonstrate since randomized, controlled clinical trials have not been reported. The use of extracorporeal liver assist devices as well as auxiliary liver transplantation may represent additional therapeutic options.

Carbohydrate specificity and opsonin dependency of the interaction between human senescent red cells and autologous monocytes

1986 ◽  
Vol 3 (4) ◽  
pp. 379-389 ◽  
Author(s):  
L Gattegno ◽  
M J Prigent ◽  
L Saffar ◽  
D Bladier ◽  
J Vaysse ◽  
...  
Keyword(s):  
Red Cells ◽  
Carbohydrate Specificity

scholarly journals A breakthrough on Amanita phalloides poisoning: an effective antidotal effect by polymyxin B

2015 ◽  
Vol 89 (12) ◽  
pp. 2305-2323 ◽  
Author(s):  
Juliana Garcia ◽  
Vera Marisa Costa ◽  
Alexandra T. P. Carvalho ◽  
Ricardo Silvestre ◽  
José Alberto Duarte ◽  
...  
Keyword(s):  
Polymyxin B ◽  
Amanita Phalloides

Tailoring GalNAcα1-3Galβ-specific lectins from a multi-specific fungal galectin: dramatic change of carbohydrate specificity by a single amino-acid substitution

2013 ◽  
Vol 453 (2) ◽  
pp. 261-270 ◽  
Author(s):  
Dan Hu ◽  
Hiroaki Tateno ◽  
Takashi Sato ◽  
Hisashi Narimatsu ◽  
Jun Hirabayashi
Keyword(s):  
Dissociation Constants ◽  
Multiple Roles ◽  
Site Directed Mutagenesis ◽  
Saturation Mutagenesis ◽  
Single Amino Acid ◽  
Dramatic Improvement ◽  
Carbohydrate Specificity ◽  
Cell Staining ◽  
Group A

Galectins exhibit multiple roles through recognition of diverse structures of β-galactosides. However, this broad specificity often hinders their practical use as probes. In the present study we report a dramatic improvement in the carbohydrate specificity of a multi-specific fungal galectin from the mushroom Agrocybe cylindricea, which binds not only to simple β-galactosides, but also to their derivatives. Site-directed mutagenesis targeting five residues involved in β-galactose binding revealed that replacement of Asn46 with alanine (N46A) increased the binding to GalNAcα1-3Galβ-containing glycans, while eliminating binding to all other β-galactosides, as shown by glycoconjugate microarray analysis. Quantitative analysis by frontal affinity chromatography showed that the mutant N46A had enhanced affinity towards blood group A tetraose (type 2), A hexaose (type 1) and Forssman pentasaccharide with dissociation constants of 5.0×10−6 M, 3.8×10−6 M and 1.0×10−5 M respectively. Surprisingly, all the other mutants generated by saturation mutagenesis of Asn46 exhibited essentially the same specificity as N46A. Moreover, alanine substitution for Pro45, which forms the cis-conformation upon β-galactose binding, exhibited the same specificity as N46A. From a practical viewpoint, the derived N46A mutant proved to be unique as a specific probe to detect GalNAcα1-3Galβ-containing glycans by methods such as flow cytometry, cell staining and lectin microarray.

scholarly journals Amanita phalloides poisoning and treatment with silibinin in the Australian Capital Territory and New South Wales

2013 ◽  
Vol 198 (1) ◽  
pp. 43-47 ◽  
Author(s):  
Darren M Roberts ◽  
Michael J Hall ◽  
Morna M Falkland ◽  
Simone I Strasser ◽  
Nick A Buckley
Keyword(s):  
New South ◽  
New South Wales ◽  
Amanita Phalloides ◽  
Australian Capital Territory ◽  
Australian Capital ◽  
South Wales
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