scholarly journals Reinvestigation of Carbohydrate Specificity of EBCA-1 Monoclonal Antibody Used for the Detection of Candida Mannan

2021 ◽  
Vol 7 (7) ◽  
pp. 504
Author(s):  
Vadim B. Krylov ◽  
Arsenii S. Solovev ◽  
Ilya A. Puchkin ◽  
Dmitry V. Yashunsky ◽  
Anna V. Antonets ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Invasive Candidiasis ◽  
Carbohydrate Specificity ◽  
Coated Plate ◽  
Sera Samples ◽  
Immune Assay

Monoclonal antibody EBCA-1 is used in the sandwich immune assay for the detection of circulating Candida mannan in blood sera samples for the diagnosis of invasive candidiasis. To reinvestigate carbohydrate specificity of EBCA-1, a panel of biotinylated oligosaccharides structurally related to distinct fragments of Candida mannan were loaded onto a streptavidin-coated plate to form a glycoarray. Its use demonstrated that EBCA-1 recognizes the trisaccharide β-Man-(1→2)-α-Man-(1→2)-α-Man and not homo-α-(1→2)-linked pentamannoside, as was reported previously.

scholarly journals Human Monoclonal Antibody-Based Therapy in the Treatment of Invasive Candidiasis

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Francesca Bugli ◽  
Margherita Cacaci ◽  
Cecilia Martini ◽  
Riccardo Torelli ◽  
Brunella Posteraro ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Invasive Candidiasis ◽  
Human Monoclonal Antibody ◽  
Heat Shock Protein 90 ◽  
Biological Properties ◽  
Cell Mediated Immunity ◽  
Antibody Molecule ◽  
Life Threatening ◽  
Mucosal Candidiasis ◽  
New Formulation

Invasive candidiasis (IC) represents the leading fungal infection of humans causing life-threatening disease in immunosuppressed and neutropenic individuals including also the intensive care unit patients. Despite progress in recent years in drugs development for the treatment of IC, morbidity and mortality rates still remain very high. Historically, cell-mediated immunity and innate immunity are considered to be the most important lines of defense against candidiasis. Nevertheless recent evidence demonstrates that antibodies with defined specificities could act with different degrees showing protection against systemic and mucosal candidiasis. Mycograb is a human recombinant monoclonal antibody against heat shock protein 90 (Hsp90) that was revealed to have synergy when combined with fluconazole, caspofungin, and amphotericin B against a broad spectrum ofCandidaspecies. Furthermore, recent studies have established an important role for Hsp90 in mediatingCandidaresistance to echinocandins, giving to this antibody molecule even more attractive biological properties. In response to the failure of marketing authorization by the CHMP (Committee for Medicinal Products for Human Use) a new formulation of Mycograb, named Mycograb C28Y variant, with an amino acid substitution was developed in recent years. First data on Mycograb C28Y variant indicate that this monoclonal antibody lacked efficacy in a murine candidiasis model.

scholarly journals A Fungicidal Monoclonal Antibody Protects against Murine Invasive Candidiasis

2006 ◽  
Vol 74 (5) ◽  
pp. 3042-3045 ◽  
Author(s):  
María J. Sevilla ◽  
Beatriz Robledo ◽  
Aitor Rementeria ◽  
María D. Moragues ◽  
José Pontón
Keyword(s):  
Monoclonal Antibody ◽  
Candida Albicans ◽  
Invasive Candidiasis

ABSTRACT Mice infected by Candida albicans and treated with monoclonal antibody C7 survived longer than saline-treated animals. A prozone-like effect was observed. The in vitro candidacidal activity of macrophages was strongly enhanced when C. albicans was opsonized by C7 and complete murine serum was present.

scholarly journals Reinvestigation of carbohydrate specificity of EB-A2 monoclonal antibody used in the immune detection of Aspergillus fumigatus galactomannan

Heliyon ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. e01173 ◽  
Author(s):  
Vadim B. Krylov ◽  
Arsenii S. Solovev ◽  
Dmitry A. Argunov ◽  
Jean-Paul Latgé ◽  
Nikolay E. Nifantiev
Keyword(s):  
Monoclonal Antibody ◽  
Aspergillus Fumigatus ◽  
Carbohydrate Specificity ◽  
Immune Detection

scholarly journals Use of Monoclonal Antibody in Diagnosis of Candidiasis Caused by Candida albicans: Detection of Circulating Aspartyl Proteinase Antigen

1999 ◽  
Vol 6 (6) ◽  
pp. 924-929 ◽  
Author(s):  
Byoung-Kuk Na ◽  
Chul-Yong Song
Keyword(s):  
Monoclonal Antibody ◽  
Candida Albicans ◽  
Invasive Candidiasis ◽  
Healthy Individuals ◽  
Serum Samples ◽  
Capture Elisa ◽  
Inhibition Elisa ◽  
Antigen Capture Elisa ◽  
Antigen Capture ◽  
Secreted Aspartyl Proteinase

ABSTRACT To develop a serological diagnosis of invasive candidiasis based on detection of circulating secreted aspartyl proteinase (SAP) antigen ofCandida albicans, three different enzyme-linked immunosorbent assays (ELISAs) were compared. The first was a standard ELISA to detect anti-SAP antibodies, and the others were an antigen capture ELISA and an inhibition ELISA to detect circulating SAP antigen with monoclonal antibody (MAb) CAP1, which is highly specific for SAP. These tests were applied to 33 serum samples retrospectively selected from 33 patients with mycologically and/or serologically proven invasive candidiasis caused by C. albicans. Serum samples from 12 patients with aspergillosis and serum samples from 13 healthy individuals were also included. The sensitivities and specificities were 69.7 and 76.0% for the standard ELISA and 93.9 and 92.0% for the antigen capture ELISA, respectively. However, these values reached 93.9 and 96.0%, respectively, for the inhibition ELISA. Serum samples from 31 of 33 patients had detectable SAP antigen, with concentrations ranging from 6.3 to 19.0 ng/ml. These results indicate that the inhibition ELISA with MAb CAP1 is effective in detection of circulating SAP antigen and that this assay may be useful for diagnosis and treatment monitoring of invasive candidiasis.

Novel Carbohydrate Specificity of Monoclonal Antibody 91.9H Prepared against Human Colonic Sulfomucin: Recognition of Sulfo-Lewis a Structure

1998 ◽  
Vol 253 (2) ◽  
pp. 374-381 ◽  
Author(s):  
Hitomi Tsuiji ◽  
Joe C. Hong ◽  
Young S. Kim ◽  
Yuzuru Ikehara ◽  
Hisashi Narimatsu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Carbohydrate Specificity ◽  
Lewis A

A web-like network of type vi collagen in human skin is revealed by immuno-electron microscopy

1988 ◽  
Vol 46 ◽  
pp. 382-383
Author(s):  
Douglas R. Keene ◽  
Robert W. Glanville ◽  
Eva Engvall
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Human Skin ◽  
Basement Membranes ◽  
Primary Antibody ◽  
Fat Cells ◽  
Secondary Antibody ◽  
Type Vi Collagen ◽  
Dermal Matrix ◽  
Immuno Electron Microscopy

A mouse monoclonal antibody (5C6) prepared against human type VI collagen (1) has been used in this study to immunolocalize type VI collagen in human skin. The enbloc method used involves exposing whole tissue pieces to primary antibody and 5 nm gold conjugated secondary antibody before fixation, and has been described in detail elsewhere (2).Biopsies were taken from individuals ranging in age from neonate to 65 years old. By immuno-electron microscopy, type VI collagen is found to be distributed as a fine branching network closely associated with (but not attached to) banded collagen fibrils containing types I and III collagen (Fig. 1). It appears to enwrap fibers, to weave between individual fibrils within a fiber, and to span the distance separating fibers, creating a “web-like network” which entraps fibers within deep papillary and reticular dermal layers (Fig. 2). Relative to that in the dermal matrix, the concentration of type VI collagen is higher around endothelial basement membranes limiting the outer boundaries of nerves, capillaries, and fat cells (Fig. 3).

High-voltage electron microscopy of subretinal scar formation

1992 ◽  
Vol 50 (1) ◽  
pp. 486-487
Author(s):  
G.E. Korte ◽  
M. Marko ◽  
G. Hageman
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Retinal Pigment Epithelium ◽  
Glial Cells ◽  
High Voltage ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Sodium Iodate ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron

Sodium iodate iv. damages the retinal pigment epithelium (RPE) in rabbits. Where RPE does not regenerate (e.g., 1,2) Muller glial cells (MC) forma subretinal scar that replaces RPE. The MC response was studied by HVEM in 3D computer reconstructions of serial thick sections, made using the STEREC0N program (3), and the HVEM at the NYS Dept. of Health in Albany, NY. Tissue was processed for HVEM or immunofluorescence localization of a monoclonal antibody recognizing MG microvilli (4).

Identification of the pulmonary syndrome hantavirus by direct and colloidal gold immune Electron Microscopy

1994 ◽  
Vol 52 ◽  
pp. 274-275
Author(s):  
C. D. Humphrey ◽  
C.S. Goldsmith ◽  
L. Elliott ◽  
S.R. Zaki
Keyword(s):  
Electron Microscopy ◽  
United States ◽  
Monoclonal Antibody ◽  
Colloidal Gold ◽  
Phosphotungstic Acid ◽  
Uranyl Acetate ◽  
Hantavirus Pulmonary Syndrome ◽  
Deer Mice ◽  
Immune Electron Microscopy ◽  
Negative Stain

An outbreak of unexplained acute pulmonary syndrome with high fatality was recognized in the spring of 1993 in the southwestern United States. The cause of the illness was quickly identified serologically and genetically as a hantavirus and the disease was named hantavirus pulmonary syndrome (HPS). Recently, the virus was isolated from deer mice which had been trapped near the homes of HPS patients, and cultivated in Vero E6 cells. We identified the cultivated virus by negative-stain direct and colloidal gold immune electron microscopy (EM).Virus was extracted, clarified, and concentrated from unfixed and 0.25% glutaraldehyde fixed supernatant fluids of infected Vero E6 cells by a procedure described previously. Concentrated virus suspensions tested by direct EM were applied to glow-discharge treated formvar-carbon filmed grids, blotted, and stained with 0.5% uranyl acetate (UA) or with 2% phosphotungstic acid (PTA) pH 6.5. Virus suspensions for immune colloidal gold identification were adsorbed similarly to filmed grids but incubated for 1 hr on drops of 1:50 diluted monoclonal antibody to Prospect Hill virus nucleoprotein or with 1:50 diluted sera from HPS virus infected deer mice.

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