scholarly journals Interaction of cyanine dyes with nucleic acids. Meso-methylsubstituted trimethincyanines, as possible probes for fluorescent nucleic acid detection

2001 ◽  
Vol 17 (5) ◽  
pp. 448-454 ◽  
Author(s):  
S. S. Lukashov ◽  
I. E. Makovenko ◽  
M. Yu. Losytskyy ◽  
Yu. L. Slominskii ◽  
S. M. Yarmoluk
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Cyanine Dyes ◽  
Nucleic Acid Detection

scholarly journals A nuclease protection ELISA assay for colorimetric and electrochemical detection of nucleic acids

2019 ◽  
Vol 11 (8) ◽  
pp. 1027-1034 ◽  
Author(s):  
Jessica E. Filer ◽  
Robert B. Channon ◽  
Charles S. Henry ◽  
Brian J. Geiss
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Electrochemical Detection ◽  
Nucleic Acid Detection ◽  
Elisa Assay

The NP-ELISA combines traditional nuclease protection with optical and electrochemical enzymatic readout for nucleic acid detection.

Preparation of monomethine cyanine dyes for nucleic acid detection

1998 ◽  
Vol 37 (3) ◽  
pp. 205-211 ◽  
Author(s):  
Todor G Deligeorgiev ◽  
Daphinka A Zaneva ◽  
Seok Hong Kim ◽  
Ram W Sabnis
Keyword(s):  
Nucleic Acid ◽  
Cyanine Dyes ◽  
Nucleic Acid Detection

Recent advance in exonuclease Ⅲ-assisted target signal amplification strategy for nucleic acid detection

2021 ◽  
Author(s):  
hongyu liu ◽  
Yuhao You ◽  
Youzhuo Zhu ◽  
Heng Zheng
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Molecular Diagnostics ◽  
Mutation Analysis ◽  
Signal Amplification ◽  
Nucleic Acid Detection ◽  
Target Signal ◽  
Forensic Investigations ◽  
Exo Iii ◽  
Amplification Strategy

Detection of nucleic acids have become significantly important in molecular diagnostics, genetics therapy, mutation analysis, forensic investigations and biomedical development, and so on. In recent years, exonuclease Ⅲ (Exo III)...

Synthesis of novel monomeric and homodimeric cyanine dyes with thioacetyl substituents for nucleic acid detection

2007 ◽  
Vol 72 (1) ◽  
pp. 28-32 ◽  
Author(s):  
Todor Deligeorgiev ◽  
Nikolai Gadjev ◽  
Aleksey Vasilev ◽  
Karl-Heinz Drexhage ◽  
S.M. Yarmoluk
Keyword(s):  
Nucleic Acid ◽  
Cyanine Dyes ◽  
Nucleic Acid Detection

A catalytic DNA circuit-programmed and enzyme-powered autonomous DNA machine for nucleic acid detection

The Analyst ◽  
2019 ◽  
Vol 144 (20) ◽  
pp. 5923-5927 ◽  
Author(s):  
Shuang Liu ◽  
Chen Xin ◽  
Xiaoxiao Yu ◽  
Zhenbo Ding ◽  
Shufeng Liu
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Nucleic Acid Detection ◽  
One Step ◽  
Catalytic Dna ◽  
Dna Machine

A catalytic DNA circuit-programmed and enzyme-powered autonomous DNA machine was proposed for one-step, isothermal and dual-level amplified detection of nucleic acids.

NAIL: Nucleic Acid detection using Isotachophoresis and Loop-mediated isothermal amplification

Lab on a Chip ◽  
2015 ◽  
Vol 15 (7) ◽  
pp. 1697-1707 ◽  
Author(s):  
Mark D. Borysiak ◽  
Kevin W. Kimura ◽  
Jonathan D. Posner
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Mobile Phone ◽  
Isothermal Amplification ◽  
Nucleic Acid Detection ◽  
Complex Matrices ◽  
Loop Mediated Isothermal Amplification

The NAIL device integrates isotachophoresis and loop-mediated isothermal amplification (LAMP) with mobile phone detection to extract, amplify, and detect nucleic acids from complex matrices in less than one hour.

Intercalating Cyanine Dyes for Nucleic Acid Detection

2009 ◽  
Vol 2 (1) ◽  
pp. 1-26 ◽  
Author(s):  
Todor Deligeorgiev ◽  
Stefka Kaloyanova ◽  
Juan Vaquero
Keyword(s):  
Nucleic Acid ◽  
Cyanine Dyes ◽  
Nucleic Acid Detection

Study of dielectrophoresis effect of dipolar molecules in electric field of nanopore and development of medical diagnostic nanopore sensor

2015 ◽  
Author(s):  
◽  
Kai Tian
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Early Stage ◽  
Medical Diagnostics ◽  
Nucleic Acid Detection ◽  
Detection Accuracy ◽  
University Of Missouri ◽  
Complex Samples ◽  
Medical Diagnostic ◽  
Polycationic Peptide

[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] The nanopore sensor can detect cancer-derived nucleic acid biomarkers such as microRNAs (miRNAs), providing a noninvasive tool potentially useful in medical diagnostics. However, the nanopore-based detection of these biomarkers remains confounded by the presence of numerous other nucleic acid species found in biofluid extracts. Their nonspecific interactions with the nanopore inevitably contaminate the target signals, reducing the detection accuracy. Here we report a novel method that utilizes a polycationic peptide-PNA probe as the carrier for selective nucleic acid detection in the nucleic acids mixture. The cationic probe hybridized with DNA or RNA forms a dipole complex, which can be captured by the pore using a voltage polarity that is opposite the polarity used to capture negatively charged nucleic acids. As a result, non-target species are driven away from the pore opening, and the target sequences can be detected accurately without interference. In addition, we demonstrate that the PNA probe enables to accurately discriminate single-nucleotide difference. Moreover, molecule dynamic simulation is applied to expose the mechanism. Combined with experimental and calculating data, we construct a model to demonstrate that it is universal for all kinds of nucleic acid targets. In sum, this highly sensitive and selective nano-dielectrophoresis approach can be applied to the detection of clinically relevant nucleic acid fragments in complex samples and fulfills the diagnostic of diseases in early stage.

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