scholarly journals DNA probe construction with the use of silver salt. 3. Dosed modification of two-stranded probe and additional colouring of hybrids

1996 ◽  
Vol 12 (5) ◽  
pp. 29-33
Author(s):  
A. V. Shugalii ◽  
M. V. Lichina
Keyword(s):  
Author(s):  
Gary Bassell ◽  
Robert H. Singer

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.


2006 ◽  
Vol 89 (12) ◽  
pp. 121501 ◽  
Author(s):  
Guangliang Chen ◽  
Wenjun Zhao ◽  
Shihua Chen ◽  
Mingyan Zhou ◽  
Wenran Feng ◽  
...  

2021 ◽  
pp. 1-11
Author(s):  
Haixia Wang ◽  
Xuejiao Wang ◽  
Mingyuan Zhao ◽  
Chao Shi ◽  
Cuiping Ma

RSC Advances ◽  
2021 ◽  
Vol 11 (35) ◽  
pp. 21796-21804
Author(s):  
Seo Won Cho ◽  
Hyun Jeong Lim ◽  
Beelee Chua ◽  
Ahjeong Son

Principle of an ssDNA paired aptasensor where extra dye binding sites are created to enhance its fluorescence response.


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