scholarly journals Species Differentiation of DNA using Dot-Blots with Biotinylated or 32P-Labeled Genomic DNA Probe

1987 ◽  
Vol 28 (3-4) ◽  
pp. 451-453
Author(s):  
P. Dybdahl Thomsen ◽  
K. Christensen
Keyword(s):  
Genomic Dna ◽  
Dna Probe ◽  
Species Differentiation

scholarly journals Back Cover: A Mycoplasma Genomic DNA Probe using Gated Nanoporous Anodic Alumina (ChemPlusChem 3/2017)

ChemPlusChem ◽  
2017 ◽  
Vol 82 (3) ◽  
pp. 467-467
Author(s):  
Luís Pla ◽  
Elisabet Xifré-Pérez ◽  
Àngela Ribes ◽  
Elena Aznar ◽  
M. Dolores Marcos ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Anodic Alumina ◽  
Dna Probe ◽  
Back Cover ◽  
Nanoporous Anodic Alumina

A species-specific oligonucleotide DNA probe for the identification of Meloidogyne incognita

Parasitology ◽  
1991 ◽  
Vol 103 (2) ◽  
pp. 315-319 ◽  
Author(s):  
M. R. Chacon ◽  
R. M. E. Parkhouse ◽  
M. P. Robinson ◽  
P. R. Burrows ◽  
T. Garate
Keyword(s):  
Dna Sequences ◽  
Diagnostic Tool ◽  
Meloidogyne Incognita ◽  
Genomic Dna ◽  
Genomic Library ◽  
Dna Probe ◽  
Equal Length ◽  
Race 1 ◽  
Species Specific

A genomic library of Meloidogyne incognita Race 1 has been prepared in the bacteriophage λgt10 and screened for specific DNA sequences by hybridization with radio-isotope labelled total genomic DNA from a number of Meloidogyne species. One clone isolated (MR1#15), although not totally species specific, clearly showed preferential hybridization to M. incognita. Following subcloning and sequencing of the 255 bp insert, four stretches of the sequence corresponding to oligonucleotides of approximately equal length (~60 bp) were synthesized and examined for specificity. One of them, MR1#15.2, showed the necessary specificity to be used as a diagnostic tool.

scholarly journals Identification of Vibrio halioticoli by colony hybridization with non-radioisotope labeled genomic DNA probe

2002 ◽  
Vol 68 (1) ◽  
pp. 227-229 ◽  
Author(s):  
REIJI TANAKA ◽  
MASASHI OOTSUBO ◽  
TOMOO SAWABE ◽  
KENICHI TAJIMA ◽  
JOHAN VANDENBERGHE ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Dna Probe ◽  
Colony Hybridization

DNA fingerprinting analysis ofPetromyces alliaceus(AspergillussectionFlavi)

2005 ◽  
Vol 51 (12) ◽  
pp. 1039-1044 ◽  
Author(s):  
Cesaria E McAlpin ◽  
Donald T Wicklow
Keyword(s):  
Dna Fingerprinting ◽  
Aspergillus Flavus ◽  
Genomic Dna ◽  
Genotypic Diversity ◽  
Dna Probe ◽  
Dna Fingerprint ◽  
Dna Fingerprints ◽  
Close Relationship ◽  
Aspergillus Alliaceus ◽  
Aspergillus Section

The objective of this study was to evaluate the ability of the Aspergillus flavus pAF28 DNA probe to produce DNA fingerprints for distinguishing among genotypes of Petromyces alliaceus (Aspergillus section Flavi), a fungus considered responsible for the ochratoxin A contamination that is occasionally observed in California fig orchards. P. alliaceus (14 isolates), Petromyces albertensis (one isolate), and seven species of Aspergillus section Circumdati (14 isolates) were analyzed by DNA fingerprinting using a repetitive sequence DNA probe pAF28 derived from A. flavus. The presence of hybridization bands with the DNA probe and with the P. alliaceus or P. albertensis genomic DNA indicates a close relationship between A. flavus and P. alliaceus. Twelve distinct DNA fingerprint groups or genotypes were identified among the 15 isolates of Petromyces. Conspecificity of P. alliaceus and P. albertensis is suggested based on DNA fingerprints. Species belonging to Aspergillus section Circumdati hybridized only slightly at the 7.0-kb region with the repetitive DNA probe, unlike the highly polymorphic hybridization patterns obtained from P. alliaceus and A. flavus, suggesting very little homology of the probe to Aspergillus section Circum dati genomic DNA. The pAF28 DNA probe offers a tool for typing and monitoring specific P. alliaceus clonal populations and for estimating the genotypic diversity of P. alliaceus in orchards, vineyards, or crop fields.Key words: Aspergillus alliaceus, Circumdati, DNA probe, genotypic diversity, hybridization patterns, ochratoxin, Southern blot.

Total genomic DNA probe to detect Giardia lamblia

The Lancet ◽  
1990 ◽  
Vol 336 (8709) ◽  
pp. 257 ◽  
Author(s):  
D.J.M. Lewis ◽  
E.L. Green ◽  
F. Ashall
Keyword(s):  
Giardia Lamblia ◽  
Genomic Dna ◽  
Dna Probe

Isolation and characterization ofAzospirillum lipoferumlocus that complementsRhizobium meliloti dctAanddctBmutations

1996 ◽  
Vol 42 (5) ◽  
pp. 503-506 ◽  
Author(s):  
A. K. Tripathi ◽  
B. M. Mishra
Keyword(s):  
Structural Gene ◽  
Genomic Dna ◽  
Genomic Library ◽  
Rhizobium Meliloti ◽  
Dna Probe ◽  
Azospirillum Lipoferum ◽  
Gene Complementation ◽  
Dicarboxylate Transport ◽  
Isolation And Characterization

A DNA probe containing the structural gene for dicarboxylate transport (dctA) of Rhizobium meliloti hybridized strongly with the fragments of Azospirillum lipoferum genomic DNA. A genomic library of A. lipoferum was screened for the dctA gene by complementation of a dctA mutant of Rhizobium meliloti. A recombinant cosmid, p37D, capable of restoring growth of the dctA mutant on dicarboxylates was isolated and found to hybridize to the dctA probe. The ability of p37D to complement the dctB mutant of R. meloliti indicated that dctA and dctB genes in A. lipoferum may be organized adjacent to each other.Key words: Azospirillum lipoferum, dicarboxylate transport gene, complementation cloning.

Whole Genomic DNA Probe for Detection of Porphyromonas endodontalis

2000 ◽  
Vol 26 (4) ◽  
pp. 217-220 ◽  
Author(s):  
R NISSAN ◽  
S MAKKAR ◽  
M SELA ◽  
R STEVENS
Keyword(s):  
Genomic Dna ◽  
Dna Probe

scholarly journals A Mycoplasma Genomic DNA Probe using Gated Nanoporous Anodic Alumina

ChemPlusChem ◽  
2017 ◽  
Vol 82 (3) ◽  
pp. 337-341 ◽  
Author(s):  
Luís Pla ◽  
Elisabet Xifré-Pérez ◽  
Àngela Ribes ◽  
Elena Aznar ◽  
M. Dolores Marcos ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Anodic Alumina ◽  
Dna Probe ◽  
Nanoporous Anodic Alumina
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