Histologic Evaluations of Xenotransplanted Rabbit Knees by In Vitro-Propagated Human Amniotic Epithelial Cells: A Preclinical Study

2020 ◽  
Vol 18 (3) ◽  
pp. 375-381
Author(s):  
F. Dina Elessawi ◽  
Radwan K. Nashwa ◽  
Waleed A. Nemr
Keyword(s):  
Epithelial Cells ◽  
Preclinical Study ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells

scholarly journals Hyaluronic acid ameliorates the proliferative ability of human amniotic epithelial cells through activation of TGF-β/BMP signaling

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10104
Author(s):  
Ya-Bing Tian ◽  
Nuo-Xin Wang ◽  
Yan Xu ◽  
Chang-Yin Yu ◽  
Ru-Ming Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hyaluronic Acid ◽  
Epithelial Cells ◽  
Bmp Signaling ◽  
Inflammatory Factors ◽  
Proliferative Effect ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells ◽  
Proliferative Ability

Human amniotic epithelial cells (hAECs) are a useful and noncontroversial source of stem cells for cell therapy and regenerative medicine, but their limited proliferative ability hinders the acquisition of adequate quantities of cells for clinical use due to not expressing telomerase in hAECs. Our previous study showed that hyaluronic acid (HA), an important component of the extracellular matrix, promoted the proliferation of human amniotic mesenchymal stem cells. Herein, we hypothesize that HA might improve the proliferative capability of hAECs. In the present study, the role of HA on the proliferation of human amniotic epithelial cells (hAECs) in vitro was investigated for the first time. HA at molecular weight of 300 kDa showed an obvious pro-proliferation effect on hAECs. Furthermore, HA not only kept phenotypic characteristics and differentiation capabilities of hAECs, but significantly promoted the secretion of the anti-inflammatory factors such as IL-10 and TGF-β1, and the expression of stem cell pluripotent factors such as Oct4 and Nanog. Analysis of PCR microarray data and RT-qPCR validation showed that TGF-β/BMP signaling was activated in the presence of HA. Further study showed that SB431542, an inhibitor of the TGF-β/BMP signaling, significantly suppressed the mRNA expression of TGFBR3, BMP4, BMP7, BMPR1B, SMAD3, SMAD4, and the pro-proliferative effect of HA on hAECs. These data suggest that HA is a safe and effective enhancer for in vitro expansion of hAECs, whose regulatory mechanism involves the TGF-β/BMP signaling.

scholarly journals In vitro differentiation of human amniotic epithelial cells into insulin-producing 3D spheroids

2015 ◽  
Vol 28 (3) ◽  
pp. 390-402 ◽  
Author(s):  
Bernard Okere ◽  
Francesco Alviano ◽  
Roberta Costa ◽  
Daniela Quaglino ◽  
Francesca Ricci ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
In Vitro Differentiation ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells ◽  
3D Spheroids

scholarly journals Hepatocyte-Like Cells Derived from Human Amniotic Epithelial Cells Can Be Encapsulated Without Loss of Viability or Function In Vitro

2014 ◽  
Vol 23 (8) ◽  
pp. 866-876 ◽  
Author(s):  
Vijesh Vaghjiani ◽  
Vijayaganapathy Vaithilingam ◽  
Indah Saraswati ◽  
Adnan Sali ◽  
Padma Murthi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells

Phenotypic shift of human amniotic epithelial cells in culture is associated with reduced osteogenic differentiation in vitro

Cytotherapy ◽  
2008 ◽  
Vol 10 (7) ◽  
pp. 743-752 ◽  
Author(s):  
G. Stadler ◽  
S. Hennerbichler ◽  
A. Lindenmair ◽  
A. Peterbauer ◽  
K. Hofer ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Osteogenic Differentiation ◽  
Cells In Culture ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells ◽  
Phenotypic Shift

scholarly journals Prematurity negatively affects regenerative properties of human amniotic epithelial cells in the context of lung repair

2020 ◽  
Vol 134 (20) ◽  
pp. 2665-2679
Author(s):  
Dandan Zhu ◽  
Gina D. Kusuma ◽  
Renate Schwab ◽  
Siow Teng Chan ◽  
Jean Tan ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Progenitor Cells ◽  
Alveolar Epithelial Cells ◽  
Expression Levels ◽  
Alveolar Epithelial ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells ◽  
Average Size ◽  
The Impact

Abstract There is a growing appreciation of the role of lung stem/progenitor cells in the development and perpetuation of chronic lung disease including idiopathic pulmonary fibrosis. Human amniotic epithelial cells (hAECs) were previously shown to improve lung architecture in bleomycin-induced lung injury, with the further suggestion that hAECs obtained from term pregnancies possessed superior anti-fibrotic properties compared with their preterm counterparts. In the present study, we aimed to elucidate the differential effects of hAECs from term and preterm pregnancies on lung stem/progenitor cells involved in the repair. Here we showed that term hAECs were better able to activate bronchioalveolar stem cells (BASCs) and type 2 alveolar epithelial cells (AT2s) compared with preterm hAECs following bleomycin challenge. Further, we observed that term hAECs restored TGIF1 and TGFβ2 expression levels, while increasing c-MYC expression despite an absence of significant changes to Wnt/β-catenin signaling. In vitro, term hAECs increased the average size and numbers of BASC and AT2 colonies. The gene expression levels of Wnt ligands were higher in term hAECs, and the expression levels of BMP4, CCND1 and CDC42 were only increased in the BASC and AT2 organoids co-cultured with hAECs from term pregnancies but not preterm pregnancies. In conclusion, term hAECs were more efficient at activating the BASC niche compared with preterm hAECs. The impact of gestational age and/or complications leading to preterm delivery should be considered when applying hAECs and other gestational tissue-derived stem and stem-like cells therapeutically.

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