scholarly journals Chronological change of gene expression during the transdifferentiation from human amniotic epithelial cells to pancreatic endocrine cells in vitro

Cell Research ◽  
2008 ◽  
Vol 18 (S1) ◽  
pp. S158-S158
Author(s):  
Jian Wang ◽  
Lin Peng ◽  
Xiao Liu ◽  
Guangxiu Lu
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Endocrine Cells ◽  
Chronological Change ◽  
Amniotic Epithelial Cells ◽  
Pancreatic Endocrine Cells ◽  
Human Amniotic Epithelial Cells

scholarly journals Hyaluronic acid ameliorates the proliferative ability of human amniotic epithelial cells through activation of TGF-β/BMP signaling

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10104
Author(s):  
Ya-Bing Tian ◽  
Nuo-Xin Wang ◽  
Yan Xu ◽  
Chang-Yin Yu ◽  
Ru-Ming Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hyaluronic Acid ◽  
Epithelial Cells ◽  
Bmp Signaling ◽  
Inflammatory Factors ◽  
Proliferative Effect ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells ◽  
Proliferative Ability

Human amniotic epithelial cells (hAECs) are a useful and noncontroversial source of stem cells for cell therapy and regenerative medicine, but their limited proliferative ability hinders the acquisition of adequate quantities of cells for clinical use due to not expressing telomerase in hAECs. Our previous study showed that hyaluronic acid (HA), an important component of the extracellular matrix, promoted the proliferation of human amniotic mesenchymal stem cells. Herein, we hypothesize that HA might improve the proliferative capability of hAECs. In the present study, the role of HA on the proliferation of human amniotic epithelial cells (hAECs) in vitro was investigated for the first time. HA at molecular weight of 300 kDa showed an obvious pro-proliferation effect on hAECs. Furthermore, HA not only kept phenotypic characteristics and differentiation capabilities of hAECs, but significantly promoted the secretion of the anti-inflammatory factors such as IL-10 and TGF-β1, and the expression of stem cell pluripotent factors such as Oct4 and Nanog. Analysis of PCR microarray data and RT-qPCR validation showed that TGF-β/BMP signaling was activated in the presence of HA. Further study showed that SB431542, an inhibitor of the TGF-β/BMP signaling, significantly suppressed the mRNA expression of TGFBR3, BMP4, BMP7, BMPR1B, SMAD3, SMAD4, and the pro-proliferative effect of HA on hAECs. These data suggest that HA is a safe and effective enhancer for in vitro expansion of hAECs, whose regulatory mechanism involves the TGF-β/BMP signaling.

scholarly journals In vitro differentiation of human amniotic epithelial cells into insulin-producing 3D spheroids

2015 ◽  
Vol 28 (3) ◽  
pp. 390-402 ◽  
Author(s):  
Bernard Okere ◽  
Francesco Alviano ◽  
Roberta Costa ◽  
Daniela Quaglino ◽  
Francesca Ricci ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
In Vitro Differentiation ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells ◽  
3D Spheroids

scholarly journals Hepatocyte-Like Cells Derived from Human Amniotic Epithelial Cells Can Be Encapsulated Without Loss of Viability or Function In Vitro

2014 ◽  
Vol 23 (8) ◽  
pp. 866-876 ◽  
Author(s):  
Vijesh Vaghjiani ◽  
Vijayaganapathy Vaithilingam ◽  
Indah Saraswati ◽  
Adnan Sali ◽  
Padma Murthi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells
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