scholarly journals Role of Casein Kinase 2 (CK2) for the Progression of Cell Cycle and Growth Control

2008 ◽  
Vol 9 (1) ◽  
pp. 8-12
Author(s):  
Miwako Kato Homma
Keyword(s):  
Cell Cycle ◽  
Growth Control ◽  
Casein Kinase ◽  
Casein Kinase 2

scholarly journals Expression of the Casein Kinase 2 Subunits in Chinese Hamster Ovary and 3T3 L1 Cells Provides Information on the Role of the Enzyme in Cell Proliferation and the Cell Cycle

1999 ◽  
Vol 274 (46) ◽  
pp. 32988-32996 ◽  
Author(s):  
Dongxia Li ◽  
Grazyna Dobrowolska ◽  
Lauri D. Aicher ◽  
Mingzi Chen ◽  
Jocelyn H. Wright ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Casein Kinase ◽  
Casein Kinase 2

Novel Anthraquinone Derivatives as Dual Inhibitors of Topoisomerase 2 and Casein Kinase 2: In Silico Studies, Synthesis and Biological Evaluation on Leukemic Cell Lines

2019 ◽  
Vol 18 (11) ◽  
pp. 1551-1562 ◽  
Author(s):  
Abbas Kabir ◽  
Kalpana Tilekar ◽  
Neha Upadhyay ◽  
C.S. Ramaa
Keyword(s):  
Cell Cycle ◽  
Cell Line ◽  
Cell Lines ◽  
Biological Evaluation ◽  
Casein Kinase ◽  
Casein Kinase 2 ◽  
Cell Cycle Analysis ◽  
Protein Targets ◽  
Topoisomerase 2 ◽  
Dual Inhibitors

Background: Cancer being a complex disease, single targeting agents remain unsuccessful. This calls for “multiple targeting”, wherein a single drug is so designed that it will modulate the activity of multiple protein targets. Topoisomerase 2 (Top2) helps in removing DNA tangles and super-coiling during cellular replication, Casein Kinase 2 (CK2) is involved in the phosphorylation of a multitude of protein targets. Thus, in the present work, we have tried to develop dual inhibitors of Top2 and CK2. Objective: With this view, in the present work, 2 human proteins, Top2 and CK2 have been targeted to achieve the anti-proliferative effects. Methods: Novel 1-acetylamidoanthraquinone (3a-3y) derivatives were designed, synthesized and their structures were elucidated by analytical and spectral characterization techniques (FTIR, 1H NMR, 13C NMR and Mass Spectroscopy). The synthesized compounds were then subjected to evaluation of cytotoxic potential by the Sulforhodamine B (SRB) protein assay, using HL60 and K562 cell lines. Ten compounds were analyzed for Top2, CK2 enzyme inhibitory potential. Further, top three compounds were subjected to cell cycle analysis. Results: The compounds 3a to 3c, 3e, 3f, 3i to 3p, 3t and 3x showed excellent cytotoxic activity to HL-60 cell line indicating their high anti-proliferative potential in AML. The compounds 3a to 3c, 3e, 3f, 3i to 3p and 3y have shown good to moderate activity on K-562 cell line. Compounds 3e, 3f, 3i, 3x and 3y were found more cytotoxic than standard doxorubicin. In cell cycle analysis, the cells (79-85%) were found to arrest in the G0/G1 phase. Conclusion: We have successfully designed, synthesized, purified and structurally characterized 1- acetylamidoanthraquinone derivatives. Even though our compounds need design optimization to further increase enzyme inhibition, their overall anti-proliferative effects were found to be encouraging.

Role of phosphorylated aminoacyl residues in generating atypical consensus sequences which are recognized by casein kinase-2 but not by casein kinase-1

Biochemistry ◽  
1992 ◽  
Vol 31 (25) ◽  
pp. 5893-5897 ◽  
Author(s):  
John W. Perich ◽  
Flavio Meggio ◽  
Eric C. Reynolds ◽  
Oriano Marin ◽  
Lorenzo A. Pinna
Keyword(s):  
Casein Kinase ◽  
Casein Kinase 2 ◽  
Casein Kinase 1 ◽  
Consensus Sequences

scholarly journals Phosphorylation by Casein Kinase 2 Regulates Nap1 Localization and Function

2007 ◽  
Vol 28 (4) ◽  
pp. 1313-1325 ◽  
Author(s):  
Meredith E. K. Calvert ◽  
Kristin M. Keck ◽  
Celeste Ptak ◽  
Jeffrey Shabanowitz ◽  
Donald F. Hunt ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Casein Kinase ◽  
Casein Kinase 2 ◽  
S Phase ◽  
Bud Formation ◽  
Evolutionarily Conserved ◽  
Assembly Factor ◽  
And Function ◽  
Cycle Regulation

ABSTRACT In Saccharomyces cerevisiae, the evolutionarily conserved nucleocytoplasmic shuttling protein Nap1 is a cofactor for the import of histones H2A and H2B, a chromatin assembly factor and a mitotic factor involved in regulation of bud formation. To understand the mechanism by which Nap1 function is regulated, Nap1-interacting factors were isolated and identified by mass spectrometry. We identified several kinases among these proteins, including casein kinase 2 (CK2), and a new bud neck-associated protein, Nba1. Consistent with our identification of the Nap1-interacting kinases, we showed that Nap1 is phosphorylated in vivo at 11 sites and that Nap1 is phosphorylated by CK2 at three substrate serines. Phosphorylation of these serines was not necessary for normal bud formation, but mutation of these serines to either alanine or aspartic acid resulted in cell cycle changes, including a prolonged S phase, suggesting that reversible phosphorylation by CK2 is important for cell cycle regulation. Nap1 can shuttle between the nucleus and cytoplasm, and we also showed that CK2 phosphorylation promotes the import of Nap1 into the nucleus. In conclusion, our data show that Nap1 phosphorylation by CK2 appears to regulate Nap1 localization and is required for normal progression through S phase.

The role of casein kinase 2 in ER stress associated pancreatic β cell failure

2016 ◽  
Vol 120 ◽  
pp. S42
Author(s):  
Yuki Matsuura ◽  
Tomoko Takai ◽  
Tomokazu Matsuda ◽  
Emi Terashi ◽  
Ayumi Kanno ◽  
...  
Keyword(s):  
Er Stress ◽  
Casein Kinase ◽  
Casein Kinase 2 ◽  
Pancreatic Β Cell ◽  
Β Cell

scholarly journals Biochemical and Genetic Analyses of the Role of Yeast Casein Kinase 2 in Salt Tolerance

1999 ◽  
Vol 181 (20) ◽  
pp. 6456-6462 ◽  
Author(s):  
Eulàlia de Nadal ◽  
Fernando Calero ◽  
José Ramos ◽  
Joaquín Ariño
Keyword(s):  
Casein Kinase ◽  
Salt Sensitivity ◽  
Casein Kinase 2 ◽  
Regulatory Subunit ◽  
Wild Type ◽  
Sodium Efflux ◽  
Regulatory Pathways ◽  
Essentially Normal ◽  
Intracellular Content

ABSTRACT Saccharomyces cerevisiae cells lacking the regulatory subunit of casein kinase 2 (CK-2), encoded by the geneCKB1, display a phenotype of hypersensitivity to Na+ and Li+ cations. The sensitivity of a strain lacking ckb1 is higher than that of a calcineurin mutant and similar to that of a strain lacking HAL3, the regulatory subunit of the Ppz1 protein phosphatase. Genetic analysis indicated that Ckb1 participates in regulatory pathways different from that of Ppz1 or calcineurin. Deletion of CKB1 increased the salt sensitivity of a strain lacking Ena1 ATPase, the major determinant for sodium efflux, suggesting that the function of the kinase is not mediated by Ena1. Consistently, ckb1 mutants did not show an altered cation efflux. The function of Ckb1 was independent of theTRK system, which is responsible for discrimination of potassium and sodium entry, and in the absence of the kinase regulatory subunit, the influx of sodium was essentially normal. Therefore, the salt sensitivity of a ckb1 mutant cannot be attributed to defects in the fluxes of sodium. In fact, in these cells, both the intracellular content and the cytoplasm/vacuole ratio for sodium were similar to those features of wild-type cells. The possible causes for the salt sensitivity phenotype of casein kinase mutants are discussed in the light of these findings.

179 Pro-apototic role of casein kinase 2 is mediated by a JNK signaling cascade

Hepatology ◽  
2003 ◽  
Vol 38 ◽  
pp. 241-241
Author(s):  
P HILGARD ◽  
G GERKEN ◽  
M CZAJA ◽  
R STOCKERT
Keyword(s):  
Casein Kinase ◽  
Casein Kinase 2 ◽  
Signaling Cascade ◽  
Jnk Signaling
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