Role of phosphorylated aminoacyl residues in generating atypical consensus sequences which are recognized by casein kinase-2 but not by casein kinase-1

Biochemistry ◽  
1992 ◽  
Vol 31 (25) ◽  
pp. 5893-5897 ◽  
Author(s):  
John W. Perich ◽  
Flavio Meggio ◽  
Eric C. Reynolds ◽  
Oriano Marin ◽  
Lorenzo A. Pinna
Keyword(s):  
Casein Kinase ◽  
Casein Kinase 2 ◽  
Casein Kinase 1 ◽  
Consensus Sequences

scholarly journals Dangerous Duplicity: The Dual Functions of Casein Kinase 1in Parasite Biology and Host Subversion

2021 ◽  
Vol 11 ◽  
Author(s):  
Najma Rachidi ◽  
Uwe Knippschild ◽  
Gerald F. Späth
Keyword(s):  
Current Knowledge ◽  
Family Members ◽  
Casein Kinase ◽  
Biological Processes ◽  
Casein Kinase 1 ◽  
Parasite Survival ◽  
Host Pathogen ◽  
Parasite Biology ◽  
Antimicrobial Interventions

Casein Kinase 1 (CK1) family members are serine/threonine protein kinases that are involved in many biological processes and highly conserved in eukaryotes from protozoan to humans. Even though pathogens exploit host CK1 signaling pathways to survive, the role of CK1 in infectious diseases and host/pathogen interaction is less well characterized compared to other diseases, such as cancer or neurodegenerative diseases. Here we present the current knowledge on CK1 in protozoan parasites highlighting their essential role for parasite survival and their importance for host-pathogen interactions. We also discuss how the dual requirement of CK1 family members for parasite biological processes and host subversion could be exploited to identify novel antimicrobial interventions.

The role of casein kinase 2 in ER stress associated pancreatic β cell failure

2016 ◽  
Vol 120 ◽  
pp. S42
Author(s):  
Yuki Matsuura ◽  
Tomoko Takai ◽  
Tomokazu Matsuda ◽  
Emi Terashi ◽  
Ayumi Kanno ◽  
...  
Keyword(s):  
Er Stress ◽  
Casein Kinase ◽  
Casein Kinase 2 ◽  
Pancreatic Β Cell ◽  
Β Cell

scholarly journals Expression of the Casein Kinase 2 Subunits in Chinese Hamster Ovary and 3T3 L1 Cells Provides Information on the Role of the Enzyme in Cell Proliferation and the Cell Cycle

1999 ◽  
Vol 274 (46) ◽  
pp. 32988-32996 ◽  
Author(s):  
Dongxia Li ◽  
Grazyna Dobrowolska ◽  
Lauri D. Aicher ◽  
Mingzi Chen ◽  
Jocelyn H. Wright ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Casein Kinase ◽  
Casein Kinase 2

scholarly journals Casein Kinase-1-Alpha Inhibitor (D4476) Sensitizes Microsatellite Instable Colorectal Cancer Cells to 5-Fluorouracil via Authophagy Flux Inhibition

2021 ◽  
Vol 69 (1) ◽  
Author(s):  
Morvarid Siri ◽  
Hamid Behrouj ◽  
Sanaz Dastghaib ◽  
Mozhdeh Zamani ◽  
Wirginia Likus ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Chemotherapy Resistance ◽  
Casein Kinase ◽  
Autophagy Flux ◽  
Casein Kinase 1 ◽  
Colorectal Cancer Cells ◽  
High Level ◽  
Casein Kinase 1 Alpha ◽  
Hct116 Cells

AbstractAdjuvant chemotherapy with 5-fluorouracil (5-FU) does not improve survival of patients suffering from a form of colorectal cancer (CRC) characterized by high level of microsatellite instability (MSI-H). Given the importance of autophagy and multi-drug-resistant (MDR) proteins in chemotherapy resistance, as well as the role of casein kinase 1-alpha (CK1α) in the regulation of autophagy, we tested the combined effect of 5-FU and CK1α inhibitor (D4476) on HCT116 cells as a model of MSI-H colorectal cancer. To achieve this goal, the gene expression of Beclin1 and MDR genes, ABCG2 and ABCC3 were analyzed using quantitative real-time polymerase chain reaction. We used immunoblotting to measure autophagy flux (LC3, p62) and flow cytometry to detect apoptosis. Our findings showed that combination treatment with 5-FU and D4476 inhibited autophagy flux. Moreover, 5-FU and D4476 combination therapy induced G2, S and G1 phase arrests and it depleted mRNA of both cell proliferation-related genes and MDR-related genes (ABCG2, cyclin D1 and c-myc). Hence, our data indicates that targeting of CK1α may increase the sensitivity of HCT116 cells to 5-FU. To our knowledge, this is the first description of sensitization of CRC cells to 5-FU chemotherapy by CK1α inhibitor. Graphic abstract

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