scholarly journals Immobilization of Aspergillus Niger cellulase onto Lifetech TM carriers and its application in the hydrolysis of sunflower seed meal lignocellulosic fraction

2019 ◽  
Vol 46 (2) ◽  
pp. 161-169
Author(s):  
Marija Ćorović ◽  
Milica Simović ◽  
Ana Milivojević ◽  
Katarina Banjanac ◽  
Katarina Katić ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Sunflower Seed ◽  
Seed Meal ◽  
Hydrolysis Of

scholarly journals Hydrolysis of sunflower seed meal lignocellulosic fraction by free and immobilized cellulases

2020 ◽  
Vol 11 (15) ◽  
Author(s):  
Marija Ćorović
Keyword(s):  
Sunflower Seed ◽  
Catalytic Efficiency ◽  
Cost Effective ◽  
Reaction Rates ◽  
Seed Meal ◽  
Initial Reaction ◽  
Amino Groups ◽  
Spacer Arm ◽  
Optimized Conditions ◽  
Hydrolysis Of

Lignocellulosic biomass is widely abundant in nature and recognized aspotential renewable energy source. Its efficient transformation into bio-basedfuels is enabled only after adequate pretreatment, followed by enzymaticsacharification and microbial fermentation. Hereby we present application of twocellulase preparations – from Aspergillus niger and Trichoderma reesei (Celluclast®)in treating sunflower seed meal lignocellulosic fraction (SSMLF). Temperature andpH optimums of two enzymes were determined – 52 °C and pH4.8 for A. nigercellulase and 55 °C and pH4.5 for Celluclast®. At optimized conditions, milledSSMLF was hydrolyzed by both biocatalysts. With A. niger cellulase higher initialreaction rates were accomplished and yield of 70 mM glucose equivalent wasobtained with 6 % (w/v) of enzyme after 6 hours. On the other hand, applicationof Celluclast® led to lower initial reaction rates and yielded 25 mM of glucoseequivalent with 10 % (v/v) of enzyme. To ensure cost-effective application ofA. niger cellulase, the possibility of its immobilization on different supports wasinvestigated. By using porous methacrylate-based carrier with C6 spacer arm andprimary amino groups – LifetechTM ECR8409, preparation with highest activity wasproduced. This preparation was successfully applied in saccharification of SSMLFand showed unchanged catalytic efficiency comparing to free enzyme.

scholarly journals Improvement of Enzymatic Saccharification of Cellulose-Containing Raw Materials Using Aspergillus niger

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1360
Author(s):  
Ekaterina Budenkova ◽  
Stanislav Sukhikh ◽  
Svetlana Ivanova ◽  
Olga Babich ◽  
Vyacheslav Dolganyuk ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Aspergillus Niger ◽  
Filter Paper ◽  
Raw Materials ◽  
Enzymatic Saccharification ◽  
Wood Chip ◽  
Cellulase Activity ◽  
Wood Chips ◽  
Acid Pretreatment ◽  
Hydrolysis Of

Enzymatic hydrolysis of cellulose-containing raw materials, using Aspergillus niger, were studied. Filter paper, secondary cellulose-containing or starch-containing raw materials, miscanthus cellulose after alkaline or acid pretreatment, and wood chip cellulose, were used as substrates. The study focused on a wild A. niger strain, treated, or not (control), by ultraviolet (UV) irradiations for 45, 60, or 120 min (UV45, UV60, or UV120), or by UV irradiation for 120 min followed by a chemical treatment with NaN3 + ItBr for 30 min or 80 min (UV120 + CH30 or UV120 + CH80). A mixture of all the A. niger strains (MIX) was also tested. A citrate buffer, at 50 mM, wasthe most suitable for enzymatic hydrolysis. As the UV exposure time increased to 2 h, the cellulase activity of the surviving culturewas increased (r = 0.706; p < 0.05). The enzymatic activities of the obtained strains, towards miscanthus cellulose, wood chips, and filter paper, were inferior to those obtained with commercial enzymes (8.6 versus 9.1 IU), in some cases. Under stationary hydrolysis at 37 °C, pH = 4.7, the enzymatic activity of A. niger UV120 + CH30 was 24.9 IU. The enzymatic hydrolysis of secondary raw materials, using treated A. niger strains, was themost effective at 37 °C. Similarly, the most effective treatment of miscanthus cellulose and wood chips occurred at 50 °C. The maximum conversion of cellulose to glucose was observed using miscanthus cellulose (with alkaline pretreatment), and the minimum conversion was observed when using wood chips. The greatest value of cellulase activity was evidenced in the starch-containing raw materials, indicating that A. niger can ferment not only through cellulase activity, but also via an amylolytic one.

Prevalence of Aspergillus species on selected Colombian animal feedstuffs and ability of Aspergillus section Flavi to produce aflatoxins

2009 ◽  
Vol 2 (1) ◽  
pp. 31-34 ◽  
Author(s):  
G. Diaz ◽  
M. Lozano ◽  
A. Acuña
Keyword(s):  
Liquid Chromatography ◽  
Cottonseed Meal ◽  
Sunflower Seed ◽  
Seed Meal ◽  
Aspergillus Section Flavi ◽  
Wheat Middlings ◽  
Highperformance Liquid Chromatography ◽  
Animal Feedstuffs ◽  
Aflatoxin B ◽  
Aspergillus Section

A total of 57 samples of feedstuffs commonly used for animal nutrition in Colombia (maize, soybean, sorghum, cottonseed meal, sunflower seed meal, wheat middlings and rice) were analysed for Aspergillus contamination. Aspergillus fungi were identified at species level and their ability to produce aflatoxins was determined by highperformance liquid chromatography. A total of 31 of the feedstuffs analysed (54.4%) were found to contain Aspergillus spp. The most contaminated substrate was maize (100%) followed by cottonseed meal (80%), sorghum (60%) and wheat middlings (60%). Soybean showed lower levels of contamination (10%). No Aspergillus spp. could be isolated from rice or sunflower seed meal. Total Aspergillus strains isolated were 50, with 28 belonging to section Flavi (56%), 17 to section Nigri (34%), 4 to section Circumdati (8%) and 1 to section Fumigati (2%). Among section Flavi, 17 isolates were identified as A. flavus, seven as A. parasiticus, two as A. oryzae and two as A. tamarii. Production of aflatoxins by Aspergillus section Flavi was screened by liquid chromatography. About three quarters of the A. flavus strains (76.5%) produced aflatoxin B1 (0.2 to 240.4 µg/g) and aflatoxin B2 (0.2 to 1.6 µg/g), while all A. parasiticus strains produced the four naturally occurring aflatoxins (aflatoxin B1 from 0.6 to 83.5 µg/g, aflatoxin B2 from 0.3 to 4.8 µg/g, aflatoxin G1 from 0.4 to 19.3 µg/g and aflatoxin G2 from 0.1 to 1.0 µg/g). This is the first study demonstrating the presence of highly toxigenic Aspergillus fungi in Colombian animal feedstuffs.

scholarly journals EFFECT OF VARIOUS DIETARY LEVELS OF SUNFLOWER SEED MEAL ON THE PERFORMANCE OF GROWING RABBITS IN SUDANO-SAHELIAN ZONE

2021 ◽  
Vol 22 (2) ◽  
pp. 150-154
Author(s):  
A. C. Okonkwo ◽  
J. U. Igwebuike ◽  
H. U. Tarfa ◽  
B. I. Okon ◽  
B. I. Umoh
Keyword(s):  
Deleterious Effect ◽  
Sunflower Seed ◽  
Crude Protein ◽  
Seed Meal ◽  
Small Scale ◽  
Considerable Decrease ◽  
Feed Cost ◽  
Significant Difference ◽  
Ad Libitum ◽  
Dietary Treatments

The experiment which lasted for seventy (70) days was conducted to determine the effects of various dietary levels of sunflower seed meal (SSM) on the performance of growing rabbits. Thirty- two (32) indigenous breeds of rabbits obtained from small-scale producers were used in study. They were individually weighed and randomly assigned to the four (4) dietary treatments, in which 0,10, 20 and 30% of the groundnut cake was replaced by SSM, respectively. The diets contained 2391.76, 2387.44, 2383.12 and 2378.80 Kcal ME/kg for diets 1,2,3 and 4, respectively. The crude protein (CP) was approximately 14.0% for the four (4) experimental diets. The diets, in mash form were fed to the rabbits ad libitum. There was no significant difference (P > 0.05) amongst the treatments in all the parameters examined. However, he incorporation of SSM resulted in a considerable decrease in feed cost with increasing levels of SSM. Thc. results of this Study therefore suggest that 30% or more of the GNC could be replaced by SSM in the diets of growing rabbits without deleterious effect on performance.

scholarly journals Short communication: Pelleting increases the metabolizable energy of de-hulled sunflower seed meal for broilers

2021 ◽  
Vol 51 (3) ◽  
pp. 290-295
Author(s):  
N. Karkelanov ◽  
S. Chobanova ◽  
I.M. Whiting ◽  
K. Dimitrova ◽  
S.P. Rose ◽  
...  
Keyword(s):  
Sunflower Seed ◽  
Dry Matter ◽  
Short Communication ◽  
Nutrient Retention ◽  
Metabolizable Energy ◽  
Seed Meal ◽  
Sunflower Seeds ◽  
Apparent Metabolizable Energy ◽  
Pass Through ◽  
Broiler Feed

The study examined the effects of two methods of processing de-hulled sunflower seed meal (SFM) from the same batch of sunflower seeds. Sunflower seed meal was fed to broilers as meal (MSFM) or after it had been pelleted (PSFM) at 75 °C and 360 kPa pressure to pass through a 3 mm mesh. Three diets were prepared, namely a balancer feed (BF) and two diets containing 200 g/kg MSFM or 200 g/kg PSFM. They were fed to 30 pens (two birds each) with male Ross 308 broilers, from 8 to 21 days old, following randomization. Data were analysed by ANOVA. Two pre-planned orthogonal contrast tests were performed to compare overall differences between the diets containing SFM and BF and between diets containing the MSFM and PSFM. The BF had a very different nutrient composition from the complete feeds containing SFM so, as expected, there were differences in growth performance and nutrient retention. The diet containing PSFM had greater apparent metabolizable energy corrected for N retention (AMEn) and dry matter retention (DMR) than that containing MSFM. The use of the substitution method showed the PSFM had AMEn that was 18% greater than the MSFM (8.79 vs 7.47 MJ/kg DM). Under the conditions of the current study, incorporating PSFM in a mash broiler feed increased dietary AMEn compared with the same feed containing MSFM. Further studies are needed to identify whether the benefits of pre-pelleting SFM remain after this product has been incorporated in complete pelleted broiler feeds.

scholarly journals Production of Xylo-oligosaccharides (XOS) by controlled hydrolysis of Xylan using immobilized Xylanase from Aspergillus niger with improved properties

2018 ◽  
Vol 5 (4) ◽  
Author(s):  
Caio C. Aragon ◽  
Ana I. Ruiz-Matute ◽  
Nieves Corzo ◽  
Rubens Monti ◽  
Jose M. Guisán ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Hydrolysis Of ◽  
Controlled Hydrolysis

scholarly journals Hydrolysis of Phytic Acid in Brown-Rice Bread by Aspergillus niger Phytase

2005 ◽  
Vol 58 (5) ◽  
pp. 267-272 ◽  
Author(s):  
Akiko Matsuo ◽  
Kenji Sato ◽  
Yasushi Nakamura ◽  
Kozo Ohtsuki
Keyword(s):  
Aspergillus Niger ◽  
Phytic Acid ◽  
Brown Rice ◽  
Hydrolysis Of ◽  
Aspergillus Niger Phytase
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