scholarly journals LIMITING FACTORS IN SINGLE PARTICLE CRYO ELECTRON TOMOGRAPHY

2012 ◽  
Vol 1 (2) ◽  
pp. e201207002 ◽  
Author(s):  
Mikhail Kudryashev ◽  
Daniel Castaño-Díez ◽  
Henning Stahlberg
Keyword(s):  
Single Particle ◽  
Electron Tomography ◽  
Limiting Factors ◽  
Cryo Electron Tomography

scholarly journals Phase Contrast Cryo-Electron Tomography and Single Particle Analysis with a New Phase Plate

2014 ◽  
Vol 20 (S3) ◽  
pp. 232-233
Author(s):  
Maryam Khoshouei ◽  
Radostin Danev ◽  
Günther Gerisch ◽  
Maria Ecke ◽  
Juergen Plitzko ◽  
...  
Keyword(s):  
Single Particle ◽  
Phase Contrast ◽  
Electron Tomography ◽  
Particle Analysis ◽  
Phase Plate ◽  
Single Particle Analysis ◽  
Cryo Electron Tomography ◽  
New Phase

scholarly journals Alignment algorithms and per-particle CTF correction for single particle cryo-electron tomography

2016 ◽  
Vol 194 (3) ◽  
pp. 383-394 ◽  
Author(s):  
Jesús G. Galaz-Montoya ◽  
Corey W. Hecksel ◽  
Philip R. Baldwin ◽  
Eryu Wang ◽  
Scott C. Weaver ◽  
...  
Keyword(s):  
Single Particle ◽  
Electron Tomography ◽  
Alignment Algorithms ◽  
Cryo Electron Tomography

scholarly journals Routine Single Particle CryoEM Sample and Grid Characterization by Tomography

2017 ◽  
Author(s):  
Alex J. Noble ◽  
Venkata P. Dandey ◽  
Hui Wei ◽  
Julia Brasch ◽  
Jillian Chase ◽  
...  
Keyword(s):  
Single Particle ◽  
Particle Distribution ◽  
De Novo ◽  
Protein Denaturation ◽  
Electron Tomography ◽  
Particle Collection ◽  
Cryo Electron Microscopy ◽  
Cryo Electron Tomography ◽  
Air Water Interface ◽  
Particle Alignment

AbstractSingle particle cryo-electron microscopy (cryoEM) is often performed under the assumption that particles are freely floating away from the air-water interfaces and in thin, vitreous ice. In this study, we performed fiducial-less tomography on over 50 different cryoEM grid/sample preparations to determine the particle distribution within the ice and the overall geometry of the ice in grid holes. Surprisingly, by studying particles in holes in 3D from over 1,000 tomograms, we have determined that the vast majority of particles (approximately 90%) are adsorbed to an air-water interface. The implications of this observation are wide-ranging, with potential ramifications regarding protein denaturation, conformational change, and preferred orientation. We also show that fiducial-less cryo-electron tomography on single particle grids may be used to determine ice thickness, optimal single particle collection areas and strategies, particle heterogeneity, and de novo models for template picking and single particle alignment.

Quantifying resolution limiting factors in subtomogram averaged cryo-electron tomography using simulations

2014 ◽  
Vol 187 (2) ◽  
pp. 103-111 ◽  
Author(s):  
Lenard M. Voortman ◽  
Miloš Vulović ◽  
Massimiliano Maletta ◽  
Andreas Voigt ◽  
Erik M. Franken ◽  
...  
Keyword(s):  
Electron Tomography ◽  
Limiting Factors ◽  
Cryo Electron Tomography
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