Quantifying resolution limiting factors in subtomogram averaged cryo-electron tomography using simulations

2014 ◽  
Vol 187 (2) ◽  
pp. 103-111 ◽  
Author(s):  
Lenard M. Voortman ◽  
Miloš Vulović ◽  
Massimiliano Maletta ◽  
Andreas Voigt ◽  
Erik M. Franken ◽  
...  
Keyword(s):  
Electron Tomography ◽  
Limiting Factors ◽  
Cryo Electron Tomography

scholarly journals LIMITING FACTORS IN SINGLE PARTICLE CRYO ELECTRON TOMOGRAPHY

2012 ◽  
Vol 1 (2) ◽  
pp. e201207002 ◽  
Author(s):  
Mikhail Kudryashev ◽  
Daniel Castaño-Díez ◽  
Henning Stahlberg
Keyword(s):  
Single Particle ◽  
Electron Tomography ◽  
Limiting Factors ◽  
Cryo Electron Tomography

scholarly journals Determining the Patchwork Lattice of Ebola and Marburg Virus Matrix Layers Using Cryo-Electron Tomography

2021 ◽  
Vol 27 (S1) ◽  
pp. 1884-1884
Author(s):  
William Wan ◽  
Mairi Clarke ◽  
Michael Norris ◽  
Larissa Kolesnikova ◽  
Alexander Koehler ◽  
...  
Keyword(s):  
Electron Tomography ◽  
Marburg Virus ◽  
Cryo Electron Tomography

scholarly journals Tubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility

Science ◽  
2021 ◽  
Vol 371 (6525) ◽  
pp. eabd4914
Author(s):  
Sudarshan Gadadhar ◽  
Gonzalo Alvarez Viar ◽  
Jan Niklas Hansen ◽  
An Gong ◽  
Aleksandr Kostarev ◽  
...  
Keyword(s):  
Posttranslational Modifications ◽  
Male Fertility ◽  
Electron Tomography ◽  
Structural Basis ◽  
Cellular Functions ◽  
Flagellar Beat ◽  
Cryo Electron Tomography ◽  
Axonemal Dynein ◽  
Dynein Arms ◽  
Cilia And Flagella

Posttranslational modifications of the microtubule cytoskeleton have emerged as key regulators of cellular functions, and their perturbations have been linked to a growing number of human pathologies. Tubulin glycylation modifies microtubules specifically in cilia and flagella, but its functional and mechanistic roles remain unclear. In this study, we generated a mouse model entirely lacking tubulin glycylation. Male mice were subfertile owing to aberrant beat patterns of their sperm flagella, which impeded the straight swimming of sperm cells. Using cryo–electron tomography, we showed that lack of glycylation caused abnormal conformations of the dynein arms within sperm axonemes, providing the structural basis for the observed dysfunction. Our findings reveal the importance of microtubule glycylation for controlled flagellar beating, directional sperm swimming, and male fertility.

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