scholarly journals Sanidade e qualidade fisiológica de sementes de Chorisia glaziovii O. Kuntze tratadas com extratos vegetais

2019 ◽  
Vol 29 (2) ◽  
pp. 649
Author(s):  
Andrezza Klyvia Oliveira Araújo ◽  
Maria Lúcia Maurício Silva ◽  
Rommel Do Santos Siqueira Gomes ◽  
Angeline Maria da Silva Santos ◽  
Luciana Cordeiro do Nascimento

O objetivo da pesquisa foi avaliar a eficiência dos extratos de alho e erva-cidreira sobre patógenos e na qualidade fisiológica de sementes de Chorisia glaziovii. Os tratamentos constituíram-se de extratos de alho e erva-cidreira à 0, 25, 50, 75 e 100%, e fungicida Captan®. Para sanidade das sementes adotou-se o método blotter test e a qualidade fisiológica foi determinada pela germinação e vigor. O delineamento foi inteiramente casualizados em esquema fatorial (2x5) +1 (extratos vegetais x concentrações + fungicida), com quatro repetições. Observou-se a ocorrência de Aspergillus flavus, Aspergillus niger, Aspergillus sp., Botrytis sp., Cladosporium sp., Curvularia lunata, Nigrospora sp., Pestalotia sp., Periconia sp. e Rhizopus stolonifer, nas sementes. O extrato de erva-cidreira à 100% foi eficiente na inibição dos patógenos. O aumento na concentração do extrato de alho foi prejudicial à primeira contagem de germinação, e o extrato de erva-cidreira proporcionou aumento de sementes germinadas.

Author(s):  
A Khatun ◽  
S Shamsi ◽  
MA Bashar

Present paper deals with the occurrence and diversity of fungi on storage cotton (Gossypium hirsutum L.) seeds. 24 species of the fungi were found associated with the seeds of 14 varieties of cotton after 10 months of storage. The isolated fungi were Aspergillus flavus Link, A. fumigatus Fresenius, A. niger (Type 1 and Type 2) Van Tiegh, A. ochraceus K. Wilhelm, A. nidulans Eidam, Aspergillus sp.1, Aspergillus sp.2, Aspergillus sp.3, Curvularia lunata (Wakker) Boedijn, Colletotrichum gloeosporioides Penz & Sacc, C. gossypii Southw., Chaetomium globosum Kunze., Fusarium nivale (Fr.) Sorauer, F. moniliforme J. Shelden, F. oxysporum Schlechtendal, F. fujikuroi Nirenberg, Mucor sp. P. Micheli ex L., Penicillium sp.1 and sp.2 Link, Rhizoctonia solani Khun., Rhizopus stolonifer (Ehrenb.) Vuill., Rhizomucor Lucet & Costantin, Syncephalastrum racemosum Cohn and Trichoderma viride Pers. The association of fungi with cotton seeds was recorded within two months of harvest, after 6 and 10 months of storage. The association of fungi varied with duration of storage periods. Aspergillus flavus, A. fumigatus, A. niger, Chaetomium globosum, Penicillium sp.1 and Rhizopus stolonifer were found in all the examined varieties. These were the most predominant fungi in terms of prevalence with the increase of storage periods. The fungal association with the seeds of cotton also affected the germination, seedling mortality, height and vigor. The total association of fungi in cotton seeds was the highest in cotton variety CB10 (204) and the lowest in CB8 (71). On the other hand, the total fungal association of Rhizopus stolonifer was the highest (337) and Colletotrichum gossypii was the lowest (1). J. Biodivers. Conserv. Bioresour. Manag. 2018, 4(2): 43-52


2021 ◽  
Vol 5 (1) ◽  
pp. 86
Author(s):  
Grace Purnamasari Christian ◽  
Wahyu Irawati

<p>The post-harvest fungi is one of the greatest cause of various diseases. Particularly, in fungus that infects seeds, the toxic substance called aflatoxin is found. This has caused us to wonder: Has fungi been pathogenic fungi since the beginning of creation? The purpose of this research is to study: 1) The Place of Fungi in God’s Original Creation and the Origin of Pathogenic Fungi, 2) Characterization of post-harvest fungus on corn seeds, 3) Aspergillus sp.’s Structure as Proof of God’s Wonderful Original Creation and Providence, and 4) The factors that contribute to the growth of fungi in post-harvest corn seeds and our responsibility. We conclude that in the beginning, fungi were a part of God’s originally good and perfect creation. These fungi were created by God with the structure and function to support its operation in fulfilling God’s creative purpose. The complex structure of Aspergillus niger and Aspergillus flavus that infected the corn seeds showed God’s providence after the Fall. Pathogenic fungi were the result of the Fall of man into sin. Their growth is caused by the failure to give proper care to the crops. But this should not discourage us since the Bible tells us that God is continually working to restore His creation. Therefore, we are called to be stewards of His creation, to develop and preserve whatever is entrusted in our hands, including the crops for God’s glory and under His authority until He has fully renewed and restored everything.</p><p><strong>BAHASA INDONESIA ABSTRACT:</strong> Jamur pasca panen merupakan salah satu penyebab terbesar timbulnya berbagai penyakit, khususnya disebabkan oleh jamur yang menginfeksi biji-bijian dan menghasilkan substansi beracun disebut dengan aflatoksin. Keadaan ini menimbulkan pertanyaan apakah jamur patogenik ada sejak awal penciptaan? Penelitian ini bertujuan untuk mempelajari: 1) Posisi jamur patogenik di awal penciptaan dan asal mula jamur patogenik, 2) karakteristik jamur pasca panen pada biji jagung, 3) struktur <em>Aspergillus</em> sp sebagai bukti dari providensi Allah akan ciptaanNya yang sangat luar biasa, 4) faktor-faktor yang berkontribusi terhadap pertumbuhan jamur pasca panen biji jagung dan Tanggung jawab kita. Dari hasil penelitian ini disimpulkan bahwa pada mulanya, jamur merupakan bagian ciptaan Allah yang sungguh amat baik dan sempurna. Struktur yang kompleks dari <em>Aspergillus niger</em> dan <em>Aspergillus flavus </em>yang menginfeksi biji jagung menunjukkan providensi Allah setelah kejatuhan dosa. Pertumbuhan jamur patogenik merupakan gambaran kejatuhan manusia ke dalam dosa yaitu kegagalan manusia untuk memberikan pemeliharaan yang memadahi pada biji-bijian pasca panen. Namun demikian, keadaan ini tidak mematahkan semangat kita karena Alkitab mengatakan bahwa Allah masih terus bekerja untuk memulihkan ciptaanNya. Oleh karena itu, diharapkan kita semua menjadi pelayan atas ciptaanNya untuk mengembangkan dan menjaga apa yang dipercayakan kepada kita di bawah otoritasNya termasuk biji-bijian untuk memuliakan Allah hingga Dia secara penuh memperbaharui dan memulihkan segala sesuatu</p>


2020 ◽  
Vol 3 (1) ◽  
pp. 1-15
Author(s):  
Sifa Sobianti ◽  
Loekas Soesanto ◽  
Suciati Hadi

Penelitian ini bertujuan untuk mengetahui (1) beragam spesies jamur patogen tular-benih pada lima varietas padi, dan (2) persentase daya kecambah lima varietas padi. Penelitian dilaksanakan di Laboratorium Karantina Pertanian Kelas I Semarang, dari bulan Maret sampai dengan Mei 2019. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 5 perlakuan dan 5 ulangan, perlakuan terdiri atas Varietas Inpago Unsoed 1, Inpago Unsoed Parimas, IR64, Ciherang, dan Situ Bagendit. Isolasi dan identifikasi jamur patogen tular-benih dilakukan pada blotter test dan medium PDA, dengan pengamatan di bawah mikroskop stereo dan kompon, kemudian dibandingkan dengan pustaka. Variabel yang diamati yaitu morfologi koloni patogen, morfologi patogen, dan daya kecambah. Hasil penelitian menunjukkan bahwa (1) pada semua varietas benih padi terdapat jamur patogen tular-benih, tetapi tidak semua jamur tersebut dijumpai pada setiap varietas. Jenis jamur patogen tular-benih yang dijumpai, yaitu Alternaria padwickii Ganguly, Aspergillus flavus Link, Aspergillus niger van Tieghem, Curvularia lunata (Wakker) Boedjin, Curvularia pallescens Boedjin, Drechslera oryzae Breda de Haan, Fusarium semitectum Berk. & Rav. [W&R, G,B,J], Rhizoctonia solani J. G. Kuhn, Rhizopus oryzae Went & Prins. Geerl., dan Tilletia barclayana Bref., dan (2) uji daya kecambah menunjukkan bahwa masing-masing varietas benih padi memilki persentase daya kecambah benih yang berbeda. Daya kecambah benih berturut-turut pada varietas IR64, Ciherang, Inpago Unsoed 1, Inpago Unsoed Parimas, dan Situ Bagendit sebesar 85,75, 81,75, 80,25, 76,5, dan 70%.


2018 ◽  
Vol 29 (1) ◽  
pp. 15
Author(s):  
Dina Yousif Mohamed

The aim of this study is to detection the antifungal effect of Zirconium oxide nanoparticles on mold which isolated from domestic's bathroom during April 2017 in Baghdad City. Twenty species were isolated from one hundred samples which were Aspergillus niger, Aspergillus. flavus, , Aspergillus duricaulis, Aspergillus nidulans Aspergillus. parasiticus ,Aspergillus fumigatus, Aspergillus. brasiliensis ,Aspergillus heteromorphus , Curvularia lunata, Penicillium sp., Fusarium oxysporum, , Alternaria alternate, Cladosporium sp. Trichoderma sp., Mucor, Rhizopus sp., Rhodotorula mucilaginosa, , Stachybotrys and yeast. Among the isolated species Aspergillus niger, was the most abundant (14.92%) followed by Aspergillus flavus (10.14%), while less abundant (0.95 & 0.63 %) Cladosporium sp. &, Mucor respectively. The higher demoralization of fungal growth was recorded at 8mg/ml concentration of Zirconium oxide nanoparticles which was (3.8cm) in molds Aspergillus niger, Aspergillus. flavus, , & Aspergillus. brasiliensis


2018 ◽  
Author(s):  
Manjit Kumar Ray ◽  
Piyush Kumar Mishra ◽  
Saurav Das

AbstractSericulture is an important cottage industry of Assam producing natural silk of both Mulberry and Non-Mulberry silk. The sericulture industry is closely associated with the Assamese traditions and rituals. In non-mulberry silk, Muga silkworm which is endemic to North-East India only produce exquisite silks of golden color. Rearing of Muga silkworm is one of the important aspects to producing silk of high quality. The quality of the primary host plant i.e. Som (Persea bombycina Kost.) greatly affects the quality of cocoon and silk produced by these industries. Therefore, planting and growing a disease-free plant has its own importance in sericulture. However, Som is very susceptible to different foliar diseases caused by fungi, which can reduce the yield of leaf from 13.8 - 41.6% annually. Therefore, a comprehensive mycofloral study of the host plant is important to forecast the future diseases and design different disease management procedures. This study has been done for a period of two years from 2014 - 2016 in Goalpara district of Assam and overall mycoflora of pedosphere (rhizosphere and non-rhizosphere), phyllosphere and aerosphere were identified and correlated with the seasonal variation. The rhizosphere, air, and phylloplane was dominated by Rhizopus stolonifer (22.13%; 15.08; 24.01) while Aspergillus niger (12.63%) was the dominant flora of non-rhizosphere. Seasonal variation was found to play an important role in shaping the mycofloral community structure in soil and phyllosphere. In summer, soil was majorly dominated by Aspergillus niger, Aspergillus fumigatus, and Curvularia lunata while Rhizopus stolonifer, Aspergillus clavatus, Penicillium chrysogenum dominated the winter soil. In phyllosphere, the Chatuwa and Jaruwa, the winter generations were mainly dominated by Rhizopus stolonifer. While as the environmental temperature gradually increased with relative humidity in Aheruwa, Bhodia, Kotia and Jethuwa generation there was a shift in diversity with a gradual increase in occurance of Alternaria alternata, Aspergillus niger, and Aspergillus flavus. Pestaloptiopsis disseminata one of the major pathogen of Som was found highest in aerosphere followed by phyllosphere and it was only dominated in Aheruwa generation. It was found that occurrence of P. disseminata were high when the temperature ranges between 25° - 28° with 70 −80% of relative humidity. This study provides a deep insight into the fungal diversity of host plant Som with respect to pedosphere, aerosphere, and phyllosphere and this knowledge can be used to better select the plantation area and design different disease management strategies to sustain and proliferate the industry for socio-economic development and to conserve its cultural essence.


2018 ◽  
Vol 10 (2) ◽  
pp. 45-54
Author(s):  
MD Hossain ◽  
M Imran ◽  
KA Bhuiyan ◽  
ME Habib ◽  
M Ikrum

A total of 20 chickpea seed samples were collected from BARI, Gazipurand different locations of Savar, Dhaka district. Blotter method was used for detection of the associated fungi of chickpea seeds. Altogether 14 fungi comprising 12 genera namely Aspergillus flavus, Aspergillus niger, Aspergillus sp, Botryodiplodia theobromae, Botryti cinerea, Curvularia lunata, Chaetomium globosum, Cladosporium sp., Colletotrichum dematium, Fusarium oxysporum, Macrophomina phasaelina, Rhizoctonia solani, Rhizopus stolonifer and Stemphylium sarciniforme were isolated from chickpea seed samples. Among the fungi isolated fungi, F. oxysporumwas most prevalent with an average incidence of 18.95% and found in all the seed samples. The germination of seed samples varied from 55-90 % on blotter. The pathogenic fungi and other storage fungi like Aspergillus caused lower the germination of the seeds.A pathogenicity test was conducted with 20 isolates of F. oxysporum against their origin of chickpea seed samples in pot culture. The pathogenecity ranged from weak to highly pathogenic. The isolates FO 19, FO 17, FO 11 and FO 18 were highly virulent. The isolates FO 9, FO10 and FO 15 were virulent and rest of the isolates were moderately virulent. The isolates FO 2 and FO 3 were weak pathogen.J. Environ. Sci. & Natural Resources, 10(2): 45-54 2017


Author(s):  
S. Apuri ◽  
C. Kwoseh ◽  
E. A. Seweh ◽  
I. O. Tutu

Aims: The study aims to identifying the microorganisms associated with post-harvest rot of frafra potatoes in Bongo-soe, Upper east region of Ghana. Place and Duration of Study: Department of Horticulture and the Pathology laboratory of the Faculty of Agriculture, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana. The Solenostemon rotundifolius tubers were stored at the Horticulture Department laboratory whiles rot identification was carried out at the Pathology laboratory of the Faculty of Agriculture. The Tubers were stored from 2nd November, 2012 to 22nd March 2013. Methodology: Four hundred (400) tubers of black cultivar and four hundred (400) tubers of a brown cultivar of Solenostemon rotundifolius tubers showing visible signs of rot during the storage were collected. Pieces of diseased tissues from the margin of the necrotic collected and immersed in 10% commercial bleach solution for sterilisation, for one minute. These were then blotted dry and plated on Potato Dextrose Agar PDA. The plates were sealed with a cellotape until growth occurred. Results: The microorganisms identified to be responsible for causing rot in Solenostemon rotundifolius tubers were six in number. Colletotrichum gloeosporioides was identified to be responsible for 30.76% of rots observed, followed by Aspergillus niger, 23.07%, Curvularia lunata, 19.23%, Aspergillus flavus, 11.54%, Trichoderma sp and Penicillium sp both recorded 7.70% of rots observed. The percentage incidence of Aspergillus niger (15.38%), Curvularia lunata (11.54%) and Aspergillus flavus (7.69%) was higher in the black cultivar as compared with the brown cultivar which had percentage incidence of 7.69%, 7.69% and 3.85% respectively. Also, the percentage incidence of Colletotrichum gloeosporioides (15.38%) and Penicillium sp (3.85%) was the same in both the black and brown cultivars of Solenostemon rotundifolius tubers used in this study. Conclusion: The activities of the damaging microorganisms can be reduced by controlling mechanical injury during harvesting, transportation and storage of Solenostemon rotundifolius tubers should be prevented or reduced because they pave the way for tuber infection by the rot causing microorganisms.


Author(s):  
A. C. Amadioha ◽  
Kenkwo Promise Chidi

Histological studies were carried out to investigate the impact of some pathogenic organisms on infected cassava root after different periods of incubation. Cassava roots inoculated with Aspergillus niger, Rhizopus stolonifer, Aspergillus flavus, and Trichoderma viride showed progressive depletion of the starch grains in the cells and tissue maceration of the cassava root as the period of incubation increased. Cassava root inoculated with A. flavus showed tissue necrosis and slight fragmentation of the starch grains whereas, cassava tissues infected with A. niger showed tissue necrosis and general maceration of the starch grains within the tissue thereby creating empty spaces within the cells with no clear arrangement of the starch grains. Inoculation of the cassava tissues with R. stolonifer resulted in rapid loss of the starch grains and erosion of surrounding cell boundaries. Cassava tissue colonized by T. viride showed tissue necrosis and maceration of the starch grains. Healthy (uninoculated) cassava root tissue showed intact cells packed with large starch grains suggesting that in the host-parasite relationship, the pathogens established themselves inter and intracellularly in the parenchymatous cells of the cortex of the cassava root clearing them of starch grains, and resulting to poor yield and nutritional value of cassava and also economic loss.


2020 ◽  
Vol 14 (4) ◽  
pp. 1282-1293
Author(s):  
Issa Adamou ◽  
Zakari Abdoul Habou ◽  
Moussa S. Hassimi ◽  
Salamatou M. Mamane ◽  
Toudou Adam

Les huiles de Jatropha curcas communément appelé pourghère et de neem (Azadirachta indica A. Juss), sont testées comme alternatives aux pesticides chimiques dans l’optique d’y déceler de potentiels effets fongicides tout en préservant l’environnement. Les effets des deux huiles végétales sont comparés à ceux de deux fongicides conventionnels, la griséofulvine 250 mg et la nystatine ainsi que l’alcool 95°, sur cinq champignons phytopathogènes (Aspergillus flavus, Aspergillus niger, Curvularia lunata, Fusarium oxysporum et Rhizopus oryzae). Les observations ont porté sur la mesure du diamètre de la zone d’inhibition exercée par les différentes substances inhibitrices à travers les milieux gélosés. Le dispositif expérimental appliqué a été celui du bloc complètement randomisé de Fisher avec huit répétitions. Les moyennes des diamètres de la zone d’inhibition sont respectivement de 26,51 mm pour la nystatine, 14,53 mm pour la griséofulvine, 4,53 mm pour l’huile de neem, 3,05 mm pour l’huile de jatropha, et 0,00 mm pour l’alcool 95°. L’huile de neem a eu un effet inhibiteur net sur la croissance de l’ensemble des champignons testés. En revanche, l’huile de jatropha a exercé un meilleur contrôle de la croissance des espèces Aspergillus flavus, Aspergillus niger et Rhizopus oryzae. Les deux huiles pourraient être recommandées dans la protection des denrées stockées, notamment les céréales.Mots clés : Extraits végétaux, effet fongicide, zone d’inhibition, lutte alternative. English Title: Effect of Jatropha (Jatropha curcas L.) and neem (Azadirachta indica A. Juss) oils on in vitro devloppement of pathogenic funji Aspergillus flavus, Aspergillus niger, Curvularia lunata, Fusarium oxysporum et Rhizopus oryzae Oils of Jatropha curcas (pourghère) and neem (Azadirachta indica A. Juss) were tested as alternatives to chemical pesticides in a view to finding some potential fungicide effects harmless to environment. The effects of the two natural oils were compared with those of two conventional fungicides, griseofulvin 250 mg and nystatin and also alcohol 95° on five phytopathogenic fungi (Aspergillus flavus, Aspergillus niger, Curvularia lunata, Fusarium oxysporum and Rhizopus oryzae). Observations consisted in measuring the diameter of the zone of inhibition by different substances through the agarose medium. The experimental device applied was the Fisher completely randomized bloc with eight repetitions. Means of inhibition zone diameters were respectively de 26.51 mm for nystatin, 14.53 mm for griseofulvin, 4.53 mm for neem oil, 3.05 mm for jatropha oil, and 0.00 mm for alcohol 95°. The neem oil showed good inhibition effect on the growth of the whole set of tested fungi. In contrast, jatropha oil exhibited a better fungal growth control on Aspergillus flavus, Aspergillus niger and Rhizopus oryzae species. Thus, these two vegetable oils could be recommended in cereal protection in postharvest.Keywords: Vegetal oils, fungicide effects, inhibition zone, alternative control.


2018 ◽  
Vol 47 (3) ◽  
pp. 389-395
Author(s):  
Resuana Binte Helal ◽  
Sarowar Hosen ◽  
Shamim Shamsi

Nineteen species of fungi namely, Alternaria alternata (Fr.) Keissler, Aspergillus flavus Link, A. fumigatus Fresineus, A. niger Van. Tieghhm, Colletotrichum dematium (Pers.) ex. Fr., C. gloeosporioides (Penz.) Sacc., Corynespora citricola M.B. Ellis, Curvularia lunata Wakker, Fusarium flocciferum Corda, F. nivale (Fr.) Ces., Fusarium Link, Lasiodiplodia theobromae (Pat.) Griff & Moubl, Monilia Pers., Mucor Fresen, Penicillium Link, Pestalotiopsis guepinii (Desm.) Stay., Rhizoctonia solani J.G. Kuhn, Rhizopus stolonifer Bull. and Syncephalastrum Schroet were found to be associated with the diseased fruits of Carica papaya L. Among the isolated fungi C. gloeosporioides, F. nivale and Fusarium sp. were found to be pathogenic for both red and yellow cultivar Shahi papaya. Association of C. lunata, F. nivale, P. guepinii and Syncephalastrum sp. with papaya is a new record. The present report is the first record of Corynespora citricola and Fusarium flocciferum from Bangladesh.


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