Efficient protocols for in vitro axillary bud proliferation and somatic embryogenesis of the medicinal plant Anemopsis californica

2012 ◽  
Vol 6 (22) ◽  
Author(s):  
Araceli Rodríguez-Sahagún
Keyword(s):  
Somatic Embryogenesis ◽  
Medicinal Plant ◽  
Axillary Bud ◽  
Axillary Bud Proliferation ◽  
Anemopsis Californica

scholarly journals Regeneration of Pruatjan (Pimpinella pruatjan Molk): Axillary Bud Proliferation and Encapsulation

2016 ◽  
Vol 2 (2) ◽  
pp. 68 ◽  
Author(s):  
Ika Roostika ◽  
Ireng Darwati ◽  
Ika Mariska
Keyword(s):  
Axillary Bud ◽  
Growth Chamber ◽  
Axillary Bud Proliferation

<p class="p1">Purwoceng (<em>Pimpinella alpina </em>KDS atau <em>Pimpinella pruatjan </em>Molk.) merupakan tanaman obat asli Indonesia yang terancam punah. Akarnya dapat dimanfaatkan sebagai obat afrodisiak, diuretik, dan tonik. Teknik kultur <em>in vitro </em>merupakan teknologi alternatif yang dapat diterapkan untuk konservasi dan perbanyakan tanaman tersebut. Mikropropagasi telah dilakukan melalui jalur organogenesis dengan proliferasi tunas aksilar dan enkapsulasi. Penelitian dilakukan di Laboratorium Kultur Jaringan BB-Biogen, Bogor mulai tahun 2004 hingga 2005. Penelitian ini terbagi atas empat percobaan, yaitu (1) optimasi lingkungan tumbuh kultur, (2) optimasi formulasi media untuk proliferasi tunas aksilar dan enkapsulasi tunas aksilar, (3) induksi perakaran, dan (4) aklimatisasi. Kondisi lingkungan kultur yang optimum adalah di <em>growth chamber </em>dengan suhu 9<span class="s1">o</span>C dan intensitas cahaya 1000 lux. Formulasi media terbaik untuk proliferasi tunas aksilar adalah media DKW dengan penambahan BA 4 ppm dengan eksplan berupa tunas tanpa daun. Penggunaan arginin 100 ppm lebih baik daripada glutamin 100 ppm dan modifikasi vitamin (mioinositol 100 ppm dan thiamine-HCl 1 ppm). Pada media yang sama, pertumbuhan tunas aksilar terenkapsulasi juga paling baik dan tunas tersebut dapat menembus kapsul alginat setelah 4 minggu dalam periode <em>in vitro </em>(85%). Penggunaan NAA 1,0 ppm menginduksi perakaran paling cepat (40 hari) dengan persentase perakaran paling tinggi (100%). Vermikulit bertekstur kasar paling baik untuk aklimatisasi tunas aksilar terenkapsulasi sedangkan arang sekam paling baik untuk aklimatisasi planlet.</p>

Rapid Propagation System in Vitro of Medicinal Plant Cynanchum Atratum Bunge

2021 ◽  
Author(s):  
P Chang ◽  
GF Dong ◽  
MF Li ◽  
YH Zhang ◽  
Yumei Dong
Keyword(s):  
Urinary Infection ◽  
Axillary Bud ◽  
Adventitious Bud ◽  
Ms Medium ◽  
Adventitious Buds ◽  
Axillary Bud Proliferation ◽  
Rapid Propagation ◽  
Wild Resources ◽  
Culture Technology

Abstract As a traditional Chinese medicinal material, Cynanchum atratum Bunge has been widely used in traditional Chinese medicine for its treatment of abscesses, acute urinary infection and hectic fever.Thus, wild resources of it have been endangered by overharvesting. Plant tissue culture technology is an important measure to protect wild resources of medicinal plants, including C. atratum. Therefore, a fast and efficient propagation system of C. atratum through axillary bud proliferation pathwayhas been established. Through axillary bud proliferation, the medium [MS+sucrose 30 g/L+Agar 7 g/L+NAA 0.2 mg/l+IBA 1.5mg/l+KT 0.5 mg/l] can effectively proliferate adventitious buds, and the induction rate was 100 %, proliferation coefficient could reach 8.56. MS medium was used to induce adventitious bud rooting, with rooting rate of 98% and no callus. The highest survival rate was 90% when the ratio of grass mud pond and orchard red soil was 1:1. To our knowledge this is the first report of rapid propagation system in C. atratum, it achieve rapid reproduction of C. atratum.

In vitro propagation of the Chinese traditional and medicinal plant Heracleum scabridum Franch

2019 ◽  
Vol 48 (3) ◽  
pp. 575-581
Author(s):  
Li-Juan Zou ◽  
Jin-Yao Hu ◽  
Ming-Hua Luo ◽  
Qing-Gui Wu
Keyword(s):  
Medicinal Plant ◽  
Shoot Regeneration ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Axillary Bud ◽  
Direct Organogenesis ◽  
Ms Medium ◽  
Indirect Organogenesis ◽  
Best Response

Three explants such as stem tips, leaves and petioles of Heracleum scabridum were compared for their shoot development/differentiation ability by using different plant growth regulators (PGRs). The most effective PGRs combination for callus induction and organogenesis was determined. TDZ, Kn, Zn and IAA were used to induce multiple shoots. For indirect organogenesis (from the calli), the best response (27.25 ± 4.19 shoot per stem tips) and (18.23 ± 2.12 per leaves) were obtained in Murashige and Skoog (MS) medium fortified with 0.5 mg/l IAA and 3.0 mg/l Zn with 100, 97.3% induction rate, respectively. MS medium containing 0.5 mg/l IAA and 3.0 mg/l Zn was also found to be optimal for shoot regeneration from petioles. The highest percentage of regeneration (94.6) with mean number of shoots 17.83 ± 4.87 from petioles were produced. For direct organogenesis (from axillary bud shoot clumps), 0.1 mg/l IAA and 1.5 mg/l TDZ were found to be optimal for shoot regeneration of stem tips, with mean numbers of axillary bud shoot clumps 7.12 ± 1.23 were produced. Plantlets were transferred to soil with 95% of plants acclimatized recovered successfully.  

scholarly journals Efficient in vitro Regeneration of a Medicinal Plant Harsinghar (Nyctanthes arbor-tristis L.)

2013 ◽  
Vol 22 (2) ◽  
pp. 137-142 ◽  
Author(s):  
S Bansal ◽  
AJ Bharati ◽  
YK Bansal
Keyword(s):  
Medicinal Plant ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Axillary Bud ◽  
Root Initiation ◽  
Maximum Frequency ◽  
Half Strength

In vitro propagation of Nyctanthes arbor-tristis L. has been successfully established from axillary bud explants on MS. Maximum number of multiple shoots was obtained on MS containing BAP (22.2 ?M). Half strength of MS (2% sucrose) supplemented with NAA (10.74 ?M) provided the maximum frequency of root initiation. The plantlets were successfully hardened. DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14202 Plant Tissue Cult. & Biotech. 22(2): 137-142, 2012 (December)

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