scholarly journals <i>In vitro</i> propagation of <i>Aegle marmelos</i> (L.) corr., a medicinal plant through axillary bud multiplication

2012 ◽  
Vol 03 (02) ◽  
pp. 121-125 ◽  
Author(s):  
Puspashree Puhan ◽  
Shiba Prasad Rath
Phytomedicine ◽  
2003 ◽  
Vol 10 (4) ◽  
pp. 300-308 ◽  
Author(s):  
I. Lampronti ◽  
D. Martello ◽  
N. Bianchi ◽  
M. Borgatti ◽  
E. Lambertini ◽  
...  

2019 ◽  
Vol 48 (3) ◽  
pp. 575-581
Author(s):  
Li-Juan Zou ◽  
Jin-Yao Hu ◽  
Ming-Hua Luo ◽  
Qing-Gui Wu

Three explants such as stem tips, leaves and petioles of Heracleum scabridum were compared for their shoot development/differentiation ability by using different plant growth regulators (PGRs). The most effective PGRs combination for callus induction and organogenesis was determined. TDZ, Kn, Zn and IAA were used to induce multiple shoots. For indirect organogenesis (from the calli), the best response (27.25 ± 4.19 shoot per stem tips) and (18.23 ± 2.12 per leaves) were obtained in Murashige and Skoog (MS) medium fortified with 0.5 mg/l IAA and 3.0 mg/l Zn with 100, 97.3% induction rate, respectively. MS medium containing 0.5 mg/l IAA and 3.0 mg/l Zn was also found to be optimal for shoot regeneration from petioles. The highest percentage of regeneration (94.6) with mean number of shoots 17.83 ± 4.87 from petioles were produced. For direct organogenesis (from axillary bud shoot clumps), 0.1 mg/l IAA and 1.5 mg/l TDZ were found to be optimal for shoot regeneration of stem tips, with mean numbers of axillary bud shoot clumps 7.12 ± 1.23 were produced. Plantlets were transferred to soil with 95% of plants acclimatized recovered successfully.  


2013 ◽  
Vol 22 (2) ◽  
pp. 137-142 ◽  
Author(s):  
S Bansal ◽  
AJ Bharati ◽  
YK Bansal

In vitro propagation of Nyctanthes arbor-tristis L. has been successfully established from axillary bud explants on MS. Maximum number of multiple shoots was obtained on MS containing BAP (22.2 ?M). Half strength of MS (2% sucrose) supplemented with NAA (10.74 ?M) provided the maximum frequency of root initiation. The plantlets were successfully hardened. DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14202 Plant Tissue Cult. & Biotech. 22(2): 137-142, 2012 (December)


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