Thidiazuron induced high frequency axillary shoot multiplication in Withania somnifera L. Dunal

2011 ◽  
Vol 5 (30) ◽  
Author(s):  
Fatima N
Keyword(s):  
High Frequency ◽  
Withania Somnifera ◽  
Shoot Multiplication ◽  
Axillary Shoot ◽  
Axillary Shoot Multiplication

scholarly journals Micropropagation of Rhododendron chapmanii

1986 ◽  
Vol 4 (1) ◽  
pp. 26-29
Author(s):  
Frank A. Blazich ◽  
Cheryl G. Giles ◽  
Carole M. Haemmerle
Keyword(s):  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Axillary Shoot ◽  
Axillary Shoots ◽  
Light Levels ◽  
Axillary Shoot Multiplication ◽  
Reduced Light

Shoot tips excised from Chapman's rhododendron (Rhododendron chapmanii A. Gray) were surface sterilized, the terminal portions were removed (decapitated) and the shoots placed in liquid Woody Plant Medium (WPM) supplemented with 8 μM (1.6 ppm) 6(γ, γ-dimethylallylamino)-purine (2iP). Within 2 to 3 months axillary shoots excised, decapitated and cultured on agarsolidified WPM supplemented with 49 μM (10 ppm) 2iP. Multiple shoot formation consisting of adventitious and axillary shoots was observed within 4 to 6 months. These shoots were transferred to WPM supplemented With 8 μM (1.6 ppm) 2iP and cultured under reduced light levels to stimulate shoot elongation. Shoots ≥ 10 mm (0.4 in.) were harvested (microcuttings) and rooted using non-in vitro procedures. Enhancement of axillary shoot multiplication was achieved by culturing decapitated axillary shoots under reduced light levels in a horizontal position on WPM supplemented WIth 8 μM (1.6 ppm) 2iP.

scholarly journals Optimization of media formulation for axillary shoot multiplication of the red-peeled sweet potato (Ipomoea batatas [L.] Lam.) ‘Abees’

2020 ◽  
Vol 80 (1) ◽  
pp. 3-10
Author(s):  
Yaser Hassan Dewir ◽  
Abdulhakim A. Aldubai ◽  
Mafatlal M. Kher ◽  
Abdullah A. Alsadon ◽  
Salah El-Hendawy ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Shoot Multiplication ◽  
Axillary Shoot ◽  
Media Formulation ◽  
Axillary Shoot Multiplication

scholarly journals Micropropagation of Flame Azalea

1988 ◽  
Vol 6 (2) ◽  
pp. 45-47
Author(s):  
Frank A. Blazich ◽  
Juan R. Acedo
Keyword(s):  
Woody Plant ◽  
Shoot Multiplication ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Axillary Shoot ◽  
Stock Plant ◽  
Axillary Shoots ◽  
Growing Stock ◽  
Woody Plant Medium ◽  
Axillary Shoot Multiplication

Shoots tips excised from an actively growing stock plant of flame azalea [Rhododendron calendulaceum (Michx.) Torr.] were surface sterilized, the terminal portions were removed (decapitated) and the shoots placed horizontally on agar-solidified Woody Plant Medium (WPM) supplemented with 15 ppm 6-(γ, γ-dimethylallylamino)-purine (2iP). Within 4 to 6 months multiple shoot formation commenced. After 2 to 3 additional months of growth, axillary shoots were excised from the original explants. The shoots were decapitated and placed on WPM. After 2 subcultures, 8-node axillary shoots were excised, decapitated and cultured on agar-solidified WPM supplemented with 0, 4, 8, 12, 16, 24, and 32 ppm 2iP. The greatest number of shoots (microcuttings) ≥ 5 mm (0.2 in) were produced at 12 ppm 2iP. Microcuttings ≥ 10 mm (0.4 in) were rooted using ex vitro procedures. Enhancement of both axillary shoot multiplication and shoot length was achieved by addition to the medium of 80 ppm adenine sulfate and 200 ppm NaH2PO4.

scholarly journals In vitro Mass Propagation of a Ground Orchid - Phaius tancarvilleae (L'Her.) Blume through Shoot Tip Culture

2011 ◽  
Vol 21 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Bijaya Pant ◽  
Sumitra Shrestha
Keyword(s):  
High Frequency ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Shoot Tip ◽  
Mass Propagation ◽  
Shoot Tip Culture ◽  
Shoot Tip Explants ◽  
Direct Shoot

High frequency direct shoot proliferation was induced from the shoot tip explants derived from the in vitro grown seedlings of a critically endangered and horticulturally important ground orchid Phaius tancarvilleae (L'Her) Blume. Shoot tip explants cultured on solidified MS with alone or combination of various concentrations of NAA and BAP produced shoots and multiple shoots. The maximum number of healthy shoots was observed on MS with BAP (1.0 mg/l) with an average of 13.3 shoots per culture in 20 weeks; where shoot multiplication was initiated after 4 weeks of culture. Regenerated shoots rooted on MS with various concentrations of NAA, IAA, IBA. MS with NAA (0.5 mg/l) was the most appropriate condition for rooting. The well developed in vitro rooted plantlets were hardened successfully in the potting mixture containing cocopeat and sphagnum moss in the ratio of 2 : 1.   Key words: Mass propagation, Phaius tancarvilleae, shoot multiplication   D. O. I. 10.3329/ptcb.v21i2.10241   Plant Tissue Cult. & Biotech. 21(2): 181-188, 2011 (December)

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