Effect of Explant Source on Axillary Shoot Multiplication During Micropropagation of a Rare Medicinal Plant — Wattakaka volubilis (L f) Stapf

2006 ◽  
Vol 15 (1) ◽  
pp. 43-45 ◽  
Author(s):  
T. Chakradhar ◽  
T. Pullaiah
Keyword(s):  
Medicinal Plant ◽  
Shoot Multiplication ◽  
Axillary Shoot ◽  
Explant Source ◽  
Axillary Shoot Multiplication

scholarly journals In Vitro Regeneration of Vitex negundo L., a Woody Valuable Medicinal Plant through High Frequency Axillary Shoot Proliferation

1970 ◽  
Vol 43 (3) ◽  
pp. 345-352 ◽  
Author(s):  
Farhana Afroz ◽  
AKM Sayeed Hassan ◽  
Laila Shamroze Bari ◽  
Rebeka Sultana ◽  
John Liton Munshi ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Large Scale ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Nodal Segments ◽  
Axillary Shoot ◽  
Vitex Negundo ◽  
Axillary Shoot Proliferation

An efficient protocol was established for rapid and large scale propagation of woody aromatic medicinal plant Vitex negundo L. by in vitro shoot multiplication from shoot tips and nodal segments of mature plant. Of the four different growth regulators BA, Kn, GA3, NAA and coconut water, MS fortified with BA 1.0 mg/l was found to be the most effective for inducing multiple shoots from nodal explants. The percentage (96%) of shoot multiplication per node (21.83) was highest up to second subculture passages, after which there was a gradual decline in shoot development. Best rooting was induced (93%) in excised shoots on half strength MS medium supplemented with an optimal combination of NAA (0.3 mg/l). Soil, compost and sand (1:1:1) mixture was the most suitable planting substrate for hardening. The survival rate was 80% and the regenerated plants were successfully transferred to the soil.Key words: Vitex negundo, Medicinal plant, Shoot proliferation, Micropropagation, RegenerationDOI = 10.3329/bjsir.v43i3.1149Bangladesh J. Sci. Ind. Res. 43(3), 345-352, 2008

scholarly journals Micropropagation of Rhododendron chapmanii

1986 ◽  
Vol 4 (1) ◽  
pp. 26-29
Author(s):  
Frank A. Blazich ◽  
Cheryl G. Giles ◽  
Carole M. Haemmerle
Keyword(s):  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Axillary Shoot ◽  
Axillary Shoots ◽  
Light Levels ◽  
Axillary Shoot Multiplication ◽  
Reduced Light

Shoot tips excised from Chapman's rhododendron (Rhododendron chapmanii A. Gray) were surface sterilized, the terminal portions were removed (decapitated) and the shoots placed in liquid Woody Plant Medium (WPM) supplemented with 8 μM (1.6 ppm) 6(γ, γ-dimethylallylamino)-purine (2iP). Within 2 to 3 months axillary shoots excised, decapitated and cultured on agarsolidified WPM supplemented with 49 μM (10 ppm) 2iP. Multiple shoot formation consisting of adventitious and axillary shoots was observed within 4 to 6 months. These shoots were transferred to WPM supplemented With 8 μM (1.6 ppm) 2iP and cultured under reduced light levels to stimulate shoot elongation. Shoots ≥ 10 mm (0.4 in.) were harvested (microcuttings) and rooted using non-in vitro procedures. Enhancement of axillary shoot multiplication was achieved by culturing decapitated axillary shoots under reduced light levels in a horizontal position on WPM supplemented WIth 8 μM (1.6 ppm) 2iP.

scholarly journals In vitro Regeneration through Apical and Axillary Shoot Proliferation of Ficus religiosa L. - A Multi-purpose Woody Medicinal Plant

2010 ◽  
Vol 19 (1) ◽  
pp. 71-78 ◽  
Author(s):  
A.K.M. Sayeed Hassan ◽  
Farhana Afroz ◽  
Miskat Ara Akhter Jahan ◽  
Rahima Khatun
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Axillary Shoot ◽  
Axillary Shoot Proliferation ◽  
Ficus Religiosa ◽  
Half Strength

A protocol was established for mass propagation of the valuable medicinal plant Ficus religiosa L. (Moraceae) through in vitro culture using apical and axillary buds of young sprouts from selected plants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP + 0.1 mg/l IAA, in which 78 per cent of the explants produced 16 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 24 shoots per culture. In vitro raised shoots rooted on half strength MS supplemented with 2.0 mg/l IBA + 0.1 mg/l NAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for seven days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85 per cent.  Key words: Ficus religiosa, Medicinal plant, Shoot proliferation, Regeneration,                   Acclimatization D.O.I. 10.3329/ptcb.v19i1.4987 Plant Tissue Cult. & Biotech. 19(1): 71-78, 2009 (June)

scholarly journals In vitro Shoot Multiplication of Bupleurum disticho-phyllum Wight - A Native Medicinal Plant of Southern India

1970 ◽  
Vol 17 (2) ◽  
pp. 115-124 ◽  
Author(s):  
S. Karuppusamy ◽  
T. Pullaiah
Keyword(s):  
Medicinal Plant ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Formation ◽  
Shoot Tip ◽  
Axillary Shoot ◽  
Axillary Shoots ◽  
Axillary Shoot Proliferation ◽  
Shoot Tip Explants

Shoot multiplication of Bupleurum distichophyllum was achieved from the nodal and shoot tip explants of mature plants using MS with different concentrations and combinations of growth regulators. Maximum explant response was from axillary shoots and the highest number of shoots per explant was obtained on MS fortified with 1.0 mg/l BAP. The highest degree of axillary shoot proliferation was found to be 74 and 70% for nodal- and shoot tip explants, respectively on the medium containing 1.0 mg/l BAP + 0.1 mg/l NAA. The combination of BAP and GA3 was also found to be effective for both type of explants. The degree of shoot formation was affected by explant types and the exogenous hormonal regime in the medium. The regenerated shoots were successfully rooted on MS supplemented with 2.0 mg/l IBA, after sequential hardening, survival rate was 71%. Key words: Bupleurum distichophyllum, Medicinal plant, Micropropagation, Conservation Plant Tissue Cult. & Biotech. 17(2): 115-124, 2007 (December) DOI: 10.3329/ptcb.v17i2.2574

scholarly journals Optimization of media formulation for axillary shoot multiplication of the red-peeled sweet potato (Ipomoea batatas [L.] Lam.) ‘Abees’

2020 ◽  
Vol 80 (1) ◽  
pp. 3-10
Author(s):  
Yaser Hassan Dewir ◽  
Abdulhakim A. Aldubai ◽  
Mafatlal M. Kher ◽  
Abdullah A. Alsadon ◽  
Salah El-Hendawy ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Shoot Multiplication ◽  
Axillary Shoot ◽  
Media Formulation ◽  
Axillary Shoot Multiplication

scholarly journals Micropropagation of Flame Azalea

1988 ◽  
Vol 6 (2) ◽  
pp. 45-47
Author(s):  
Frank A. Blazich ◽  
Juan R. Acedo
Keyword(s):  
Woody Plant ◽  
Shoot Multiplication ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Axillary Shoot ◽  
Stock Plant ◽  
Axillary Shoots ◽  
Growing Stock ◽  
Woody Plant Medium ◽  
Axillary Shoot Multiplication

Shoots tips excised from an actively growing stock plant of flame azalea [Rhododendron calendulaceum (Michx.) Torr.] were surface sterilized, the terminal portions were removed (decapitated) and the shoots placed horizontally on agar-solidified Woody Plant Medium (WPM) supplemented with 15 ppm 6-(γ, γ-dimethylallylamino)-purine (2iP). Within 4 to 6 months multiple shoot formation commenced. After 2 to 3 additional months of growth, axillary shoots were excised from the original explants. The shoots were decapitated and placed on WPM. After 2 subcultures, 8-node axillary shoots were excised, decapitated and cultured on agar-solidified WPM supplemented with 0, 4, 8, 12, 16, 24, and 32 ppm 2iP. The greatest number of shoots (microcuttings) ≥ 5 mm (0.2 in) were produced at 12 ppm 2iP. Microcuttings ≥ 10 mm (0.4 in) were rooted using ex vitro procedures. Enhancement of both axillary shoot multiplication and shoot length was achieved by addition to the medium of 80 ppm adenine sulfate and 200 ppm NaH2PO4.

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