scholarly journals Evaluation of radical scavenging activity of rhizome extracts of Alpinia galanga and Zingiber officinale

2012 ◽  
Vol 6 (41) ◽  
pp. 5406-5411 ◽  
Author(s):  
Karuppiah Pillai Manoharan ◽  
Zaini Asmawi Mohd ◽  
Soo Choon Tan ◽  
Sasidharan Sreenivasan ◽  
Jothy Lachumy Subramanion ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Zingiber Officinale ◽  
Scavenging Activity ◽  
Alpinia Galanga

scholarly journals Penentuan Aktivitas Antioksidan Senyawa Kuersetin dan Ekstrak Lengkuas Menggunakan HPLC dan UV-Vis

ALCHEMY ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 24-32
Author(s):  
Bambang Cahyono ◽  
Christiana Suci Prihatini ◽  
Meiny Suzery ◽  
Damar Nurwahyu Bima
Keyword(s):  
Antioxidant Activity ◽  
Spectrophotometric Method ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Hplc Method ◽  
Scavenging Activity ◽  
Alpinia Galanga ◽  
Radical Reduction ◽  
Alpinia Purpurata ◽  
Better Than

The radical scavenging activity of 1,1-diphenyl-2-picrylhidrazyl (DPPH) is generally carried out using a spectrophotometric method. In this study, the value of the antioxidant activity was compared to the HPLC method. Samples used were pure quercetin and extracts of red galangal (Alpinia purpurata) and white galangal (Alpinia galanga). Analysis of antioxidant activity using a UV/Vis spectrophotometer was carried out at a wavelength of 515 nm. Analysis by HPLC method was carried out using an inverse phase with a UV/Vis detector at 515 nm. The results showed that the radical scavenging activity (IC50) of the pure quercetin produced was nearly the same value for the spectrophotometric method (16.24 ppm) dan the HPLC method (15.24 ppm). Even though, the antioxidant activity of pure quercetin was different from the extract. The red galangal extract gave IC50 of 488.43±1.13 ppm (spectrophotometric method) and IC50 of 68.12±10.19 ppm (HPLC method). The radical scavenging activity (IC50) of white galangal extract using the spectrophotometric method and HPLC method was 462.47±2.98 and 62.17±3.87 ppm, respectively. The allegation of other molecular interference in the radical reduction of the extract resulted in a conclusion that the HPLC is better than the spectrophotometric method for determining antoxidant activity of extract sample. Keywords: Antioxidant, Alpinia purpurata, Alpinia galanga, DPPH  Aktivitas peredaman radikal 1,1-diphenyl-2-picrylhidrazyl (DPPH) pada umumnya dilakukan menggunakan metode spektrofotometer. Dalam penelitian ini, nilai aktivitas antioksidan tersebut telah dibandingkan dengan metode HPLC. Sampel ujinya adalah senyawa kursetin murni dan ekstrak lengkuas merah (Alpinia purpurata) dan lengkuas putih (Alpinia galanga). Analisis aktivitas antioksidan dengan spektrofotometer UV/Vis dilakukan pada panjang gelombang 515 nm. Analisis dengan metode HPLC dilakukan menggunakan fasa inverse dengan detektor UV/Vis pada 515 nm. Hasil penelitian menunjukkan bahwa aktivitas peredaman radikal senyawa murni kuersetin menghasilkan nilai yang hampir sama untuk kedua metode yaitu IC50 sebesar 17,05 ppm untuk metode spektrofotometer dan IC50 sebesar 15,74 ppm untuk metode HPLC. Akan tetapi, nilai aktivitas antioksidan kuersetin jauh berbeda untuk ekstrak. Ekstrak lengkuas merah memberikan IC50 sebesar 488,43±1,13 ppm (metode spektrofotometer) dan 68,12±10,19 ppm (metode HPLC). Aktivitas peredaman radikal ekstrak lengkuas putih dengan metode spektrofotometer dan HPLC dengan IC50 sebesar 462,89±5,38 dan 62,17±3,87 ppm, berturut-turut. Dugaan adanya interferensi molekul lain dalam analisis peredaman radikal terhadap ekstrak ini menghasilkan suatu kesimpulan bahwa metode HPLC lebih baik digunakan dalam analisis antioksidan dibandingkan metode spektrofotometer untuk sampel berupa ekstrak. Kata kunci: Antioksidan, Alpinia purpurata, Alpinia galanga, DPPH

scholarly journals DEVELOPMENT OF HERBAL TEA USING MORINGA OLIFERA, ELSHOLTZIA COMMUNIS AND ALPINIA GALANGA: A SENSORY ACCEPTANCE STUDY

2021 ◽  
Vol 9 (07) ◽  
pp. 446-453
Author(s):  
Chandrama Baruah ◽  
◽  
Yelika Yepthomi ◽  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Market Potential ◽  
Scavenging Activity ◽  
Herbal Tea ◽  
Alpinia Galanga ◽  
Overall Acceptability ◽  
Methanolic Extracts ◽  
Total Antioxidant ◽  
Dpph Scavenging

The present study aims to develop an herbal tea formulation from leaves of Moringa oleifera incorporating with rhizomes of Alpinia galanga and the inflorescence of Elsholtzia communis with an insight to improve its sensory attributes and also enhancing the nutritional value. The ingredients were processed and three herbal teas HT1, HT2, HT3 were formulated by mixing them at different levels of incorporation. Acceptability trials for color, aroma, taste and overall aceptability revealed that HT2 was the most liked infusion with highest scores for color (7.8), taste (8.04) and overall acceptability (7.96). The total antioxidant capacity of HT2 was determined by the DPPH scavenging activity test by the method of Blois. The methanolic extracts of the herbal tea sample showed 76.12% radical scavenging activity whereas the sample extracted with DMSO showed a lower antioxidants capacity of 62.45%. The shelf life of the herbal tea was studied by storing in different packaging materials. HDPE Zip loc bags, tea bags (packed in paper cartons) and HDPE containers stored the product without spoilage over a period of six months. The developed herbal tea has a good market potential with significant health benefits.

scholarly journals Potency of Radical Scavenging Activity and Determination of Total Phenolic Content of Five Ethanolic Extract of Rhizome Zingiberaceae Family

2013 ◽  
Vol 4 (1) ◽  
pp. 457
Author(s):  
Muhammad Da'i ◽  
Didiek Setiawan ◽  
Rosita Melannisa
Keyword(s):  
Phenolic Compound ◽  
Antiradical Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Zingiber Officinale ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Total Phenolic Compound ◽  
Dpph Method

Several studies show that some plants and fruits could protect human body from free radical danger exposure. Zingiberaceae family has some chemical substances that have antiradical activity, such as phenolic compound and flavonoid. The aim of this study is to determine the correlation of total phenolic compound towards its antiradical activity. Antiradical activity assay was determined by DPPH method (1,1 difenil-2-pikrilhidrazil). The total phenolic compound is determined by spectrofotometry using Folin-Ciocalteu reagent and counted as GAE (Gallic Acid Equivalent). Based on this study, the total phenolic concentration of ethanolic extract of jahe (Zingiber officinale (L.) Rosc), kencur (Kaemferia galanga L.), lengkuas (Languas galanga), lempuyang gajah (Zingiber littorale) and lempuyang pahit (Zingiber americana) is 73.99 mg/g; 23.848 mg/g; 37.841 mg/g; 49.725 mg/g and 40.802 mg/g respectively. The radical scavenging activity (IC50) of jahe (27.38 µg/mL), lengkuas (93,27 µg/mL), lempuyang pahit (105,08 µg/mL), meanwhile lempuyang gajah and kencur show low activity of radical scavenging.Keywords: Zingiberaceae, total phenolic compound, antiradical, DPPH

Phenolic constituents of Crithmum maritimum and their radical scavenging activity

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
S Ngom ◽  
L Breant ◽  
C Antheaume ◽  
C Minker ◽  
A Leick ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Phenolic Constituents
Sign in / Sign up

Export Citation Format

Share Document