Ecofriendly management of mixed coconut oil cake waste for lipase production by marine Streptomyces indiaensis and utilization as detergent additive

Author(s):  
B. Sathya Priya
2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Anahita Khoramnia ◽  
Afshin Ebrahimpour ◽  
Boon Kee Beh ◽  
Oi Ming Lai

The lipase production ability of a newly isolatedAcinetobactersp. in submerged (SmF) and solid-state (SSF) fermentations was evaluated. The results demonstrated this strain as one of the rare bacterium, which is able to grow and produce lipase in SSF even more than SmF. Coconut oil cake as a cheap agroindustrial residue was employed as the solid substrate. The lipase production was optimized in both media using artificial neural network. Multilayer normal and full feed forward backpropagation networks were selected to build predictive models to optimize the culture parameters for lipase production in SmF and SSF systems, respectively. The produced models for both systems showed high predictive accuracy where the obtained conditions were close together. The produced enzyme was characterized as a thermotolerant lipase, although the organism was mesophile. The optimum temperature for the enzyme activity was 45°C where 63% of its activity remained at 70°C after 2 h. This lipase remained active after 24 h in a broad range of pH (6–11). The lipase demonstrated strong solvent and detergent tolerance potentials. Therefore, this inexpensive lipase production for such a potent and industrially valuable lipase is promising and of considerable commercial interest for biotechnological applications.


2015 ◽  
Vol 20 (2) ◽  
pp. 125-133 ◽  
Author(s):  
PALANISAMY GOWTHAMI ◽  
KARUPPAN MUTHUKUMAR ◽  
MANICKAM VELAN

Author(s):  
J. Mary Sheela ◽  
K. Divya ◽  
S. Premina

Amylase enzymes are starch degrading enzymes and have received a great deal of attention due to their perceived technology importance and economic benefit. Amylase enzymes are considered important enzymes used in starch processing industries for the hydrolysis of polysaccharides like starch into simple sugar constituents. This enzyme is also involved in the commercial production of glucose. Solid-state cultivation and submerged cultivation have tremendous potentials for enzyme amylase production by using different solid substrates like rice bran, wheat bran, coconut oil cake, and groundnut oil cake which are rich in starch. These agro-industrial wastes are considered cheap raw materials for the production of amylase. Wastewater from the industry like brewery can also be used as a liquid substrate for submerged cultivation. It may have the possibility of depurination of wastewater. In the present study, Aspergillus niger and Penicillium species were isolated and their amylase activity was determined by the starch hydrolysis method. Enzyme production was done by using coconut oil cake as a substrate for solid-state fermentation and brewery wastewater as a substrate for submerged fermentation. The enzyme produced by the organisms was extracted and enzyme assay was done by the Dinitrisalicilic method (DNS method). The protein estimation was done by Lowry Folin’s method. The qualitative assay was carried out by performing Gas Chromatography-Mass Spectroscopy (GC-MS).


2020 ◽  
Vol 50 (6) ◽  
pp. 578-584 ◽  
Author(s):  
Rajesh Kumar Sahoo ◽  
Aradhana Das ◽  
Mahendra Gaur ◽  
Anshuman Sahu ◽  
Saubhagini Sahoo ◽  
...  

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Vidhya Prabhudessai ◽  
Anasuya Ganguly ◽  
Srikanth Mutnuri

The focus of our work is on anaerobic digestion of locally available agro wastes like coconut oil cake, cashew apple waste, and grass from lawn cuttings. The most productive agro waste, in terms of methane yield, was coconut oil cake and grass. The results showed that the initial volatile solids concentration significantly affected the biogas production. The methane yield from coconut oil cake was found to be 383 ml CH4/g VS and 277 ml CH4/g VS added at 4 and 4.5 g VS/l. In case of grass the biogas production increased with increasing VS concentrations with methane yield of 199, 250, 256, 284, and 332 ml CH4/g VS at 3, 3.5, 4, 4.5, and 5.0 g VS/l. For cashew apple waste single-stage fermentation inhibited biogas production. However, phase separation showed methane yield of 60.7 ml CH4/g VS and 64.6 ml CH4/g VS at 3.5 and 4.0 g VS/l, respectively. The anaerobic biodegradability of coconut oil cake was evaluated in fed batch mode in a 5 L anaerobic reactor at 4 g VS/L per batch, and the maximum methane yield was found to be 320 ml CH4/g VS.


2019 ◽  
Vol 42 (4) ◽  
Author(s):  
Puthiya P. Shameena Beegum ◽  
Musuvadi R. Manikantan ◽  
Monika Sharma ◽  
Ravi Pandiselvam ◽  
Ram K. Gupta ◽  
...  

2016 ◽  
Vol 52 (2) ◽  
pp. 540-549
Author(s):  
Shameena Beegum ◽  
Monika Sharma ◽  
Musuvadi Ramarathinam Manikantan ◽  
Ram Kishor Gupta

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