scholarly journals Production of a Solvent, Detergent, and Thermotolerant Lipase by a Newly IsolatedAcinetobactersp. in Submerged and Solid-State Fermentations

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Anahita Khoramnia ◽  
Afshin Ebrahimpour ◽  
Boon Kee Beh ◽  
Oi Ming Lai
Keyword(s):  
Solid State ◽  
Predictive Models ◽  
Predictive Accuracy ◽  
Coconut Oil ◽  
Lipase Production ◽  
Solid Substrate ◽  
Biotechnological Applications ◽  
Optimum Temperature ◽  
Agroindustrial Residue ◽  
Coconut Oil Cake

The lipase production ability of a newly isolatedAcinetobactersp. in submerged (SmF) and solid-state (SSF) fermentations was evaluated. The results demonstrated this strain as one of the rare bacterium, which is able to grow and produce lipase in SSF even more than SmF. Coconut oil cake as a cheap agroindustrial residue was employed as the solid substrate. The lipase production was optimized in both media using artificial neural network. Multilayer normal and full feed forward backpropagation networks were selected to build predictive models to optimize the culture parameters for lipase production in SmF and SSF systems, respectively. The produced models for both systems showed high predictive accuracy where the obtained conditions were close together. The produced enzyme was characterized as a thermotolerant lipase, although the organism was mesophile. The optimum temperature for the enzyme activity was 45°C where 63% of its activity remained at 70°C after 2 h. This lipase remained active after 24 h in a broad range of pH (6–11). The lipase demonstrated strong solvent and detergent tolerance potentials. Therefore, this inexpensive lipase production for such a potent and industrially valuable lipase is promising and of considerable commercial interest for biotechnological applications.

scholarly journals Amylase Production by Aspergillus niger and Penicillium Species by Solid-State and Submerged Cultivation Using Two Food Industrial Wastes

2021 ◽  
Vol 20 (3) ◽  
Author(s):  
J. Mary Sheela ◽  
K. Divya ◽  
S. Premina
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Coconut Oil ◽  
Industrial Wastes ◽  
Submerged Cultivation ◽  
Brewery Wastewater ◽  
Groundnut Oil ◽  
Penicillium Species ◽  
Amylase Production ◽  
Coconut Oil Cake

Amylase enzymes are starch degrading enzymes and have received a great deal of attention due to their perceived technology importance and economic benefit. Amylase enzymes are considered important enzymes used in starch processing industries for the hydrolysis of polysaccharides like starch into simple sugar constituents. This enzyme is also involved in the commercial production of glucose. Solid-state cultivation and submerged cultivation have tremendous potentials for enzyme amylase production by using different solid substrates like rice bran, wheat bran, coconut oil cake, and groundnut oil cake which are rich in starch. These agro-industrial wastes are considered cheap raw materials for the production of amylase. Wastewater from the industry like brewery can also be used as a liquid substrate for submerged cultivation. It may have the possibility of depurination of wastewater. In the present study, Aspergillus niger and Penicillium species were isolated and their amylase activity was determined by the starch hydrolysis method. Enzyme production was done by using coconut oil cake as a substrate for solid-state fermentation and brewery wastewater as a substrate for submerged fermentation. The enzyme produced by the organisms was extracted and enzyme assay was done by the Dinitrisalicilic method (DNS method). The protein estimation was done by Lowry Folin’s method. The qualitative assay was carried out by performing Gas Chromatography-Mass Spectroscopy (GC-MS).

scholarly journals Solid State Fermentation of Aspergillus oryzae for Glucoamylase Production on Agro residues

2010 ◽  
Vol 4 ◽  
pp. 16-25 ◽  
Author(s):  
Vasudeo Zambare
Keyword(s):  
Solid State ◽  
Aspergillus Oryzae ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Coconut Oil ◽  
Groundnut Oil ◽  
Amylolytic Enzyme ◽  
Coconut Oil Cake ◽  
Agro Residues ◽  
Glucoamylase Production

Glucoamylase is a well recognized amylolytic enzyme used in food industry, which is generally produced by Aspergillus genus under solid-state fermentation (SSF). This study presents production of glucoamylase by Aspergillus oryzae on the solid surface of rice husk, wheat bran, rice bran, cotton seed powder, corn steep solids, bagasse powder, coconut oil cake, and groundnut oil cake as substrates. Optimization of the SSF media and parameters resulted in a 24% increase in the glucoamylase activity. Optimum glucoamylase production (1986 μmoles of glucose produced per minute per gram of dry fermented substrate) was observed on wheat bran supplemented with 1%, (w/w) starch, 0.25%, (w/w) urea at pH 6, 100%, (v/w) initial moisture and 30°C after incubation 120 hrs. Therefore, A. oryzae can be useful in bioprocessing application for saccharification of agro-residues. Keywords: Glucoamylase, Aspergillus oryzae, solid state fermentation, agro residues DOI: 10.3126/ijls.v4i0.2892 International Journal of Life Sciences Vol.4 2010 pp.16-25

scholarly journals Lovastatin production by Aspergillus fischeri under solid state fermentation from coconut oil cake

1970 ◽  
Vol 2 (1) ◽  
pp. 26-36 ◽  
Author(s):  
Parvatham Madhu Latha ◽  
Pallem Chanakya ◽  
Manipati Srikanth
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Maximum Yield ◽  
Initial Moisture Content ◽  
Coconut Oil ◽  
Malt Extract ◽  
Fermentation Time ◽  
Aspergillus Fischeri ◽  
Fermentation Parameters ◽  
Coconut Oil Cake

The main aim of the present investigation was to optimize the fermentation parameters that enhance the maximum production of lovastatin by Aspergillus fischeri using coconut oil cake as the solid substrate under solid state fermentation. The maximum yield of lovastatin (14.77 mg/g dry substrate) using coconut oil cake as the substrate was achieved with the following optimized process parameters: fermentation time (7 days), initial moisture content (60% v/w), inoculum volume (2ml of five day old culture), initial pH (5.0), incubation temperature (30ºC), lactose (1% w/v) and malt extract (1% w/v).Keywords: Lovastatin; Aspergillus fischeri; Coconut oil cake; Fermentation parameters; OptimizationDOI: http://dx.doi.org/10.3126/njb.v2i1.5641 Nepal Journal of Biotechnology Jan.2012, Vol.2(1): 26-36 

EFFECT OF SOLID SUBSTRATE AND MOISTURE CONTENT ON PRODUCTION OF FUNGAL LIPASE IN SOLID STATE FERMENTATION

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
SHIPRA SINGH
Keyword(s):  
Enzyme Activity ◽  
Solid State ◽  
Moisture Content ◽  
Lipase Production ◽  
Solid Substrate ◽  
High Yield ◽  
Moisture Contents ◽  
Lipase Enzyme ◽  
Solid Substrates ◽  
Commercial Applications

The present study has been designed to investigate the effect of solid substrates and moisture contents on the production of extra cellular lipase enzyme. High yield of enzyme activity 281.70 U/gss was obtained with rice bran and minimum activity 162.37 U/gss was observed with coconut cake. In such case of moisture content maximum lipase production was obtained 365.00 U/gss with 5ml moistening whereas minimum was obtained at 3ml moistening solid substrate. The study will be helpful in defining moisture content and solid substrate for higher production of lipase which are helpful in industries for commercial applications

scholarly journals Optimization of Medium Composition for the Production of Neomycin by Streptomyces fradiae NCIM 2418 in Solid State Fermentation

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
B. M. Vastrad ◽  
S. E. Neelagund
Keyword(s):  
Solid State ◽  
Ammonium Nitrate ◽  
Ammonium Chloride ◽  
Solid State Fermentation ◽  
Sodium Nitrate ◽  
Coconut Oil ◽  
Medium Composition ◽  
Significant Variable ◽  
Streptomyces Fradiae ◽  
Coconut Oil Cake

Neomycin production of Streptomyces fradiae NCIM 2418 was optimized by using response surface methodology (RSM), which is powerful mathematical approach comprehensively applied in the optimization of solid state fermentation processes. In the first step of optimization, with Placket-Burman design, ammonium chloride, sodium nitrate, L-histidine, and ammonium nitrate were established to be the crucial nutritional factors affecting neomycin production significantly. In the second step, a 24 full factorial central composite design and RSM were applied to determine the optimal concentration of significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the important nutrients for the maximum were obtained as follows: ammonium chloride 2.00%, sodium nitrate 1.50%, L-histidine 0.250%, and ammonium nitrate 0.250% with a predicted value of maximum neomycin production of 20,000 g kg−1 dry coconut oil cake. Under the optimal condition, the practical neomycin production was 19,642 g kg−1 dry coconut oil cake. The determination coefficient (R2) was 0.9232, which ensures an acceptable admissibility of the model.

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