scholarly journals Effect of jasmonic acid on some biochemical and physiological parameters in salt-stressed Brassica napus seedlings

2017 ◽  
Vol 9 (4) ◽  
pp. 36-42 ◽  
Author(s):  
Kaur Harpreet ◽  
Sirhindi Geetika ◽  
Sharma Poonam
Keyword(s):  
Brassica Napus ◽  
Jasmonic Acid ◽  
Physiological Parameters

Do low-ethylene-producing transgenic canola (Brassica napus) plants expressing the ACC deaminase gene differ from wild-type plants in response to UVB radiation?

2007 ◽  
Vol 85 (2) ◽  
pp. 148-159 ◽  
Author(s):  
Mirwais M. Qaderi ◽  
M. Anisul Islam ◽  
David M. Reid ◽  
Saleh Shah
Keyword(s):  
Brassica Napus ◽  
Transgenic Plants ◽  
Photosynthetic Pigment ◽  
Physiological Parameters ◽  
Ultraviolet B ◽  
Differential Sensitivity ◽  
Plant Responses ◽  
Uvb Radiation ◽  
Wild Type ◽  
Ethylene Evolution

Few studies have considered ethylene involvement in plant responses to ultraviolet-B (UVB) radiation. We studied the responses to UVB radiation of one wild-type (WT, ‘Westar’) canola (Brassica napus L.) with normal ethylene evolution and two transgenic (C1, C2) lines with lower ethylene evolution. Canola plants were grown under biologically effective levels of UVB (UVBBE) radiation: 0.03 (low), 4.88 (medium), and 9.78 (high) kJ·m–2·d–1 in controlled-environment growth chambers. The growth and physiological parameters of the plants were measured. Of the two transgenic lines, C1 demonstrated higher ethylene evolution than C2 but lower than WT. The lowest aboveground and belowground biomass was found with exposure to high UVB radiation. WT produced more biomass than C2. Net CO2 assimilation and transpiration did not vary among plant lines or UVB treatments. Water-use efficiency was lower under high UVB radiation than under low UVB. The quantum yield of photosystem II was higher for C2 than for either WT or C1. WT did not differ from transgenic plants in respect to photosynthetic pigments and UV-screening compounds. Photosynthetic pigment concentration decreased, but concentration of UV-screening compounds, thickness of epicuticular wax, and the rate of root hydraulic conductance were increased by exposure to UVB radiation. While there appears to be a lack of ethylene involvement in some of the measured physiological parameters, the transgenic plants exhibited differential sensitivity to UVB in a few key measured parameters.

scholarly journals Transcriptome Analysis of Rlm2-Mediated Host Immunity in the Brassica napus–Leptosphaeria maculans Pathosystem

2019 ◽  
Vol 32 (8) ◽  
pp. 1001-1012 ◽  
Author(s):  
Michael G. Becker ◽  
Parham Haddadi ◽  
Joey Wan ◽  
Lorne Adam ◽  
Philip Walker ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Jasmonic Acid ◽  
Plant Resistance ◽  
Large Scale ◽  
Laser Microdissection ◽  
Leptosphaeria Maculans ◽  
Regulatory Elements ◽  
Host Immunity ◽  
Cellular Reprogramming ◽  
Physiological Evidence

Our study investigated disease resistance in the Brassica napus–Leptosphaeria maculans pathosystem using a combination of laser microdissection, dual RNA sequencing, and physiological validations of large-scale gene sets. The use of laser microdissection improved pathogen detection and identified putative L. maculans effectors and lytic enzymes operative during host colonization. Within 24 h of inoculation, we detected large shifts in gene activity in resistant cotyledons associated with jasmonic acid and calcium signaling pathways that accelerated the plant defense response. Sequencing data were validated through the direct quantification of endogenous jasmonic acid levels. Additionally, resistance against L. maculans was abolished when the calcium chelator EGTA was applied to the inoculation site, providing physiological evidence of the role of calcium in B. napus immunity against L. maculans. We integrated gene expression data with all available information on cis-regulatory elements and transcription factor binding affinities to better understand the gene regulatory networks underpinning plant resistance to hemibiotrophic pathogens. These in silico analyses point to early cellular reprogramming during host immunity that are coordinated by CAMTA, BZIP, and bHLH transcription factors. Together, we provide compelling genetic and physiological evidence into the programming of plant resistance against fungal pathogens.

p-Coumaric acid induces jasmonic acid-mediated phenolic accumulation and resistance to black rot disease in Brassica napus

2019 ◽  
Vol 106 ◽  
pp. 270-275 ◽  
Author(s):  
Md Tabibul Islam ◽  
Bok-Rye Lee ◽  
Van Hien La ◽  
Hyo Lee ◽  
Woo-Jin Jung ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Jasmonic Acid ◽  
Black Rot ◽  
Coumaric Acid ◽  
Rot Disease

scholarly journals Role of jasmonic acid in improving tolerance of rapeseed (Brassica napus L.) to Cd toxicity

2018 ◽  
Vol 19 (2) ◽  
pp. 130-146 ◽  
Author(s):  
Essa Ali ◽  
Nazim Hussain ◽  
Imran Haider Shamsi ◽  
Zahra Jabeen ◽  
Muzammil Hussain Siddiqui ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Jasmonic Acid ◽  
Brassica Napus L ◽  
Cd Toxicity

Regulation of accumulation of mRNAs encoding a 20-kDa oil-body protein in microspore-derived embryos of Brassica napus

1992 ◽  
Vol 70 (3) ◽  
pp. 503-508 ◽  
Author(s):  
Gijs J. H. van Rooijen ◽  
Ronald W. Wilen ◽  
Larry A. Holbrook ◽  
Maurice M. Moloney
Keyword(s):  
Abscisic Acid ◽  
Brassica Napus ◽  
Jasmonic Acid ◽  
Southern Blot Analysis ◽  
Time Course ◽  
Northern Analysis ◽  
Osmotic Regulation ◽  
Mrna Accumulation ◽  
Body Protein ◽  
Dna Fragment

A 990-bp DNA fragment (OB990) encoding part of a 20-kDa Brassica napus oleosin was used to investigate the expression of this gene in microspore-derived embryos. Southern blot analysis of total genomic B. napus DNA indicated that the oleosins comprise a multigene family in B. napus. Northern analysis indicated that oleosin mRNAs are seed-specific and accumulate in zygotic and microspore-derived embryos. Microspore-derived embryos were used to study the hormonal and osmotic regulation of this oleosin gene family. Oleosin mRNAs can be found as early as heart stage. The accumulation of these mRNAs increased dramatically after the application of 10 μM abscisic acid or high osmoticum (12.5% sorbitol). Time course experiments to study the induction of oleosin expression by 10 μM abscisic acid and 12.5% sorbitol demonstrated that the abscisic acid and sorbitol effects occur concurrently. This is distinct from the accumulation of napin storage protein mRNAs after the same treatments. When 10 μM jasmonic acid was applied, the oleosin mRNA accumulation increases significantly. The possibility that jasmonic acid and abscisic acid may function as intermediaries of the imposed osmotic stress is discussed. Key words: Brassica napus, gene regulation, microspore-derived embryos, osmoticum, abscisic acid, jasmonic acid.

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