Regulation of accumulation of mRNAs encoding a 20-kDa oil-body protein in microspore-derived embryos of Brassica napus

1992 ◽  
Vol 70 (3) ◽  
pp. 503-508 ◽  
Author(s):  
Gijs J. H. van Rooijen ◽  
Ronald W. Wilen ◽  
Larry A. Holbrook ◽  
Maurice M. Moloney
Keyword(s):  
Abscisic Acid ◽  
Brassica Napus ◽  
Jasmonic Acid ◽  
Southern Blot Analysis ◽  
Time Course ◽  
Northern Analysis ◽  
Osmotic Regulation ◽  
Mrna Accumulation ◽  
Body Protein ◽  
Dna Fragment

A 990-bp DNA fragment (OB990) encoding part of a 20-kDa Brassica napus oleosin was used to investigate the expression of this gene in microspore-derived embryos. Southern blot analysis of total genomic B. napus DNA indicated that the oleosins comprise a multigene family in B. napus. Northern analysis indicated that oleosin mRNAs are seed-specific and accumulate in zygotic and microspore-derived embryos. Microspore-derived embryos were used to study the hormonal and osmotic regulation of this oleosin gene family. Oleosin mRNAs can be found as early as heart stage. The accumulation of these mRNAs increased dramatically after the application of 10 μM abscisic acid or high osmoticum (12.5% sorbitol). Time course experiments to study the induction of oleosin expression by 10 μM abscisic acid and 12.5% sorbitol demonstrated that the abscisic acid and sorbitol effects occur concurrently. This is distinct from the accumulation of napin storage protein mRNAs after the same treatments. When 10 μM jasmonic acid was applied, the oleosin mRNA accumulation increases significantly. The possibility that jasmonic acid and abscisic acid may function as intermediaries of the imposed osmotic stress is discussed. Key words: Brassica napus, gene regulation, microspore-derived embryos, osmoticum, abscisic acid, jasmonic acid.

Effects of abscisic acid and temperature on erucic acid accumulation in oilseed rape (Brassica napus L.)

1997 ◽  
Vol 150 (4) ◽  
pp. 414-419 ◽  
Author(s):  
Jeroen A. Wilmer ◽  
Johannes P.F.G. Helsper ◽  
Linus H.W. van der Plas
Keyword(s):  
Abscisic Acid ◽  
Brassica Napus ◽  
Erucic Acid ◽  
Oilseed Rape ◽  
Brassica Napus L ◽  
Acid Accumulation

scholarly journals Differential Effect of Jasmonic Acid and Abscisic Acid on Cell Cycle Progression in Tobacco BY-2 Cells

2002 ◽  
Vol 128 (1) ◽  
pp. 201-211 ◽  
Author(s):  
Agnieszka Świa̧tek ◽  
Marc Lenjou ◽  
Dirk Van Bockstaele ◽  
Dirk Inzé ◽  
Harry Van Onckelen
Keyword(s):  
Cell Cycle ◽  
Abscisic Acid ◽  
Jasmonic Acid ◽  
Differential Effect ◽  
Cell Cycle Progression ◽  
Cycle Progression

Regulation of elongase activity by abscisic acid and temperature in microspore-derived embryos of oilseed rape (Brassica napus )

1998 ◽  
Vol 102 (2) ◽  
pp. 185-191 ◽  
Author(s):  
J. A. Wilmer ◽  
R. Lessire ◽  
J. P. F. G. Helsper ◽  
L. H. W. Van Der Plas
Keyword(s):  
Abscisic Acid ◽  
Brassica Napus ◽  
Oilseed Rape

Differential patterns of transcript accumulation during human myogenesis

1987 ◽  
Vol 7 (11) ◽  
pp. 4100-4114
Author(s):  
P Gunning ◽  
E Hardeman ◽  
R Wade ◽  
P Ponte ◽  
W Bains ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Carbonic Anhydrase ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Time Course ◽  
Human Skeletal Muscle ◽  
Transcript Accumulation ◽  
Mrna Accumulation ◽  
Adult Human ◽  
Alpha Actin

We evaluated the extent to which muscle-specific genes display identical patterns of mRNA accumulation during human myogenesis. Cloned satellite cells isolated from adult human skeletal muscle were expanded in culture, and RNA was isolated from low- and high-confluence cells and from fusing cultures over a 15-day time course. The accumulation of over 20 different transcripts was compared in these samples with that in fetal and adult human skeletal muscle. The expression of carbonic anhydrase 3, myoglobin, HSP83, and mRNAs encoding eight unknown proteins were examined in human myogenic cultures. In general, the expression of most of the mRNAs was induced after fusion to form myotubes. However, several exceptions, including carbonic anhydrase and myoglobin, showed no detectable expression in early myotubes. Comparison of all transcripts demonstrated little, if any, identity of mRNA accumulation patterns. Similar variability was also seen for mRNAs which were also expressed in nonmuscle cells. Accumulation of mRNAs encoding alpha-skeletal, alpha-cardiac, beta- and gamma-actin, total myosin heavy chain, and alpha- and beta-tubulin also displayed discordant regulation, which has important implications for sarcomere assembly. Cardiac actin was the only muscle-specific transcript that was detected in low-confluency cells and was the major alpha-actin mRNA at all times in fusing cultures. Skeletal actin was transiently induced in fusing cultures and then reduced by an order of magnitude. Total myosin heavy-chain mRNA accumulation lagged behind that of alpha-actin. Whereas beta- and gamma-actin displayed a sharp decrease after initiation of fusion and thereafter did not change, alpha- and beta-tubulin were transiently induced to a high level during the time course in culture. We conclude that each gene may have its own unique determinants of transcript accumulation and that the phenotype of a muscle may not be determined so much by which genes are active or silent but rather by the extent to which their transcript levels are modulated. Finally, we observed that patterns of transcript accumulation established within the myotube cultures were consistent with the hypothesis that myoblasts isolated from adult tissue recapitulate a myogenic developmental program. However, we also detected a transient appearance of adult skeletal muscle-specific transcripts in high-confluence myoblast cultures. This indicates that the initial differentiation of these myoblasts may reflect a more complex process than simple recapitulation of development.

scholarly journals Abscisic acid is essential for rewiring of jasmonic acid-dependent defenses during herbivory

2019 ◽  
Author(s):  
Irene A Vos ◽  
Adriaan Verhage ◽  
Lewis G Watt ◽  
Ido Vlaardingerbroek ◽  
Robert C Schuurink ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Jasmonic Acid ◽  
Plant Hormone ◽  
Pieris Rapae ◽  
Necrotrophic Pathogen ◽  
Plant Insect Interactions ◽  
Gcc Box ◽  
Insect Interactions ◽  
And Control

AbstractJasmonic acid (JA) is an important plant hormone in the regulation of defenses against chewing herbivores and necrotrophic pathogens. In Arabidopsis thaliana, the JA response pathway consists of two antagonistic branches that are regulated by MYC- and ERF-type transcription factors, respectively. The role of abscisic acid (ABA) and ethylene (ET) in the molecular regulation of the MYC/ERF antagonism during plant-insect interactions is still unclear. Here, we show that production of ABA induced in response to leaf-chewing Pieris rapae caterpillars is required for both the activation of the MYC-branch and the suppression of the ERF-branch during herbivory. Exogenous application of ABA suppressed ectopic ERF-mediated PDF1.2 expression in 35S::ORA59 plants. Moreover, the GCC-box promoter motif, which is required for JA/ET-induced activation of the ERF-branch genes ORA59 and PDF1.2, was targeted by ABA. Application of gaseous ET counteracted activation of the MYC-branch and repression of the ERF-branch by P. rapae, but infection with the ET-inducing necrotrophic pathogen Botrytis cinerea did not. Accordingly, P. rapae performed equally well on B. cinerea-infected and control plants, whereas activation of the MYC-branch resulted in reduced caterpillar performance. Together, these data indicate that upon feeding by P. rapae, ABA is essential for activating the MYC-branch and suppressing the ERF-branch of the JA pathway, which maximizes defense against caterpillars.

Analysis of abscisic acid responsive proteins in Brassica napus guard cells by multiplexed isobaric tagging

2010 ◽  
Vol 73 (4) ◽  
pp. 790-805 ◽  
Author(s):  
Mengmeng Zhu ◽  
Brigitte Simons ◽  
Ning Zhu ◽  
David G. Oppenheimer ◽  
Sixue Chen
Keyword(s):  
Abscisic Acid ◽  
Brassica Napus ◽  
Guard Cells ◽  
Isobaric Tagging
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