scholarly journals Neuropharmacological studies on ethyl acetate fraction of Securinega virosa root bark extract

2013 ◽  
Vol 7 (6) ◽  
pp. 275-279
Author(s):  
Mohammed Garba
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Bark Extract ◽  
Root Bark ◽  
Securinega Virosa

scholarly journals Bacteriostatic and Bactericidal effects of ethyl acetate root bark extract of Terminalia avicennioides on Methicillin-resistant Staphylococcus aureus

2018 ◽  
Vol 12 (5) ◽  
pp. 45-54
Author(s):  
Ekene Ugochukwu Adim ◽  
Emeka John Dingwoke ◽  
Fatima Amin Adamude ◽  
Chidi Edenta ◽  
Ndubuisi Nwobodo Nwobodo ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Ethyl Acetate ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Bark Extract ◽  
Root Bark ◽  
Methicillin Resistant ◽  
Bactericidal Effects

Ethyl acetate fraction of Spathodea campanulata (Bignoniaceae) attenuates lead acetate induced testicular toxicity in male Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Glory Olubusayo Adesiyan ◽  
Temitope Olaoluwa Talabi ◽  
Oluwafemi Ezekiel Kale ◽  
Temitope Funmi Ogundare
Keyword(s):  
Ethyl Acetate ◽  
Lead Acetate ◽  
Octadecenoic Acid ◽  
Morphological Characteristics ◽  
Acid Methyl Ester ◽  
Ethyl Acetate Fraction ◽  
Male Rats ◽  
Bark Extract ◽  
Seminiferous Tubules ◽  
Testicular Toxicity

Abstract Objectives The use of Spathodea campanulata in folklore medicine for the management of reproductive disorders has been poorly reported. We sought to investigate the protective potential of the ethyl acetate fraction of S. campanulata stem bark extract (EFSC) on lead acetate-induced (LA) testicular toxicity in male rats. Methods Animals during a 28 days treatment received dimethyl sulfoxide (DMSO, 0.1%), LA (20 mg/kg), and EFSC (200 mg/kg). Others received EFSC only (100, 200, and 400 mg/kg) or vitamin E (100 mg/kg) 1 h prior to LA (20 mg/kg) administration. Results LA administration decreased sperm counts and motility by 36.39 and 40.69% respectively in rats. Also, LA-untreated rats showed elevated malondialdehyde (MDA) and decreased total proteins in testis (260, 33%) and epididymis (62, 29%) respectively. However, EFSC (100, 200, or 400 mg/kg) administrations improved sperm morphological characteristics as well as antioxidant status in LA-treated rats. EFSC (400 mg/kg) showed improved testis seminiferous tubules that were almost normal in the LA-treated rats. Further, EFSC contains a high 9-octadecenoic acid methyl ester. Conclusions Overall, evidence by LA-induced testicular toxicity, EFSC provides chemopreventive roles via antioxidant mechanisms.

scholarly journals PENENTUAN POTENSI EKSTRAK KULIT BATANG TUMBUHAN SIKKAM (Bischofia javanica Blume) SEBAGAI ANTIOKSIDAN DENGAN METODE DPPH DAN SITOTOKSIK DENGAN METODE BSLT

Alotrop ◽  
2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Dapot Parulian Manurung ◽  
Agus Sundaryono ◽  
Hermansyah Amir
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Anticancer Agent ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Bark Extract ◽  
Test Results ◽  
Bischofia Javanica ◽  
Ethanol Fraction

This study aims to determine the level of antioxidant activity of Sikkam bark extract (Bischofia javanica Blume) and its potential as an anticancer agent. This research was conducted in November 2018 - April 2019 in the Laboratory of Chemical Education Guidance and Counseling and Basic Science Laboratory, University of Bengkulu. The sample in this study was obtained from Gunung Mariah village in Simalungun district, North Sumatra. The method used in this study was extraction using maceration, liquid-liquid fractionation, phytochemical test, DPPH (Diphenylpicrylhiydrazyl) test and BSLT test (Brine Shrimp Lethality Test). Extraction maceration using ethanol 96%, fractionation using a solvent n-hexane and ethyl acetate, phytochemical tests using Mayer's reagent, Lieberman-Burchard, FeCl3 1% and H2SO4 2N, antioxidant test using DPPH (1,1-diphenyl-2-picrylhydrazyl) and cytotoxic tests using shrimp Artemia salina Leach larvae. The results of the study on maceration of 800 grams of Sikkam bark obtained a crude extract of 53.6 grams (6.7%). Fractionation from 10 grams of crude extract obtained 7.24 gram ethanol fraction (72.4%), ethyl acetate fraction 2.14 gram (21.4%) and n-Hexane fraction 0.39 gram (3.9%). Phytochemical test results obtained the content of alkaloids, flavonoids, tannins, saponins and terpenoids with the highest yield on tannin compounds. The antioxidant activity test results obtained IC50 values in crude extracts of 20.94 ppm, ethanol fraction 36.29 ppm, ethyl acetate fraction 83.28 ppm and n-Hexane fraction 39.13 ppm which showed that crude extract, ethanol fraction, n-Hexane fraction were categorized as very strong antioxidants and Ethyl acetate fraction is categorized as a strong antioxidant. The cytotoxic test results using crude extract obtained an LC50 value of 54,827 ppm which showed that Sikkam bark extract was toxic so that it was potential as an anticancer agent.

scholarly journals Ethyl Acetate Fraction of Lannea microcarpa Engl. and K. Krause (Anacardiaceae) Trunk Barks Corrects Angiotensin II-Induced Hypertension and Endothelial Dysfunction in Mice

2019 ◽  
Vol 2019 ◽  
pp. 1-13
Author(s):  
Mathieu Nitiéma ◽  
Raffaella Soleti ◽  
Camille Koffi ◽  
Lazare Belemnaba ◽  
Patricia Mallegol ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Vascular Function ◽  
Ex Vivo ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Ethyl Acetate Fraction ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Ang Ii ◽  
Induced Hypertension ◽  
Cox 2

Traditional remedies prepared from Lannea microcarpa leaves, barks, roots, and fruits are used to treat many diseases including hypertension. This study investigated whether oral administration of the ethyl acetate fraction of Lannea microcarpa trunk barks (LMAE) corrects angiotensin (Ang) II-induced hypertension in mice. Its effects on vascular function were specifically investigated. Experiments explored hemodynamic and echocardiographic parameters in vivo and vascular reactivity to acetylcholine (ACh) and CaCl2ex vivo on isolated aortas. Mice received LMAE for 3 weeks (50 mg/kg/day) by oral gavage. In the last two weeks of treatment, mice were implanted with osmotic minipumps delivering NaCl (0.9%) or Ang II (0.5 mg/kg/day). LMAE completely prevented the increase in systolic and diastolic blood pressure induced by Ang II. Echocardiographic and kidney parameters were not affected by the different conditions. LMAE abrogated Ang II-induced impairment of ACh-induced relaxation without affecting that of sodium nitroprusside. LMAE also completely prevented CaCl2-induced contraction in KCl-exposed aorta ex vivo. The extract alone did not modify superoxide (O2-) and nitric oxide (NO⋅) production in femoral arteries from control mice but significantly limited Ang II-induced O2- production. These effects were associated with reduced expression of inducible isoform of cyclooxygenase- (COX-) 2 and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase isoform NOX-2 in aortas. Finally, phytochemical analysis showed that LMAE contains sterols, triterpenes, coumarins, and anthraquinone. These results showed that LMAE prevents Ang II-induced hypertension and vascular dysfunction through a reduction of oxidative stress linked to COX-2 and NOX-2 pathway and inhibition of calcium entry. This study provides pharmacological basis of the empirical use of Lannea microcarpa trunk bark extract against hypertension.

Evaluation of the antipsychotic potential of aqueous fraction of Securinega virosa root bark extract in mice

2014 ◽  
Vol 29 (1) ◽  
pp. 161-165 ◽  
Author(s):  
M. G. Magaji ◽  
M. Mohammed ◽  
R. A. Magaji ◽  
A. M. Musa ◽  
I. Abdu-Aguye ◽  
...  
Keyword(s):  
Bark Extract ◽  
Root Bark ◽  
Aqueous Fraction ◽  
Securinega Virosa

scholarly journals Antimicrobial Activity of Akanda (Calotropis gigantea L.) on Some Pathogenic Bacteria.

1970 ◽  
Vol 43 (3) ◽  
pp. 397-404 ◽  
Author(s):  
M Ashraful Alam ◽  
M Rowshanul Habib ◽  
Rarjana Nikkon ◽  
Matiar Rahman ◽  
M Rezaul Karim
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Chloroform Fraction ◽  
Root Bark ◽  
Calotropis Gigantea ◽  
Petroleum Ether Fraction ◽  
Ether Fraction

The antibacterial activity of methanol extract from the root bark of Akanda (Calotropis gigantea L.) and its petroleum ether, chloroform and ethyl acetate fractions were investigated. Both of methanol extract and its chloroform fraction showed activity against Sarcina lutea, Bacillus megaterium and Pseudomonas aeruginosa. Petroleum ether fraction showed activity against Bacillus subtilis and Shigella sonnei whereas ethyl acetate fraction showed activity against Pseudomonas aeruginosa and Escherichia coli at 20ìg/disc, 30ìg/disc and 40ìg/disc doses. Among the tested materials, methanol extract and its chloroform fraction showed comparatively better results. Minimum inhibitory concentration (MIC) for methanol extract and each fraction were also determined by serial dilution technique. Keywords: Methanol extract, Akonda (Calotropis gigantea), Petroleum ether fraction, Chloroform fraction and Ethyl acetate fraction.   DOI = 10.3329/bjsir.v43i3.1156Bangladesh J. Sci. Ind. Res. 43(3), 397-404, 2008

scholarly journals THE ETHYL ACETATE FRACTION OF PSOROSPERMUM FEBRIFUGUM SPACHROOT BARKS AQUEOUS EXTRACT IS A GOOD STIMULATOR OF HEMATOPOIESIS

2020 ◽  
Vol 8 (10) ◽  
pp. 1018-1026
Author(s):  
Maximin Senou ◽  
◽  
Pascal Atchade Tchogou ◽  
Felicienne Agbogba ◽  
Ezeckiel Jacques Lokonon ◽  
...  
Keyword(s):  
Blood Cell ◽  
Ethyl Acetate ◽  
Red Blood Cells ◽  
Aqueous Extract ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Ethyl Acetate Fraction ◽  
Root Bark ◽  
Extract Fraction ◽  
Dose Dependent

PsorospermumfebrifugumSpach. (Clusiaceae) is a medicinal plant found in Benin whose root bark was effective in treating anemia. To identify the family of chemical compounds of this organ was responsible for its hematopoietic efficiency, this work aimed to test the ethyl acetate fraction of the aqueous extract of the plant organ on anemic rats. Methods: Wistar rats were anemic by phenylhydrazinechloridrate on D0. From day 2 to day 15, some were gavage fed with the ethyl acetate fraction of PsorospermumfebrifugumSpach root bark aqueous extract at 40 or 60 mg / kg / day. Others received either vitafer as a reference drug or distilled water (untreated anemic group). Blood samples were collected from these rats and non-anemic control rats at days 0, 2, 7, 10 and 15 for the blood count and osmotic resistance of red blood cells. Results: At D2, phenylhydrazine significantly decreased hemoglobin and red blood cell number, which were corrected on D7 by the extract fraction with a dose-dependent effect. The extract fraction rapidly stimulated release of macrocytes, immature red blood cells in the first week to compensate the anemia. The extract did not affect blood platelet number, suggesting some specificity of action on the red blood cell line. Conclusion: The ethyl acetate fraction of Psorospermumfebrifugum root bark aqueous extract stimulated erythropoiesis faster than the crude extract. Its action seemed specific and dose-dependent. It would probably be related to the flavonoids which action mechanism needs to be explored.

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