scholarly journals Improvement of decontamination and isolation protocols for Mycobacterium avium subspecies paratuberculosis (MAP) from raw milk samples

2017 ◽  
Vol 11 (27) ◽  
pp. 1103-1107
Author(s):  
Azevedo Carvalho Isabel ◽  
Germano Gonçalves Schwarz David ◽  
Aparecida Grasse Pietralonga Pricila ◽  
Carolina Silva Faria Ana ◽  
Vitória Malaquias Juaci ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Raw Milk ◽  
Milk Samples

scholarly journals Mycobacterium avium Subspecies paratuberculosis DNA and Antibodies in Dairy Goat Colostrum and Milk

2019 ◽  
Vol 6 (4) ◽  
pp. 96 ◽  
Author(s):  
Karianne Lievaart-Peterson ◽  
Saskia Luttikholt ◽  
Maaike Gonggrijp ◽  
Robin Ruuls ◽  
Lars Ravesloot ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Clinical Signs ◽  
Dairy Goat ◽  
Economic Losses ◽  
Dairy Goats ◽  
Convenience Sample ◽  
Milk Samples ◽  
Zoonotic Risk ◽  
Goat Population

Mycobacterium avium subspecies paratuberculosis (MAP) is endemic in the Dutch dairy goat population causing economic loss, and negatively influencing welfare. Moreover, there are concerns about a potential zoonotic risk. Therefore the industry’s objectives are to decrease MAP prevalence, limit economic losses as well as reduce the concentration of MAP in (bulk) milk. To diminish within-farm spread of infection, vaccination, age dependent group housing with separation of newborns from adults, as well as rearing on artificial or treated colostrum and milk replacers are implemented. However, the importance of MAP contaminated colostrum and milk as a route of infection in dairy goat herds is unknown. Therefore the aim of this study was to detect the presence of MAP DNA in colostrum and milk from dairy goats in infected herds. A convenience sample of 120 colostrum samples and 202 milk samples from MAP infected dairy goat herds were tested by IS900 real-time Polymerase Chain Reaction (PCR) for MAP DNA. Furthermore, 22 colostrum samples and 27 post mortem milk samples of goats with clinical signs consistent with paratuberculosis from known infected herds were tested. The majority of samples were from goats vaccinated against MAP. Positive or doubtful PCR results were obtained in none of the 120 and two of the 22 colostrum samples, and in eight of the 202 and four of the 27 milk samples Negative PCR results were obtained in the remaining 140 (99%) colostrum samples and 217 (95%) milk samples.

scholarly journals Detection of Antibodies and Confirmation of Mycobacterium avium Subspecies paratuberculosis Using Nested PCR in Bulk Milk Samples from Nakasongola and Sembabule Districts, Uganda

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Julius Boniface Okuni ◽  
Tony Oyo ◽  
Magid Kisekka ◽  
Sylvester Ochwo ◽  
David Kalenzi Atuhaire ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Nested Pcr ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Control Program ◽  
Milk Samples ◽  
Bulk Milk ◽  
Human Inflammatory Bowel Disease ◽  
Inflammatory Bowel ◽  
Map Testing ◽  
Livestock Productivity

Mycobacterium avium subspecies paratuberculosis (MAP) is an emerging pathogen in many livestock and wildlife populations around the world. Concerns range from the serious economic impacts on livestock productivity to its suspected role in the human inflammatory bowel disease syndrome. Milk and faeces of infected animals are the main vehicles through which the organism spreads from infected to susceptible hosts. In this study, a survey was done in Nakasongola and Sembabule districts of Uganda involving a total of seven dairy collection centres to determine the prevalence of antibodies to MAP in bulk milk samples. The milk was tested with a commercial ELISA kit for MAP testing in milk. Positive and suspicious milk samples were further tested using nested PCR. Of the 257 milk samples tested, 11 (4.3%) were positive and five (1.9%) were suspicious. All the ELISA positive and suspicious milk samples were positive using nested PCR. The results show that MAP infection occurs in cattle from the two districts and highlight the need for a paratuberculosis control program in these and other districts where MAP infection has been reported.

Modeling the Occurrence of Mycobacterium avium subsp. paratuberculosis in Bulk Raw Milk and the Impact of Management Options for Exposure Mitigation

2011 ◽  
Vol 74 (7) ◽  
pp. 1126-1136 ◽  
Author(s):  
CHRISTOPHE BOULAIS ◽  
RON WACKER ◽  
JEAN-CHRISTOPHE AUGUSTIN ◽  
MOHAMED HEDI BEN CHEIKH ◽  
FABRICE PELADAN
Keyword(s):  
Mycobacterium Avium ◽  
Raw Milk ◽  
Mycobacterium Avium Subsp ◽  
Dairy Herds ◽  
Management Options ◽  
Scientific Debate ◽  
Milk Samples ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Exposure Mitigation ◽  
The Impact

Mycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of paratuberculosis (Johne's disease) in cattle and other farm ruminants. The potential role of MAP in Crohn's disease in humans and the contribution of dairy products to human exposure to MAP continue to be the subject of scientific debate. The occurrence of MAP in bulk raw milk from dairy herds was assessed using a stochastic modeling approach. Raw milk samples were collected from bulk tanks in dairy plants and tested for the presence of MAP. Results from this analytical screening were used in a Bayesian network to update the model prediction. Of the 83 raw milk samples tested, 4 were positive for MAP by culture and PCR. We estimated that the level of MAP in bulk tanks ranged from 0 CFU/ml for the 2.5th percentile to 65 CFU/ml for the 97.5th percentile, with 95% credibility intervals of [0, 0] and [16, 326], respectively. The model was used to evaluate the effect of measures aimed at reducing the occurrence of MAP in raw milk. Reducing the prevalence of paratuberculosis has less of an effect on the occurrence of MAP in bulk raw milk than does managing clinically infected animals through good farming practices.

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