scholarly journals Evaluation of biocontrol properties of Streptomyces spp. isolates against phytopathogenic fungi Colletotrichum gloeosporioides and Microcyclus ulei

2017 ◽  
Vol 11 (5) ◽  
pp. 141-154 ◽  
Author(s):  
Marcela Vinchira Villarraga Diana ◽  
Elizabeth Méndez Tibambre María ◽  
Aydee García Romero Ibonne ◽  
Rocío Suarez-Moreno Zulma ◽  
Moreno-Sarmiento Nubia
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Phytopathogenic Fungi ◽  
Streptomyces Spp ◽  
Microcyclus Ulei

scholarly journals Sorption of Zinc by exopolysaccharides produced by liquid media of phytopatogenic fungi

2021 ◽  
Vol 26 (1) ◽  
pp. 2312-2317
Author(s):  
JUAN DIEGO VALENZUELA-COBOS ◽  
ANA GRIJALVA-ENDARA
Keyword(s):  
Yeast Extract ◽  
Liquid Culture ◽  
Culture Medium ◽  
Colletotrichum Gloeosporioides ◽  
Culture Media ◽  
Phytopathogenic Fungi ◽  
Mycelial Biomass ◽  
Rhizopus Stolonifer ◽  
Liquid Media ◽  
Liquid Culture Medium

Phytopathogenic fungi such as: Colletotrichum gloeosporioides and Rhizopus stolonifer were cultivated in three different liquid culture media: LCC (glucose 40 g L-1 , yeast extract 3 g L-1 ), LC2 (glucose 40 g L-1 , yeast extract 3 g L-1 and tryptone peptone 2 g L-1 ) and LC3 (glucose 40 g L-1 , yeast extract 3 g L-1 and tryptone peptone 10 g L-1 ) under pH of 5.5 for the production of mycelial biomass and exopolysaccharides (EPS). The liquid culture medium (LC3) used in cultivation of Colletotrichum gloeosporioides showed the highest production of biomass (15.40 g L-1 ) and exopolysaccharides (3.40 g L-1 ). Exopolysaccharides (EPS) obtained from the liquid culture medium (LC3) of Colletotrichum gloeosporioides presented the highest absorption content of Zinc (56 mg g-1 ). The results presented that the exopolysaccharides (EPS) produced by Colletotrichum gloeosporioides showed the greatest biosorbent capacity of Zinc (Zn) using the culture medium with the highest amount of tryptone peptone.

scholarly journals exoenzymes as a pathogenicity factor for Colletotrichum gloeosporioides associated with coffee plants

2019 ◽  
Vol 45 (4) ◽  
pp. 368-373
Author(s):  
Cecilia Armesto ◽  
Fernanda Gonçalves Martins Maia ◽  
Fernando Pereira Monteiro ◽  
Mário Sobral de Abreu
Keyword(s):  
Cell Walls ◽  
Extracellular Enzymes ◽  
Colletotrichum Gloeosporioides ◽  
Hydrolytic Enzymes ◽  
Phytopathogenic Fungi ◽  
Specific Cell ◽  
Cell Wall Degrading Enzymes ◽  
Colonization Process ◽  
Coffee Plants ◽  
Severity Degree

ABSTRACT Phytopathogenic fungi during the penetration and colonization process are capable of secreting several enzymes, which enable infection of the host live tissue, acting on the degradation of wax, cuticle and cell walls. The ability of a pathogenic agent to produce enzymes or not can determine the severity degree of a disease. In this study, 33 isolates of Colletotrichum gloeosporioides related to anthracnose and blister spot on coffee trees were evaluated for their ability to produce hydrolytic enzymes (amylase, lipase, protease, laccase, pectinase and cellulase) and specific cell wall degrading enzymes “CWDEs” (polygalacturonase, polymethylgalacturonase and pectin-lyase), as well as their relationship with the pathogenicity/aggressiveness of isolates. For all isolates of C. gloeosporioides, extracellular enzymes could be detected, except cellulases. Isolates I-9 and I-24 produced the highest levels of extracellular enzymes, as well as CWDEs. They also had the highest disease intensity indexes, suggesting a relationship between enzymes and aggressiveness of the isolates.

scholarly journals Constituents, Antioxidant and Antifungal Properties of Jatropha dioica var. dioica

2019 ◽  
Vol 14 (5) ◽  
pp. 1934578X1985243
Author(s):  
Jorge Gutiérrez-Tlahque ◽  
César L. Aguirre-Mancilla ◽  
César López-Palestina ◽  
Rosa E. Sánchez-Fernández ◽  
Alma D. Hernández-Fuentes ◽  
...  
Keyword(s):  
Arid Regions ◽  
Colletotrichum Gloeosporioides ◽  
Radical Scavenging ◽  
Sclerotium Rolfsii ◽  
Fungal Growth ◽  
Phytopathogenic Fungi ◽  
Phytochemical Study ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Ethyl Acetate Extracts

The antioxidant and antifungal activity of n-hexane and ethyl acetate extracts of stems and roots of Jatropha dioica var. dioica, a plant species from the semi-arid regions of Hidalgo, Mexico, were evaluated by the 2,2-diphenyl-1-picrylhydrazyl and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) free-radical scavenging capacity assays and by growth inhibition of the phytopathogenic fungi, Alternaria alternata, Sclerotium rolfsii, Colletotrichum gloeosporioides, Rhizoctonia solani, and Fusarium oxysporum. The extracts exhibited a significant capacity to capture free radicals and a moderate capacity to inhibit fungal growth. A phytochemical study of these extracts led to the isolation of alkyl esters of acetic and ferulic acid, as well as the known diterpenes citlalitrione, jatrophatrione, jatropholone A, and jatropholone B, which were physically and spectroscopically characterized. These metabolites could be responsible for the observed antioxidant and fungicide activities of the extracts.

scholarly journals Integrated management of causal agents of postharvest fruit rot of apple

2011 ◽  
Vol 26 (4) ◽  
pp. 289-299 ◽  
Author(s):  
Mila Grahovac ◽  
Dusanka Indjic ◽  
Brankica Tanovic ◽  
Sanja Lazic ◽  
Slavica Vukovic ◽  
...  
Keyword(s):  
Fungal Pathogens ◽  
Colletotrichum Gloeosporioides ◽  
Integrated Management ◽  
Poor Quality ◽  
Phytopathogenic Fungi ◽  
Fruit Rot ◽  
Penicillium Expansum ◽  
Phytopathogenic Fungus ◽  
Economic Losses ◽  
Apple Fruits

One of the major causes of poor quality and fruit loss (during storage and transport) are diseases caused by phytopathogenic fungi. Economic losses which are the consequence of the phytopathogenic fungus activity after harvest exceed the losses in the field. The most important postharvest fungal pathogens of apple fruits are: Botrytis cinerea Pers. ex Fr., Penicillium expansum (Lk.) Thom., Cryptosporiopsis curvispora (Peck.) Grem., Colletotrichum gloeosporioides (Penz.) Sacc., Monilinia sp., Gloeosporium album Osterw, Alternaria alternata (Fr.) Keissler, Cladosporium herbarium Link., Cylindrocarpon mali (Alles.) Wollenw., Stemphylium botryosum Wallr. The use of available protection technologies can significantly reduce losses caused by pathogens in storage. The concept of integrated pest management (IPM) in apple fruits i.e. sustainable approach in control of causal agents of postharvest fruit rot, using cultural, physical, biological and chemical measures, to minimize economic, health and risks to consumers and environment, is presented in the paper.

Colletotrichum Gloeosporioides Species Complex: Pathogen Causing Anthracnose, Gummosis and Die-Back Diseases of Cashew (Anacardium Occidentale L.) In Ghana

2020 ◽  
Vol 2 (6) ◽  
Author(s):  
A. Muntala ◽  
P. M. Norshie ◽  
K. G. Santo ◽  
C. K. S. Saba
Keyword(s):  
Species Complex ◽  
Colletotrichum Gloeosporioides ◽  
Potato Dextrose Agar ◽  
Causal Agent ◽  
Anacardium Occidentale ◽  
Causative Organism ◽  
Pathogenicity Test ◽  
Small Water ◽  
Colletotrichum Gloeosporioides Species Complex

A survey was conducted in twenty-five cashew (Anacardium occidentale) orchards in five communities in the Dormaa-Central Municipality of Bono Region of Ghana to assess the incidence and severity of anthracnose, gummosis and die-back diseases on cashew. Cashew diseased samples of leaves, stem, inflorescences, twigs, flowers, nuts and apples showing symptoms (e. g. small, water-soaked, circular or irregular yellow, dark or brown spots or lesions on leaves, fruits and flowers, sunken surface, especially on the apples, blight, gum exudates) were collected for isolation of presumptive causative organism. The pathogen was isolated after disinfecting the excised diseased pieces in 70% ethanol, plated on potato dextrose agar (PDA) and incubated at 28 oC for 3 to 7 days. The identity of the putative pathogen was morphologically and culturally confirmed as belonging to Colletotrichum gloeosporioides species complex using standard mycological identification protocols. The pathogen had varied conidia sizes of between 9-15 up to 20 μm in length and diameter of 3-6 μm. The conidia were straight and cylindrically shaped with rounded or obtuse ends. The septate mycelium was whitish-grey, velvety and cotton-like in appearance from the top. The results confirmed the presence of the pathogen in the orchards with incidence ranging from 6.9% and 14.0% for gummosis and averaged 22.9% for anthracnose infected orchards. The result of the pathogenicity test confirmed the isolates to be pathogenic on inoculated cashew seedlings and were consistently re-isolated, thereby establishing the pathogen as the true causal agent of the said diseases in cashew trees and thus completed the Koch’s postulate.

METODOLOGIA DE INOCULAÇÃO DE Colletotrichum gloeosporioides EM FRUTOS DE MAMÃO

2012 ◽  
Vol 24 (3) ◽  
pp. 183-188
Author(s):  
Lahyre Izaete Silveira Gomes ◽  
Eduardo Alves ◽  
Enilton Nascimento de Santana ◽  
Hilário Antônio de Castro
Keyword(s):  
Colletotrichum Gloeosporioides

Preliminary Screening of Isolated Mycoherbicidal Fungi for the Management of Noxious Weed Xanthiumstrumarium

2019 ◽  
Vol 6 (02) ◽  
Author(s):  
AJAY KUMAR SINGH ◽  
AKHILESH KUMAR PANDEY
Keyword(s):  
Culture Filtrate ◽  
Photosynthetic Pigment ◽  
Phytopathogenic Fungi ◽  
Herbicidal Activity ◽  
Preliminary Screening ◽  
Detached Leaf ◽  
Noxious Weed ◽  
Complete Collapse

Natural phytotoxins of fungi are great source for the discovery of new herbicide and its offer a benign and eco-friendly alternative to manage weed. Thus, this study aimed to select potential fungi with potent herbicidal activity for control ofweeds. In the present study, various phytopathogenic fungi were isolated from infected tissues of various weeds and evaluated againstXanthium strumarium, a problematic monocotyledonous weed of open lands, agriculture, horticulture and forests. Herbicidal potential of Cell Free Culture Filtrate (CFCF) of strains ofPhoma herbarum (FGCCW#18, FGCCW#43) Fusariummonilifromecoded as FGCCW#35 and Fusarium roseum coded as FGCCW#55againstXanthium strumariumwere evaluated by seedling and shoot cut bioassays. Maximum mortalities of shoots, seedlings and phytotoxic damage were obtainedfrom28 day sold cell free culture filtrate (CFCF) of FGCCW#18 at 100% concentration. Significant reduction in biological contents i.e. photosynthetic pigment and protein was observed in the host weed on treatment with the CFCF as determined by detached leaf bioassay. Phytotoxic damage such as severe wilting, chlorosis, necrosis and complete collapse of the entire parts of the weed were also noticed due to CFCF application.

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