scholarly journals Detection of antibodies to Peste des petits ruminants virus using passive haemagglutination test and cELISA in the White Nile state-Sudan, comparative study

2014 ◽  
Vol 8 (38) ◽  
pp. 3475-3481 ◽  
Author(s):  
O. M. Ishag, ◽  
K. S. Intisar, ◽  
Y. H. Ali,
Author(s):  
Mirthes Ueda ◽  
Eide Dias Camargo ◽  
Adelaide José Vaz ◽  
Ana Maria Carvalho de Souza ◽  
Regina Maria Figueiredo ◽  
...  

A passive haemagglutination test (PHA) for human neurocysticercosis was standardized and evaluated for the detection of specific antibodies to Cysticercus cellulosae in cerebrospinal fluid (CSF). For the assay, formaldehyde-treated group O Rh-human red cells coated with the cysticerci crude total saline extract (TS) antigen were employed. A total of 115 CSF samples from patients with neurocysticercosis was analysed, of these 94 presented reactivity, corresponding to 81.7% sensitivity, in which confidence limit of 95% probability (CL95%) ranged from 74.5% to 88.9%. Eighty-nine CSF samples derived from individuals of control group presented as nonreactive in 94.4% (CL95% from 89.6% to 99.2%). The positive and negative predictive values were 1.4% and 99.9%, respectively, considering the mean rate of that this assay provide a rapid, highly reproducible, and moderately sensitive mean of detecting specific antibodies in CSF samples.


1995 ◽  
Vol 27 (1) ◽  
pp. 43-46 ◽  
Author(s):  
K. Nachimuthu ◽  
G. Dhinakar Raj ◽  
A. Thangavelu ◽  
R. A. Venkatesan

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