scholarly journals Effect of N-acyl homoserine lactones (AHLs) quorum sensing signal molecules on Enterococcus faecalis biofilm formation

2014 ◽  
Vol 8 (34) ◽  
pp. 3144-3149
Author(s):  
Bensalem Karima ◽  
Djeribi Ryad ◽  
Javier Lpez Baena Francisco
Keyword(s):  
Quorum Sensing ◽  
Enterococcus Faecalis ◽  
Biofilm Formation ◽  
Signal Molecules ◽  
Homoserine Lactones ◽  
Acyl Homoserine Lactones

Mutants of Burkholderia cenocepacia with a change in synthesis of N-acyl-homoserine lactones—Signal molecules of quorum sensing regulation

2012 ◽  
Vol 48 (5) ◽  
pp. 513-521 ◽  
Author(s):  
M. A. Veselova ◽  
V. A. Lipasova ◽  
Yu. V. Zaitseva ◽  
O. A. Koksharova ◽  
M. Yu. Chernukha ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Burkholderia Cenocepacia ◽  
Signal Molecules ◽  
Homoserine Lactones ◽  
Acyl Homoserine Lactones

scholarly journals Biofilm Formation by Marine Cobetia marina alex and Pseudoalteromonas spp: Development and Detection of Quorum Sensing N-Acyl Homoserine Lactones (AHLs) Molecules

2021 ◽  
Vol 3 (3) ◽  
Author(s):  
Samia S. Abouelkheir ◽  
Eman A. Abdelghany ◽  
Soraya A. Sabry ◽  
Hanan A. Ghozlan
Keyword(s):  
Biofilm Formation ◽  
Signal Molecules ◽  
Bacterial Strains ◽  
Factors Affecting ◽  
Homoserine Lactones ◽  
Acyl Homoserine Lactones ◽  
Single Culture ◽  
Cobetia Marina ◽  
Selection For ◽  
Antagonistic Interactions

Surfaces submerged in seawater are colonized by various microorganisms, resulting in the formation of heterogenic marine biofilms. This work aims to evaluate the biofilm formation by Cobetia marina alex and doing a comparative study between this promising strain with the two bacterial strains isolated previously from the Mediterranean seawater, Alexandria, Egypt. Three strains; Cobetia marina alex, Pseudoalteromonas sp. alex, and Pseudoalteromonas prydzensis alex were screened for biofilm formation using the crystal violet (CV) quantification method in a single culture. The values of biofilm formed were OD600= 3.0, 2.7, and 2.6, respectively leading to their selection for further evaluation. However, factors affecting biofilm formation by C. marina alex were investigated. Biofilm formation was evaluated in single and multispecies consortia. Synergistic and antagonistic interactions proved in this work lead to the belief that these bacteria have the capability to produce some interesting signal molecules N-acyl Homoserine Lactones (AHLs)

scholarly journals Use of Sinorhizobium meliloti as an Indicator for Specific Detection of Long-Chain N-Acyl Homoserine Lactones

2004 ◽  
Vol 70 (6) ◽  
pp. 3715-3723 ◽  
Author(s):  
Inmaculada Llamas ◽  
Neela Keshavan ◽  
Juan E. Gonz�lez
Keyword(s):  
Quorum Sensing ◽  
Sinorhizobium Meliloti ◽  
Paracoccus Denitrificans ◽  
Indicator Strain ◽  
Signal Molecules ◽  
Specific Detection ◽  
Homoserine Lactones ◽  
Sensing Systems ◽  
Acyl Homoserine Lactones ◽  
Long Chain

ABSTRACT Population-density-dependent gene expression in gram-negative bacteria involves the production of signal molecules characterized as N-acyl homoserine lactones (AHLs). The synthesis of AHLs by numerous microorganisms has been identified by using biosensor strains based on the Agrobacterium tumefaciens and Chromobacterium violaceum quorum-sensing systems. The symbiotic nitrogen-fixing bacterium Sinorhizobium meliloti is rapidly becoming a model organism for the study of quorum sensing. This organism harbors at least three different quorum-sensing systems (Sin, Mel, and Tra), which play a role in its symbiotic relationship with its host plant, alfalfa. The Sin system is distinguished among them for the production of long-chain AHLs, including C18-HL, the longest AHL reported so far. In this work, we show that construction of a sinI::lacZ transcriptional fusion results in a strain that detects long-chain AHLs with exquisite sensitivity. Overexpression of the SinR regulator protein from a vector promoter increases its sensitivity without loss of specificity. We also show that the resulting indicator strain can recognize long-chain AHLs produced by unrelated bacteria such as Paracoccus denitrificans and Rhodobacter capsulatus. This S. meliloti indicator strain should serve as a tool for the specific detection of long-chain AHLs in new systems.

Influence of the d/l configuration of N-acyl-homoserine lactones (AHLs) and analogues on their Lux-R dependent quorum sensing activity

2018 ◽  
Vol 77 ◽  
pp. 215-222 ◽  
Author(s):  
Si-Zhe Li ◽  
Rui Xu ◽  
Mohammed Ahmar ◽  
Catherine Goux-Henry ◽  
Yves Queneau ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Homoserine Lactones ◽  
Acyl Homoserine Lactones
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