Isolation and characterization of a denitrifying Acinetobacter baumannii H1 using NO2--N as nitrogen source from shrimp farming ponds

2012 ◽  
Vol 6 (10) ◽  
Author(s):  
Haipeng Cao
Keyword(s):  
Acinetobacter Baumannii ◽  
Nitrogen Source ◽  
Shrimp Farming ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of Anoxygenic phototrophs from shrimp ponds in Thailand

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Xelimar Ramirez ◽  
Imeleta Luamanu ◽  
Ruben Michael Ceballos ◽  
Elizabeth Padilla Crespo
Keyword(s):  
Environmental Samples ◽  
Production Systems ◽  
Phenotypic Diversity ◽  
Purple Bacteria ◽  
Shrimp Farming ◽  
Post Inoculation ◽  
Shrimp Ponds ◽  
Anoxygenic Phototrophs ◽  
Isolation And Characterization

Anoxygenic phototrophic purple bacteria are ubiquitous in aquatic and terrestrial environments and demonstrate broad phenotypic diversity. Purple bacteriaderive energy from light under anaerobic conditions via anoxygenic photosynthesis, a process in which water is not the electron donor. It has been suggested that these bacteria are useful for a variety of applications, including: wastewater treatment; heavy metal remediation; nitrogen fixation; and, control of CH4 emissions. In this study, the goal was to isolate and characterize PNSB from shrimp ponds in Thailand. Surface water and sediment were collected. Enrichment cultures were prepared using Pfenning’s mineral media. As indicated by development of reddish color and turbidity, anoxygenic phototrophic growth was observed within two days of incubation. Cultures in liquid media and on solid plates exhibited a deep red or purple color ten weeks post-inoculation. Under light microscopy, enrichments consist of communities dominated by thin, elongated gram-negative cells with granules of elemental sulfur, which are characteristic of purple bacteria. Molecular methods confirm the presence of pufLM, a genetic biomarker for purple bacteria (e.g., Thiohalocapsa marina, Allochromatium vinosum, Roseovarius tolerans). Initial sequencing of key genes (i.e., pufLM) indicate that these environmental samples contain novel isolates or “geographic variants” that have not been previously described. We have developed a few pure cultures of multiple species from these environmental samples. Since shrimp farming is a key industry in southern Thailand, the characterization of the microbial communities in these ecosystems, including anoxygenic phototrophs, will provide insights into how to maintain water quality in these food production systems.

scholarly journals Isolation and characterization of potential probiotic bacteria from pustulose ark (Anadara tuberculosa) suitable for shrimp farming

2015 ◽  
Vol 43 (1) ◽  
pp. 123-136 ◽  
Author(s):  
Ana Claudia Sanchez Ortiz ◽  
Antonio Luna Gonzalez ◽  
Angel Isidro Campa Cordova ◽  
Ruth Escamilla Montes ◽  
Maria del Carmen Flores Miranda ◽  
...  
Keyword(s):  
Shrimp Farming ◽  
Probiotic Bacteria ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of an Autoinducer Synthase from Acinetobacter baumannii

2008 ◽  
Vol 190 (9) ◽  
pp. 3386-3392 ◽  
Author(s):  
Chen Niu ◽  
Katy M. Clemmer ◽  
Robert A. Bonomo ◽  
Philip N. Rather
Keyword(s):  
Acinetobacter Baumannii ◽  
Homoserine Lactone ◽  
Transcriptional Level ◽  
Human Pathogen ◽  
Isolation And Characterization ◽  
Opportunistic Human Pathogen ◽  
Biofilm Development ◽  
Culture Supernatants ◽  
Ethyl Acetate Extracts

ABSTRACT The opportunistic human pathogen Acinetobacter baumannii strain M2 was found to produce distinct acyl-homoserine lactone (AHL) signals based on the use of an Agrobacterium tumefaciens traG-lacZ biosensor. An A. baumannii gene, designated abaI, was cloned and directed AHL production in recombinant Escherichia coli. The AbaI protein was similar to members of the LuxI family of autoinducer synthases and was predicted to be the only autoinducer synthase encoded by A. baumannii. The primary AHL signal directed by AbaI was identified by mass spectrometry as being N-(3-hydroxydodecanoyl)-l-HSL (3-hydroxy-C12-HSL). Minor amounts of at least five additional AHLs were also identified. The expression of abaI at the transcriptional level was activated by ethyl acetate extracts of culture supernatants or by synthetic 3-hydroxy-C12-HSL. An abaI::Km mutant failed to produce any detectable AHL signals and was impaired in biofilm development.

scholarly journals Isolation and Characterization of Acinetobacter baumannii Recovered from Campylobacter Selective Medium

2016 ◽  
Vol 7 ◽  
Author(s):  
Dinesh M. Fernando ◽  
Izhar U. H. Khan ◽  
Rakesh Patidar ◽  
David R. Lapen ◽  
Guylaine Talbot ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Selective Medium ◽  
Isolation And Characterization

Isolation and characterization of ϕAB2: a novel bacteriophage of Acinetobacter baumannii

2010 ◽  
Vol 161 (4) ◽  
pp. 308-314 ◽  
Author(s):  
Nien-Tsung Lin ◽  
Pei-Yu Chiou ◽  
Kai-Chih Chang ◽  
Li-Kuang Chen ◽  
Meng-Jiun Lai
Keyword(s):  
Acinetobacter Baumannii ◽  
Isolation And Characterization
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