scholarly journals Isolation and Characterization of Acinetobacter baumannii Recovered from Campylobacter Selective Medium

2016 ◽  
Vol 7 ◽  
Author(s):  
Dinesh M. Fernando ◽  
Izhar U. H. Khan ◽  
Rakesh Patidar ◽  
David R. Lapen ◽  
Guylaine Talbot ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Selective Medium ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of an Autoinducer Synthase from Acinetobacter baumannii

2008 ◽  
Vol 190 (9) ◽  
pp. 3386-3392 ◽  
Author(s):  
Chen Niu ◽  
Katy M. Clemmer ◽  
Robert A. Bonomo ◽  
Philip N. Rather
Keyword(s):  
Acinetobacter Baumannii ◽  
Homoserine Lactone ◽  
Transcriptional Level ◽  
Human Pathogen ◽  
Isolation And Characterization ◽  
Opportunistic Human Pathogen ◽  
Biofilm Development ◽  
Culture Supernatants ◽  
Ethyl Acetate Extracts

ABSTRACT The opportunistic human pathogen Acinetobacter baumannii strain M2 was found to produce distinct acyl-homoserine lactone (AHL) signals based on the use of an Agrobacterium tumefaciens traG-lacZ biosensor. An A. baumannii gene, designated abaI, was cloned and directed AHL production in recombinant Escherichia coli. The AbaI protein was similar to members of the LuxI family of autoinducer synthases and was predicted to be the only autoinducer synthase encoded by A. baumannii. The primary AHL signal directed by AbaI was identified by mass spectrometry as being N-(3-hydroxydodecanoyl)-l-HSL (3-hydroxy-C12-HSL). Minor amounts of at least five additional AHLs were also identified. The expression of abaI at the transcriptional level was activated by ethyl acetate extracts of culture supernatants or by synthetic 3-hydroxy-C12-HSL. An abaI::Km mutant failed to produce any detectable AHL signals and was impaired in biofilm development.

Isolation and characterization of ϕAB2: a novel bacteriophage of Acinetobacter baumannii

2010 ◽  
Vol 161 (4) ◽  
pp. 308-314 ◽  
Author(s):  
Nien-Tsung Lin ◽  
Pei-Yu Chiou ◽  
Kai-Chih Chang ◽  
Li-Kuang Chen ◽  
Meng-Jiun Lai
Keyword(s):  
Acinetobacter Baumannii ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of Schizosaccharomyces pombe mutants affected in mitotic recombination.

Genetics ◽  
1991 ◽  
Vol 128 (3) ◽  
pp. 495-504
Author(s):  
A Gysler-Junker ◽  
Z Bodi ◽  
J Kohli
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Mitotic Recombination ◽  
Selective Medium ◽  
Intragenic Recombination ◽  
Crossing Over ◽  
Wild Type ◽  
Isolation And Characterization ◽  
Mitotic Gene Conversion ◽  
Type Frequency

Abstract A haploid Schizosaccharomyces pombe strain carrying a heteroallelic duplication of the ade6 gene was used to isolate mitotic recombination-deficient mutants. Recombination between the different copies of the ade6 gene can lead to Ade+ segregants. These are observed as growing papillae when colonies of a suitable size are replicated onto selective medium. We isolated mutants which show an altered papillation phenotype. With two exceptions, they exhibit a decrease in the frequency of mitotic recombination between the heteroalleles of the duplication. The two other mutants display a hyper-recombination phenotype. The 12 mutations were allocated to at least nine distinct loci by recombination tests. Of the eight rec mutants analyzed further, six were also affected in mitotic intergenic recombination in the intervals cen2-mat or cen3-arg 1. No effect on mitotic intragenic recombination was observed. These data suggest that mitotic gene conversion and crossing over can be separated mutationally. Meiotic recombination occurs at the wild-type frequency in all mutants investigated.

scholarly journals Glycosphingolipids Recognized by Acinetobacter baumannii

2020 ◽  
Vol 8 (4) ◽  
pp. 612
Author(s):  
Miralda Madar Johansson ◽  
Mehjar Azzouz ◽  
Beatrice Häggendal ◽  
Karin Säljö ◽  
Henri Malmi ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Bacterial Pathogen ◽  
Basic Element ◽  
Target Tissue ◽  
Binding Assays ◽  
Isolation And Characterization ◽  
Rabbit Small Intestine ◽  
Host Cell Surface ◽  
Hospital Acquired

Acinetobacter baumannii is an opportunistic bacterial pathogen associated with hospital-acquired infections, including pneumonia, meningitis, bacteremia, urinary tract infection, and wound infections. Recognition of host cell surface carbohydrates plays a crucial role in adhesion and enables microbes to colonize different host niches. Here the potential glycosphingolipid receptors of A. baumannii were examined by binding of 35S-labeled bacteria to glycosphingolipids on thin-layer chromatograms. Thereby a selective interaction with two non-acid glycosphingolipids of human and rabbit small intestine was found. The binding-active glycosphingolipids were isolated and, on the basis of mass spectrometry, identified as neolactotetraosylceramide (Galβ4GlcNAcβ3Galβ4Glcβ1Cer) and lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer). Further binding assays using reference glycosphingolipids showed that A. baumannii also bound to lactotriaosylceramide (GlcNAcβ3Galβ4Glcβ1Cer) demonstrating that GlcNAc was the basic element recognized. In addition, the bacteria occasionally bound to galactosylceramide, lactosylceramide with phytosphingosine and/or hydroxy fatty acids, isoglobotriaosylceramide, gangliotriaosylceramide, and gangliotetraosylceramide, in analogy with binding patterns that previously have been described for other bacteria classified as “lactosylceramide-binding”. Finally, by isolation and characterization of glycosphingolipids from human skin, the presence of neolactotetraosylceramide was demonstrated in this A. baumannii target tissue.

scholarly journals Isolation and Characterization of Acinetobacter baumannii from Chicken Meat Samples in North India

2021 ◽  
Vol 10 (2) ◽  
pp. 462-468
Author(s):  
Sunil Kumar ◽  
Razique Anwer ◽  
Mukesh Yadav ◽  
Nirmala Sehrawat ◽  
Vikas Kumar ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Chicken Meat ◽  
North India ◽  
Isolation And Characterization ◽  
Meat Samples

scholarly journals ISOLATION AND CHARACTERIZATION OF SULFUR-OXIDIZING BACTERIA (SOB) FROM WASTE OF RUBBER FACTORY AND PAPER IN JAMBI

2019 ◽  
Vol 1 (2) ◽  
pp. 131-139
Author(s):  
Suparjo
Keyword(s):  
Soil Fertility ◽  
Biochemical Characterization ◽  
Selective Medium ◽  
High Concentration ◽  
Isolation And Characterization ◽  
Sulfur Bacteria ◽  
Ph Reduction ◽  
Sulfur Oxidizing ◽  
Different Sources

Sewage contains high concentration of sulfate and contaminates ground water supply. The production process in the factory produced sewage containing high amount of sulfur such as sulphide and sulphate. Sulfur bacteria, which are capable of oxidizing, reducing or partially oxidizing inorganic sulfur compounds such as Thiomicrospira, Thiothrix, Desulforomonas, Achromatium, and a group of Thiobacillus. Utilization of sulfur bacteria has done in attemp to find sewage bioremediation agents as well as to promote soil fertility. Isolation and characterization were carried out by growing the bacteria in the starkey selective medium, including pH reduction test, morphology, physiology and biochemical characterization. It has been observed that out of 2 isolates from different sources of samples (rubber and paper sewage), only one isolates related to sulfur-oxidizing bacterium (SOB) while  the isolates AE01 related to true sulfur-oxidizing bacterium in genus Thiobacillus.

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