scholarly journals Somatic embryogenesis from zygotic embryos and thin cell layers (TCLs) of Brazilian oil palm (Elaeis guineensis Elaeis oleifera)

2016 ◽  
Vol 15 (37) ◽  
pp. 2028-2037 ◽  
Author(s):  
K. A. P. Bonetti ◽  
J. Nesi ◽  
R. C. Quisen ◽  
M. Quoirin
Keyword(s):  
Somatic Embryogenesis ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Zygotic Embryos ◽  
Elaeis Oleifera ◽  
Thin Cell Layers ◽  
Cell Layers

scholarly journals Effect of culture media, auxins and genotypes on plantlet regeneration from oil palm (Elaeis guineensis Jacq.) zygotic embryos through somatic embryogenesis

2021 ◽  
Vol 42 (5) ◽  
pp. 1232-1238
Author(s):  
D.S. Sparjanbabu ◽  
◽  
P.N. Kumar ◽  
S.R.K. Motukuri ◽  
D. Ramajayam ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Oil Palm ◽  
Callus Induction ◽  
Elaeis Guineensis ◽  
Culture Media ◽  
Plantlet Regeneration ◽  
Zygotic Embryos ◽  
Embryogenic Calli ◽  
Significant Difference

Aim: This study evaluated efficient culture media for the regeneration of elite material through somatic embryogenesis from oil palm zygotic embryos. Methodology: For callus induction, zygotic embryos of four elite genotypes (G1-264T, G2-238DX17P, G3-37DX17P and G4-237T) were cultured on three basal media (Y3, MS and N6) with different auxin 2 mg l-1 (Picloram, 2,4-D and Dicamba) combinations. Subculture was made every month for three passages. It evaluated various callus characters. The embryogenic calli from callus induction media were transferred to the embryo maturation medium and subcultured until the polyembryoids formed. For shoot and root formation, somatic embryo clumps were transferred into regeneration media. In-vitro plantlets with well-grown roots were hardened in pots for six weeks and assessed clonal fidelity using polymorphic SSR primers. Results: Among the treatments, calli from N6+2,4-D, Y3+2,4-D and N6+Picloram showed the highest embryogenic callus potential. G4-237T induced more embryogenic calli (32.982) among genotypes, which was on par with G1-264T (24.196). Embryogenic calli grown on N6 media with Dicamba showed the highest proliferation rate (1.141). After 60 days of culture on regeneration media, the highest number of plantlets per somatic embryogenic clump was obtained from G1-264T on N6 media supplemented with Dicamba. Interpretation: Culture media salt concentration showed a significant difference among media by causing perturbations of auxin flow during somatic embryogenesis affecting callus induction, proliferation and plantlet regeneration. This may be useful for standardizing the genotype-specific regeneration media in oil palm.

Somatic embryogenesis and plant regeneration from pistil thin cell layers of Citrus

1999 ◽  
Vol 18 (11) ◽  
pp. 935-940 ◽  
Author(s):  
F. Carimi ◽  
F. De Pasquale ◽  
F. G. Crescimanno
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Thin Cell Layers ◽  
Cell Layers

scholarly journals Physicochemical characterization of bunches from American oil palm (Elaeis oleifera H.B.K. Cortes) and their hybrids with African oil palm (Elaeis guineensis Jacq.)

2018 ◽  
Vol 67 (1) ◽  
pp. 170-178 ◽  
Author(s):  
Gabriel Chaves ◽  
Gustavo Adolfo Ligarreto- Moreno ◽  
Daniel Gerardo Cayon-Salinas
Keyword(s):  
Oil Palm ◽  
Elaeis Guineensis ◽  
Physicochemical Characterization ◽  
Elaeis Oleifera ◽  
Elaeis Guineensis Jacq ◽  
African Oil Palm

El objetivo de este estudio fue realizar un análisis comparativo de las características físicas y químicas de racimos de genotipos de Elaeis oleifera y de sus híbridos interespecíficos OxG con Elaeis guineensis, determinando los componentes y el potencial del aceite del racimo, y la calidad de los aceites, analizando el contenido de ácidos grasos, vitamina E y carotenos. En el estudio se utilizaron racimos provenientes de inflorescencias sin polinización asistida con la presencia perimetral de E. guineensis. Se utilizó un diseño experimental completamente al azar con tres unidades experimentales, cada una conformada por tres racimos. Los mayores cuajados del fruto se encontraron en el genotipo de E. oleifera Sinú (76,53 %) y el híbrido OxG II (72,64 %). Los potenciales de extracción de aceite fueron superiores en los materiales híbridos OxG destacándose el II (20,82 %). Las palmas E. oleifera presentaron mejores perfiles de ácidos grasos, destacándose los materiales del genotipo Sinú (79,1 % de ácidos grasos insaturados) y los del híbrido II (70,2 %). Para el contenido de vitamina E se confirmó la alta calidad del aceite de los materiales de E. oleifera, sobresaliendo el genotipo Coarí (1.006,7 ppm) y el híbrido II (1.549,6 ppm); el material del genotipo Sinú registró el mayor contenido de carotenos totales (1.524,7 ppm).

scholarly journals In vitro ROOTING OF TENERA HYBRID OIL PALM (Elaeis guineensis Jacq.) PLANTS1

2018 ◽  
Vol 41 (4) ◽  
Author(s):  
Marlúcia Souza Pádua ◽  
Raíssa Silveira Santos ◽  
Luciano Vilela Paiva ◽  
Vanessa Cristina Stein ◽  
Luciano Coutinho Silva
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Oil Palm ◽  
Somatic Embryos ◽  
Large Scale ◽  
Elaeis Guineensis ◽  
Germination Percentage ◽  
Shoot Length ◽  
Indole Butyric Acid

ABSTRACT Oil palm is a woody monocot of economic importance due to high oil production from its fruits. Currently, the conventional method most used to propagate oil palm is seed germination, but success is limited by long time requirements and low germination percentage. An alternative for large-scale propagation of oil palm is the biotechnological technique of somatic embryogenesis. The rooting of plants germinated from somatic embryos is a difficult step, yet it is of great importance for later acclimatization and success in propagation. The aim of this study was to evaluate the effect of the auxins indole acetic acid (IAA) and indole butyric acid (IBA) on the rooting of somatic embryos of Tenera hybrid oil palm. Plants obtained by somatic embryogenesis were inoculated in modified MS medium with 10% sucrose and 0.6% agar and supplemented with IAA or IBA at concentrations of 5 µM, 10 µM, and 15 µM, and the absence of growth regulators. After 120 days, the presence of roots, root type, length of the longest root, number of roots, number of leaves, and shoot length were analyzed. Growth regulators were favorable to rooting; plants cultivated with IBA growth regulator at 15 µM showed higher rooting percentage (87%) and better results for the parameters of number of roots (1.33) and shoot length (9.83).

scholarly journals Impacts of the dry season on the gas exchange of oil palm (Elaeis guineensis) and interspecific hybrid (Elaeis oleífera x Elaeis guineensis) progenies under field conditions in eastern Colombia

2016 ◽  
Vol 34 (3) ◽  
pp. 329-335 ◽  
Author(s):  
Cristihian Jarri Bayona-Rodríguez ◽  
Iván Ochoa-Cadavid ◽  
Hernán Mauricio Romero
Keyword(s):  
Water Stress ◽  
Gas Exchange ◽  
Water Potential ◽  
Oil Palm ◽  
Interspecific Hybrid ◽  
Elaeis Guineensis ◽  
Dry Season ◽  
Wet Season ◽  
Elaeis Oleifera ◽  
Photosyn Thesis

Elaeis guineensis palms and its interspecific hybrid (E. oleifera x E. guineensis) were planted in 2004 in the Cuernavaca farm of Unipalma S.A., located in the municipality of Paratebueno (Cundinamarca, Colombia). The palms were planted in two fields: Mecasaragua and Aurora. The first field has never been irrigated, and the second one (Aurora) has always been flood-irrigated during the dry season according to the parameters of the plantation. In this study, physiological parameters (gas exchange and water potential) were assessed in three seasons of the year 2013 (dry season, dry-to-wet transition season and wet season). Significant gas exchange differences were found among the seasons in the field with no irrigation (Mecasaragua). Likewise, differences between the genetic materials were observed during the dry season. For example, the photosyn thesis decreased by 75% compared with the palms planted in the irrigated field. No differences among seasons or materials were found in the irrigated field (Aurora). E. guineensis palms were more sensitive to water stress compared with the OxG interspecific hybrid. Both genetic materials responded rapidly to the first rains by leveling their photosynthetic rates and demonstrated an excellent capacity to recover from water stress.

scholarly journals Phenology of the oil palm interspecific hybrid Elaeis oleifera × Elaeis guineensis

2012 ◽  
Vol 69 (4) ◽  
pp. 275-280 ◽  
Author(s):  
Paola Hormaza ◽  
Eloina Mesa Fuquen ◽  
Hernán Mauricio Romero
Keyword(s):  
Oil Palm ◽  
Interspecific Hybrid ◽  
Elaeis Guineensis ◽  
Elaeis Oleifera

OIL PALM BUD ROT IN LATIN AMERICA

2003 ◽  
Vol 39 (3) ◽  
pp. 225-240 ◽  
Author(s):  
H. DE FRANQUEVILLE
Keyword(s):  
Latin America ◽  
Oil Palm ◽  
Native Species ◽  
Large Scale ◽  
Elaeis Guineensis ◽  
Physiological Disorder ◽  
Elaeis Oleifera ◽  
Infectious Origin ◽  
Holding Back

In South and Central America, tens of thousands of hectares of oil palms (Elaeis guineensis Jacq.) are affected by bud-rot types of disease. Having destroyed entire estates in Panama, Colombia, Suriname, Brazil and Ecuador, they are holding back the development of oil palm cultivation in Latin America. The cause is unknown. Indeed, 30 years after these diseases first wreaked havoc on a large scale, it is still not known whether we are dealing with one or more diseases of infectious origin, or with a physiological disorder. Despite lengthy research launched in the early 1980s, no pathogens or insect vectors have been clearly identified. At present, genetics look likely to offer a solution in the medium- to long term, using traits of resistance transmitted by the native species on the American continent, Elaeis oleifera, to the interspecific hybrid E. oleifera×E. guineensis.

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