Somatic embryogenesis and plant regeneration from pistil thin cell layers of Citrus

1999 ◽  
Vol 18 (11) ◽  
pp. 935-940 ◽  
Author(s):  
F. Carimi ◽  
F. De Pasquale ◽  
F. G. Crescimanno
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Thin Cell Layers ◽  
Cell Layers

scholarly journals Primary and secondary somatic embryogenesis in Jatropha curcas L. From leaf transverse thin cell layers

2018 ◽  
Vol 14 (4) ◽  
pp. 661-671
Author(s):  
Nguyen Thi Kim Loan ◽  
Do Dang Giap ◽  
Tran Trong Tuan ◽  
Nguyen Thi Thanh Hien ◽  
Nguyen Phuc Huy ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Jatropha Curcas ◽  
Somatic Embryos ◽  
Formation Rate ◽  
Ms Medium ◽  
Secondary Somatic Embryogenesis ◽  
Thin Cell Layers ◽  
Cell Layers ◽  
Secondary Embryos ◽  
Transverse Thin Cell Layers

An efficient method for plant regeneration in Jatropha curcas L. via primary and secondary somatic embryogenesis culture from ex vitro leaves of 6-month-old plants was presented in this study. Leaves were cut into transverse thin cell layers (tTCLs) and cultured on MS medium supplemented with kinetin (KIN) at 0.5, 1.0, 1.5, and 2.0 mg/l in combination with indole-3-butyric acid (IBA) at 0.1, 0.5, and 1.0 mg/l or 2,4-dichlorophenoxyacetic acid (2,4-D) at 1.0, 1.5 and 2.0 mg/l . The highest embryogenic callus formation rate (89.3%) was obtained on medium supplemented with 1.0 mg/l KIN and 1.5 mg/l 2,4-D. The calli were selected for the study of primary somatic embryogenesis on MS medium containing 2,4-D (0.01, 0.03, 0.05, and 0.07 mg/l) or KIN (0.5, 1.0, 1.5, and 2.0 mg/l). The highest primary somatic embryos formation rate (76.67%) was achieved on MS medium supplemented with 1.0 mg/l KIN. The primary embryos were cultured on medium supplemented with KIN (0.1, 0.5, 1.0, 1.5, and 2.0 mg/l) combined with 0.2 mg/l indole-3-butyric acid (IBA) or 0.05 mg/l 2,4-D. The combination of 1.5 mg/l KIN and 0.05 mg/l 2,4-D was suitable for secondary embryos formation. Embryos proliferated rapidly, and the highest number of secondary embryos (77.5 embryos) wasobtained from a single primary embryos inoculated. Results also showed that the addition of proline (0.75 g/l) or spermidine (0.15 mM) to the culture medium increased the number of secondary embryos considerably. The fully developed plantlets exhibiting healthy roots and shoots were obtained when somatic embryos were sub-cultured onto B5 medium containing 1.5 mg/l IBA.

scholarly journals High frequency plant regeneration from transverse thin cell layers in Indian mustard (Brassica juncea L.)

2009 ◽  
Vol 36 (1) ◽  
pp. 81-86 ◽  
Author(s):  
Mohammed Shafi Ullah Bhuiyan ◽  
Yong-Pyo Lim ◽  
Sung-Ran Min ◽  
Kwan-Sam Choi ◽  
Jang-R. Liu
Keyword(s):  
Plant Regeneration ◽  
Brassica Juncea ◽  
High Frequency ◽  
Indian Mustard ◽  
Thin Cell Layers ◽  
Cell Layers ◽  
Transverse Thin Cell Layers

scholarly journals Somatic embryogenesis from stem thin cell layers of Dendrobium aqueum

2018 ◽  
Vol 62 (3) ◽  
pp. 439-450 ◽  
Author(s):  
S. Parthibhan ◽  
M. Rao ◽  
J. A. Teixeira da Silva ◽  
T. Kumar
Keyword(s):  
Somatic Embryogenesis ◽  
Thin Cell Layers ◽  
Cell Layers
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