scholarly journals Biochemical profile of cuttings used for in vitro organogenesis of Prunus africana: An endangered species in Cameroon

2015 ◽  
Vol 14 (33) ◽  
pp. 2568-2575
Author(s):  
Germo Nzweundji Justine ◽  
Niemenak Nicolas ◽  
Oumar ◽  
Judith Tsafack Julie ◽  
Konan Koffi ◽  
...  
Keyword(s):  
Endangered Species ◽  
Biochemical Profile ◽  
In Vitro Organogenesis ◽  
Prunus Africana

scholarly journals An efficient protocol for in vitro organogenesis and antioxidant studies in Melia dubia Cav.

2016 ◽  
Vol 15 (19) ◽  
pp. 768-775 ◽  
Author(s):  
Naseer Shah Syed ◽  
Ahmad Wani Tareq ◽  
Ram Bhimi ◽  
Koul Monika ◽  
Awasthi Praveen ◽  
...  
Keyword(s):  
In Vitro Organogenesis ◽  
Melia Dubia

In vitro organogenesis and plant regeneration from unpollinated ovary cultures of Azadirachta indica

2009 ◽  
Vol 53 (2) ◽  
pp. 360-364 ◽  
Author(s):  
P. Srivastava ◽  
M. Singh ◽  
P. Mathur ◽  
R. Chaturvedi
Keyword(s):  
Plant Regeneration ◽  
Azadirachta Indica ◽  
In Vitro Organogenesis

scholarly journals In Vitro Assessment of Efficacy and Cytotoxicity of Prunus africana Extracts on Prostate Cancer C4-2 Cells

2021 ◽  
Author(s):  
Peace C. Asuzu ◽  
Alberta N.A. Aryee ◽  
Nicholas Trompeter ◽  
Yasmin Mann ◽  
Samuel A. Besong ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Lncap Cells ◽  
Leaf Extracts ◽  
Cytotoxic Effects ◽  
Prunus Africana ◽  
Plant Parts ◽  
Dependent Inhibition ◽  
Hormone Sensitive ◽  
In Vitro Assessment

AbstractPhenolic compounds are products of secondary plant metabolism known for their biological activity including their antimicrobial, antioxidant, analgesic, stimulant, anti- carcinogenic, and aphrodisiac properties. The main objective of this study was to assess the potency/cytotoxic effects of Prunus africana extracts on prostate cancer cells in vitro. Using different concentrations of P. africana extracts, prostate cancer C4-2 cells, a hormonally insensitive subline of LNCaP cells, were treated in a proliferation assay. A concentration dependent inhibition of cell growth in cells treated with P. africana bark and root extracts was present from days 1 through 3 of incubation, with the methanol extract of the bark showing the strongest effect. Compared to other plant parts, leaf extracts were significantly less cytotoxic at the same concentrations. As C4-2 cells are hormonally insensitive and designed to mimic advanced prostate cancer, crude extracts of P. africana are a possible treatment option, not only for hormone sensitive prostate cancer, but also advanced, hormonally insensitive prostate cancer.

scholarly journals Genetics of in vitro organogenesis and precocious germination of wheat embryos

1998 ◽  
Vol 21 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Claudia E. Lange ◽  
Luiz C. Federizzi ◽  
Fernando I.F. Carvalho ◽  
Ana L.C. Dornelles ◽  
Cristine L. Handel
Keyword(s):  
Gene Action ◽  
Precocious Germination ◽  
Genetic Determination ◽  
Wheat Embryo ◽  
In Vitro Organogenesis ◽  
Generation Means ◽  
Generation Means Analysis ◽  
Wheat Embryos ◽  
Segregating Population

The genetic bases of in vitro organogenesis and precocious germination of embryos in immature wheat embryo culture were investigated using six Brazilian genotypes and their F1, F2, BC1F1 and BC2F1 generations in a generation means analysis. Four parents and one set of F1’s were also analyzed in a diallel experiment. The results indicated a complex gene action controlling both traits, with additive, dominant and epistatic effects. High broad sense heritability values were found, indicating genetic determination. Considering the complexity of gene control, genetic gain could be achieved by selecting for the traits in advanced generations of the segregating population. Low correlation values between organogenesis, precocious germination, regeneration and somatic embryogenesis (data shown in a previous report) indicated the possibility of obtaining recombinant genotypes.

scholarly journals Pengaruh BAP Dan NAA Terhadap Waktu Pertumbuhan Tanaman Kantong Semar(Nepenthes adrianii) Secara In-Vitro

2018 ◽  
Author(s):  
Egi Nuryadin
Keyword(s):  
International Trade ◽  
Endangered Species

Nepenthes adrianii (Kantong Semar) merupakan tanaman endemik khas Gunung Slamet, termasuk da-lam Convention on International Trade of Endangered Species(CITES).Salahsatu potensi Nepenthes adrianii sebagai pengendali se-rangga dan tanaman obat. Penelitian ini bertujuan untuk: 1)menentukan konsentrasi interaksi BAP dan NAA yang paling baik dan waktu munculnya untuktunas, akar dan daun. Metode yang digunakan adalah metode eksperimental.Tahap munculnya tunas, akar dan daunmenggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan 2 faktor. Faktor I adalah kon-sentrasi BAP yang terdiri dari 4taraf yaitu0μM, 5 μM, 10 μM, dan 15 μM, Faktor II konsentrasi NAAyang terdiri dari 4 taraf yaitu yaitu 0μM, 0,5 μM, 1 μM, dan 1,5 μM. Hasil penelitian menunjukan bahwa perkecambahan biji tanaman Nepenthes adrianii dengan menggunakan media Vacin and Went (VW) dengan penambahan konsentrasi BAP 4 Mdan perlakuan interaksi BAP 10 M dan NAA 0,5Madalah waktu yang paling cepat dalam muncul tunas, akar dan daun.

scholarly journals In Vitro Propagation of an Endangered Helianthus verticillatus by Axillary Bud Proliferation

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 712
Author(s):  
Marzena Nowakowska ◽  
Žaklina Pavlović ◽  
Marcin Nowicki ◽  
Sarah L. Boggess ◽  
Robert N. Trigiano
Keyword(s):  
Endangered Species ◽  
Shoot Proliferation ◽  
Genetic Fidelity ◽  
Induction Medium ◽  
Axillary Shoot ◽  
Axillary Shoot Proliferation ◽  
Regenerated Plants ◽  
Micropropagated Plants ◽  
Ssrs Markers

Helianthus verticillatus (Asteraceae), whorled sunflower, is a perennial species restricted to a few locations in the Southeastern United States. Habitat loss has caused H. verticillatus to become rare, and since 2014, it has been federally listed as an endangered species. As a part of the recovery plan for the restoration and protection of H. verticillatus, an efficient micropropagation protocol based on axillary shoot proliferation was developed. Various concentrations of 6-benzylaminopurine (BAP; 0 to 4.44 µM) were examined for their morphogenetic potential in the regeneration of six genotypes of H. verticillatus from the nodal explants derived from greenhouse-grown plants. Both the BAP concentration and genotype had significant effects on the regeneration capacity of H. verticillatus. Although the induced buds were observed on ½-strength Murashige and Skoog medium without plant growth regulators, a higher rate of induction and bud development were achieved on media with either 0.88 or 2.22 µM BAP, regardless of the genotype. Successful rooting of the induced shoots was achieved within four weeks after the transfer from the induction medium to the fresh ½-strength MS medium, but the rooting efficiency was dependent on the plant’s genetic background. Regenerated plantlets, with well-developed shoots and roots, were acclimatized successfully to greenhouse conditions with a 97% survival rate. Simple sequence repeats (SSRs) markers were employed to assess the genetic uniformity of the micropropagated plants of H. verticillatus. No extraneous bands were detected between regenerants and their respective donor plants, confirming the genetic fidelity and stability of regenerated plants. To our knowledge, the protocol developed in this study is the first such report for this endangered species.

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