Cytotoxic effect of nanoparticles synthesized from Salvia officinalis L. and Ricinus communis aqueous extracts against vero cell line and evaluation of their antioxidant activities

2012 ◽  
Vol 11 (52) ◽  
Author(s):  
Abid Nabil Ben Salem,
Keyword(s):  
Vero Cell ◽  
Cell Line ◽  
Cytotoxic Effect ◽  
Antioxidant Activities ◽  
Ricinus Communis ◽  
Salvia Officinalis ◽  
Aqueous Extracts ◽  
Vero Cell Line ◽  
Salvia Officinalis L

scholarly journals CYTOTOXICITY IDENTIFICATION OF THE SUPERNATANT OF Manihot esculenta Crantz RHIZOME AS RIBOSOME-INACTIVATING PROTEIN TO NORMAL CELL

2010 ◽  
Vol 4 (2) ◽  
pp. 77-81
Author(s):  
Endang Astuti ◽  
Sabirin Matsjeh ◽  
Winarto Haryadi ◽  
Deni Pranowo ◽  
Nuning Sri Mulatsih
Keyword(s):  
Cytotoxic Activity ◽  
Vero Cell ◽  
Cell Line ◽  
Cancer Cell ◽  
Manihot Esculenta ◽  
Normal Cell ◽  
Cytotoxic Effect ◽  
Ribosome Inactivating Protein ◽  
Manihot Esculenta Crantz ◽  
Vero Cell Line

The supernatant of Manihot esculenta Crantz rhizome has been used as an alternative remedy of infectious and cancer in Jogjakarta. Cytotoxicity assay showed that  Manihot esculenta Crantz supernatant had cytotoxic effect to cancer cell line, namely Myeloma (LC50 = 180,24 μg/mL) and HeLa (LC50 = 415,55 μg/mL), but have a little cytotoxic effect to SiHa. This research was aimed to identify cytotoxic activity of Manihot esculenta Crantz's supernatant to normal cell, particularly to human mononuclear cell and Vero cell line, and to compare to the cancer cell lines. The result showed that supernatant of M. esculenta had cytotoxic effect to normal mononuclear cell (LC50 = 564,00 μg/mL) and Vero cell line (LC50 = 686,00 μg/mL). The supernatant of M. esculenta had the highest cytotoxic activity to myeloma and relatively toxic to cervix cancer HeLa and normal cell, but less to SiHa.   Keywords: Manihot esculenta Crantz, Ribosome-inactivating Protein (RIP), cytotoxic, normal cell, cancer cell

scholarly journals Antibacterial effect and cytotoxic effect of supercritical extraction of thyme and thyme extract obtained through hydrodistillation process

2009 ◽  
Vol 63 (1-2) ◽  
pp. 17-27 ◽  
Author(s):  
Dusan Misic ◽  
Jakov Nisavic ◽  
Irena Zizovic ◽  
Jasna Ivanovic
Keyword(s):  
Vero Cell ◽  
Cell Line ◽  
Cytotoxic Effect ◽  
Antibacterial Effect ◽  
Supercritical Extraction ◽  
Vero Cell Line ◽  
Reference Strains

Investigations of antibacterial effect of supercritical extraction of thyme (TNKE) and thyme extract obtained by hydrodistillation (THD) covered 18 bacteria strains from the genuses Staphylococcus, Streptococcus and Enterococcus, including the strains MRSA, the strains VRE, as well as the reference strains S.pyogenes ATCC 19615, S. agalactiae ATCC 27959, S. aureus ATCC 11632, and S.aureus ATCC 25923. The anibacterial effect of thyme extracts was examined using the microdillution method in bouillon, and the cytotoxic effect of thyme extract was examined on the VERO cell line. The obtained values for MIC THD extract ranged from 160?g/mL to 1280?g/mL for all bacteria strains covered by the investigations. The obtained values for MIC TNKE extract were 1280 ?g/mL to 2560 ?g/mL for all examined strains except for 1 strain of S. intermedius of 320 ?g/mL. The examined extracts revealed a cytotoxic effect on the VERO cell line in concentrations higher than 40 ?g/mL.

Whole Genome Sequencing and Phylogenetic Analysis of the Rabies Virus Strain Moscow 3253 Adapted to a Vero Cell Line

2020 ◽  
Vol 35 (4) ◽  
pp. 237-242
Author(s):  
Ya. M. Krasnov ◽  
Zh. V. Alkhova ◽  
S. V. Generalov ◽  
I. V. Tuchkov ◽  
E. A. Naryshkina ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Vero Cell ◽  
Whole Genome Sequencing ◽  
Cell Line ◽  
Rabies Virus ◽  
Genome Sequencing ◽  
Virus Strain ◽  
Whole Genome ◽  
Vero Cell Line ◽  
Rabies Virus Strain

scholarly journals Antibacterial Activity, Cytotoxicity, and the Mechanism of Action of Bacteriocin from Bacillus subtilis GAS101

2018 ◽  
Vol 27 (2) ◽  
pp. 186-192 ◽  
Author(s):  
Garima Sharma ◽  
Shweta Dang ◽  
Sanjay Gupta ◽  
Reema Gabrani
Keyword(s):  
Molecular Weight ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Vero Cell ◽  
Cell Line ◽  
Mode Of Action ◽  
Proteolytic Enzymes ◽  
Antibiofilm Activity ◽  
Vero Cell Line ◽  
Soil Isolate

Objective: The aim of this study was to purify and characterize bacteriocin from the soil isolate Bacillus subtilis GAS101, and to determine its antimicrobial as well as antibiofilm potential. The purified bacteriocin was further analyzed and evaluated for mammalian cell cytotoxicity and the possible mode of action. Material and Methods: Bacteriocin from B. subtilis GAS101 (an animal husbandry soil isolate) was partially purified and checked for antimicrobial and antibiofilm activity against gram-positive and gram-negative bacteria. The molecular weight of bacteriocin was determined using tricine SDS-PAGE gel. The stability of bacteriocin was investigated at various temperatures and pH levels, and its sensitivity towards 8 enzymes and 6 chemicals was determined. Cytotoxicity analysis was performed on a Vero cell line by a tetrazolium dye-based assay. Scanning electron microscopy (SEM) of bacteriocin-treated bacteria was carried out to determine the possible mode of action. Results: Bacteriocin from B. subtilis GAS101 was a potential inhibitor of both the indicator organisms (Staphylococcus epidermidis and Escherichia coli), and had a molecular weight of approximately 6.5 kDa. An in situ gel assay showed a zone of inhibition corresponding to the estimated protein band size. Bacteriocin was stable and showed antibacterial activity in broad ranges of temperature (30–121°C) and pH (2–12). It was sensitive to 4 proteolytic enzymes, which indicated its proteinaceous nature. Bacteriocin showed > 70% cell viability on the mammalian Vero cell line. SEM depicted that the bacteriocin was able to disrupt the bacterial cell membrane as its probable mode of action. Conclusion: Thermostable and pH-tolerant bacteriocin from B. subtilis GAS101, of about 6.5 kDa, showed broad-spectrum antimicrobial and antibiofilm activity.

Live cold-adapted influenza A vaccine produced in Vero cell line

2004 ◽  
Vol 103 (1-2) ◽  
pp. 187-193 ◽  
Author(s):  
Julia Romanova ◽  
Dietmar Katinger ◽  
Boris Ferko ◽  
Brigitta Vcelar ◽  
Sabine Sereinig ◽  
...  
Keyword(s):  
Vero Cell ◽  
Cell Line ◽  
Influenza A ◽  
Cold Adapted ◽  
Vero Cell Line

scholarly journals Antioxidant and Proapoptotic Activities ofSclerocarya birrea[(A. Rich.) Hochst.] Methanolic Root Extract on the Hepatocellular Carcinoma Cell Line HepG2

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Maria Francesca Armentano ◽  
Faustino Bisaccia ◽  
Rocchina Miglionico ◽  
Daniela Russo ◽  
Nicoletta Nolfi ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
Hepg2 Cells ◽  
Cytotoxic Effect ◽  
Antioxidant Activities ◽  
Dermal Fibroblasts ◽  
Root Extract ◽  
Dependent Manner ◽  
Mitochondrial Membrane Depolarization

The main goal of this study was to characterize thein vitroantioxidant activity and the apoptotic potential ofS. birreamethanolic root extract (MRE). Among four tested extracts, obtained with different solvents, MRE showed the highest content of polyphenols, flavonoids, and tannins together with antioxidant activities tested with superoxide, nitric oxide, ABTS, and beta-carotene bleaching assays. Moreover, the cytotoxic effect of MRE was evaluated on the hepatocarcinoma cell line HepG2. In these cells, MRE treatment induced apoptosis and generated reactive oxygen species (ROS) in dose-dependent manner. The cytotoxic effect promoted by MRE was prevented by pretreatment of HepG2 cells with N-acetyl-L-cysteine (NAC), suggesting that oxidative stress was pivotal in MRE-mediated cell death. Moreover, we showed that the MRE treatment induced the mitochondrial membrane depolarization and the cytochromecrelease from mitochondria into the cytosol. It suggests that the apoptosis occurred in a mitochondrial-dependent pathway. Interestingly, MRE showed a sensibly lower cytotoxicity, associated with a low increase of ROS, in normal human dermal fibroblasts compared to HepG2 cells. It is suggested that the methanolic root extract ofS. Birreais able to selectively increase intracellular ROS levels in cancer cells, promoting cell death.

Drought effects on polyphenol composition and antioxidant activities in aerial parts of Salvia officinalis L.

2010 ◽  
Vol 33 (4) ◽  
pp. 1103-1111 ◽  
Author(s):  
Iness Bettaieb ◽  
Ibtissem Hamrouni-Sellami ◽  
Soumaya Bourgou ◽  
Ferid Limam ◽  
Brahim Marzouk
Keyword(s):  
Antioxidant Activities ◽  
Salvia Officinalis ◽  
Aerial Parts ◽  
Salvia Officinalis L ◽  
Drought Effects
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