scholarly journals Accelerated storage testing of freeze-dried Pseudomonas fluorescens BTP1, BB2 and PI9 strains

2012 ◽  
Vol 11 (95) ◽  
pp. 16187-16191 ◽  
Author(s):  
Kanyinda Jean Noeuml l Mputu ◽  
Jacqueline Destain ◽  
Philippe Noki ◽  
Philippe Thonart
Keyword(s):  
Pseudomonas Fluorescens ◽  
Accelerated Storage ◽  
Freeze Dried

Growth of Listeria monocytogenes Restricted by Native Microorganisms and Other Properties of Fresh-Cut Spinach†

1997 ◽  
Vol 60 (8) ◽  
pp. 912-917 ◽  
Author(s):  
ISABELLE BABIC ◽  
A. E. WATADA ◽  
J. G. BUTA
Keyword(s):  
Listeria Monocytogenes ◽  
Pseudomonas Fluorescens ◽  
Inhibitory Effect ◽  
Mixed Cultures ◽  
Bacteriostatic Activity ◽  
Staphylococcus Xylosus ◽  
Freeze Dried ◽  
Fresh Cut ◽  
Spinach Leaves

A study was undertaken to investigate the cause of the bacteriostatic activity of fresh-cut spinach leaves against Listeria monocytogenes. L. monocytogenes was cultivated in pure tryptic soy broth for use as a monoculture, in tryptic soy broth containing 10 mg ml−1 of autoclaved or nonautoclaved freeze-dried spinach powder, and in tryptic soy broth in mixed cultures with various microorganisms isolated from fresh-cut spinach, including Pseudomonas fluorescens biovar I, P. fluorescens biovar III, Staphylococcus xylosus, and an undefined culture of mesophilic aerobic microorganisms (MAMs) isolated from freeze-dried spinach powder. These microorganisms were inoculated at 4.4 log CFU ml−1 and L. monocytogenes was inoculated at 2.4 and 4.4 log CFU ml−1 After 24 h of incubation at 30°C, the populations of the two inoculum levels L. monocytogenes increased to 9.0 and 9.6 log CFU ml−1 in the tryptic soy broth control, to 5.4 and 7.5 in nonautoclaved spinach powder cultures, and to 8.8 and 9.1 log CFU ml−1 in autoclaved spinach powder cultures; In mixed cultures with biovar I of P.fluorescens, L. monocytogenes increased to 7.4 and 8.6 log CFU ml−1; with biovar III to 7.7 and 9.1, with S. xylosus to 7.8 and 9.2, and with the MAMs to 7.1 and 8.0 CFU ml−1 in the low and high listerial inoculum cultures respectively. The LSD(0.05)of the means were 0.5 and 0.6, respectively. The freeze-dried spinach powder had an inhibitory effect on the growth of L. monocytogenes. The inhibitory effect was greatly decreased when the native microorganisms were almost eliminated by heating or irradiation. These results indicate that if L. monocytogenes is present as a contaminant on fresh-cut spinach, its growth probably will be restricted by native microorganisms.

Aldehyde reactivity with 2-thiobarbituric acid and TBARS in freeze-dried beef during accelerated storage

Meat Science ◽  
2001 ◽  
Vol 57 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Q Sun ◽  
C Faustman ◽  
A Senecal ◽  
A.L Wilkinson ◽  
H Furr
Keyword(s):  
Thiobarbituric Acid ◽  
Accelerated Storage ◽  
Freeze Dried
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