Dependence of catalytic performance of a freeze-dried whole-cell biocatalyst of Pseudomonas fluorescens in regioselective acetylation of 1-β-d-arabinofuranosylcytosine on growth conditions

2012 ◽  
Vol 47 (12) ◽  
pp. 1826-1831 ◽  
Author(s):  
Xiao-Feng Li ◽  
Zhi-Hong Lu ◽  
Hong-Wei Liu ◽  
Guang-Lei Zhao ◽  
Hui Wu ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Growth Conditions ◽  
Catalytic Performance ◽  
Whole Cell ◽  
Freeze Dried ◽  
Whole Cell Biocatalyst

Catalytic Performance of a Robust Whole-Cell Biocatalyst in the Regioselective Synthesis of Helicid Esters Under Optimized Processing Conditions

2020 ◽  
Vol 150 (7) ◽  
pp. 1841-1848 ◽  
Author(s):  
Ting-Ting Wu ◽  
Xiang-Jie Zhao ◽  
Rong-Ling Yang ◽  
Muhammad Bilal ◽  
Zhao-Yu Wang ◽  
...  
Keyword(s):  
Catalytic Performance ◽  
Regioselective Synthesis ◽  
Processing Conditions ◽  
Whole Cell ◽  
Whole Cell Biocatalyst

scholarly journals Tuning a bi-enzymatic cascade reaction in Escherichia coli to facilitate NADPH regeneration for ε-caprolactone production

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jinghui Xiong ◽  
Hefeng Chen ◽  
Ran Liu ◽  
Hao Yu ◽  
Min Zhuo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Binding Sites ◽  
Regeneration System ◽  
Nadph Regeneration ◽  
Whole Cell ◽  
Cyclohexanone Monooxygenase ◽  
Ribosome Binding ◽  
Ribosome Binding Sites ◽  
Whole Cell Biocatalyst ◽  
Substrate Tolerance

Abstractε-Caprolactone is a monomer of poly(ε-caprolactone) which has been widely used in tissue engineering due to its biodegradability and biocompatibility. To meet the massive demand for this monomer, an efficient whole-cell biocatalytic approach was constructed to boost the ε-caprolactone production using cyclohexanol as substrate. Combining an alcohol dehydrogenase (ADH) with a cyclohexanone monooxygenase (CHMO) in Escherichia coli, a self-sufficient NADPH-cofactor regeneration system was obtained. Furthermore, some improved variants with the better substrate tolerance and higher catalytic ability to ε-caprolactone production were designed by regulating the ribosome binding sites. The best mutant strain exhibited an ε-caprolactone yield of 0.80 mol/mol using 60 mM cyclohexanol as substrate, while the starting strain only got a conversion of 0.38 mol/mol when 20 mM cyclohexanol was supplemented. The engineered whole-cell biocatalyst was used in four sequential batches to achieve a production of 126 mM ε-caprolactone with a high molar yield of 0.78 mol/mol.

Antityrosinase Mechanism and Antimelanogenic Effect of Arbutin Esters Synthesis Catalyzed by Whole-Cell Biocatalyst

2021 ◽  
Vol 69 (14) ◽  
pp. 4243-4252
Author(s):  
Haixia Xu ◽  
Xiaofeng Li ◽  
Xuan Xin ◽  
Lan Mo ◽  
Yucong Zou ◽  
...  
Keyword(s):  
Whole Cell ◽  
Whole Cell Biocatalyst

Engineered whole-cell biocatalyst-based detoxification and detection of neurotoxic organophosphate compounds

2014 ◽  
Vol 32 (3) ◽  
pp. 652-662 ◽  
Author(s):  
Chang Sup Kim ◽  
Jeong Hyun Seo ◽  
Dong Gyun Kang ◽  
Hyung Joon Cha
Keyword(s):  
Whole Cell ◽  
Organophosphate Compounds ◽  
Whole Cell Biocatalyst

Production of biodiesel by immobilized whole-cell biocatalyst

2008 ◽  
Vol 136 ◽  
pp. S385-S386
Author(s):  
Jyh-Ping Chen ◽  
Shu-Chin Chang
Keyword(s):  
Whole Cell ◽  
Whole Cell Biocatalyst

scholarly journals Accelerated storage testing of freeze-dried Pseudomonas fluorescens BTP1, BB2 and PI9 strains

2012 ◽  
Vol 11 (95) ◽  
pp. 16187-16191 ◽  
Author(s):  
Kanyinda Jean Noeuml l Mputu ◽  
Jacqueline Destain ◽  
Philippe Noki ◽  
Philippe Thonart
Keyword(s):  
Pseudomonas Fluorescens ◽  
Accelerated Storage ◽  
Freeze Dried

Permeabilizing Escherichia coli for whole cell biocatalyst with enhanced biotransformation ability from l-glutamate to GABA

2014 ◽  
Vol 107 ◽  
pp. 39-46 ◽  
Author(s):  
Wei-rui Zhao ◽  
Jun Huang ◽  
Chun-long Peng ◽  
Sheng Hu ◽  
Pi-yu Ke ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Whole Cell ◽  
Whole Cell Biocatalyst
Sign in / Sign up

Export Citation Format

Share Document