Study on the triphenyl tetrazolium chloride–dehydrogenase activity (TTC-DHA) method in determination of bioactivity for treating tomato paste wastewater

2012 ◽  
Vol 11 (27) ◽  
Author(s):  
Shiyang Sun
Keyword(s):  
Dehydrogenase Activity ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
Tomato Paste

scholarly journals Potential Fertilization Capacity of Two Grapevine Varieties: Effects on Agricultural Production in Designation of Origin Areas in the Northwestern Iberian Peninsula

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 961
Author(s):  
J. Antonio Cortiñas Rodríguez ◽  
María Fernández-González ◽  
Estefanía González-Fernández ◽  
Rosa A. Vázquez-Ruiz ◽  
F. Javier Rodríguez-Rajo ◽  
...  
Keyword(s):  
Airborne Pollen ◽  
Germination Rate ◽  
Pollen Viability ◽  
Weather Conditions ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
The Mean ◽  
Study Sites ◽  
Fertilization Capacity

In the present study, we analyzed the main parameters related with the potential fertilization ability of two grapevine varieties, Godello and Mencía, during the years 2017 and 2018. The research was carried out in two vineyards of the Galician winegrowing Designation of Origin areas of Ribeiro and Ribeira Sacra. Ten vines of each variety were selected for bunch and flower counting, pollen calculations, pollen viability studies by means of aceto-carmine (AC) stain and 2, 3, 5-triphenyl tetrazolium chloride (TTC) methods, and the determination of their germination rate. In all vineyards the 50% fruitset was reached, except for Godello in Cenlle during 2017. The mean coulure value was higher for Godello (40.5%) than for Mencía (31%). Analyzing the pollen production per plant and airborne pollen levels, we observed important discordances between them, which can be due to the influence of weather conditions and be related with self-pollination processes. We found important differences on pollen viability depending on the applied method and variety, with higher values for the AC method than the TTC for both varieties in all study plots, and higher values for Mencía variety than Godello. Regarding germination rates, we observed a marked reduction in 2017 with respect to 2018, in all study sites and for both varieties. The analyzed parameters were useful to explain the different productive abilities of Godello and Mencía varieties in the two studied bioclimatic regions of Ribeiro and Ribeira Sacra.

A Histochemical Study of Dehydrogenase Activity in the Pectoralis Major Muscle of the Pigeon and certain other Vertebrate Skeletal Muscles

1958 ◽  
Vol s3-99 (48) ◽  
pp. 469-473
Author(s):  
J. C. GEORGE ◽  
K. S. SCARIA
Keyword(s):  
Dehydrogenase Activity ◽  
Skeletal Muscles ◽  
Pectoralis Major Muscle ◽  
Breast Muscle ◽  
Muscle Fibres ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
Unique System ◽  
Oxidative Processes ◽  
The Individual

Certain dehydrogenases in the breast muscle of the pigeon and fowl and the leg muscle of the fowl and frog were studied histochemically by the use of 2:3:5: triphenyl tetrazolium chloride. The dehydrogenase activity was found to have a relationship with the colour and the mitochondrial content of the individual muscle fibres. In the pigeon breast muscle, however, the broad white fibres did not show the presence of any of the enzymes studied. It is therefore concluded that these fibres in the pigeon breast muscle are a unique system in which none of the oxidative processes concerned takes place; they cannot be considered as analogous to the white fibres of the other muscles studied.

scholarly journals Evaluation of freezing injury in temperate fruit trees

2020 ◽  
Vol 61 (5) ◽  
pp. 787-794
Author(s):  
Duk Jun Yu ◽  
Hee Jae Lee
Keyword(s):  
Fruit Trees ◽  
Freezing Stress ◽  
Freezing Injury ◽  
Triphenyl Tetrazolium Chloride ◽  
Natural Conditions ◽  
Visual Evaluation ◽  
Tetrazolium Chloride ◽  
Cultural Strategies ◽  
Analysis Determination

Abstract Freezing is a major environmental stress limiting the geographical distribution, growth, and productivity of temperate fruit trees. The extent of freezing injury in the trees depends on the rate at which the temperature decreases, the minimum temperature reached, and the duration of the freezing conditions. The ability to tolerate freezing temperatures under natural conditions varies greatly among fruit tree species, cultivars, and tissues. Freezing injury must be precisely evaluated to reliably predict the winter survival and productivity of the trees in specific regions, to screen for tolerant species and cultivars, and to develop cultural strategies that reduce freezing stress. Various methods are used to evaluate freezing injury in temperate fruit trees under field and artificial conditions, including visual evaluation of tissue discoloration, thermal analysis, determination of electrolyte leakage, and triphenyl tetrazolium chloride reduction analysis. In this review, we describe the most frequently used experimental procedures for evaluating freezing injury.

Valine, malic, and pyruvic dehydrogenase tests in the differentiation of Bacteroides

1975 ◽  
Vol 21 (9) ◽  
pp. 1369-1371
Author(s):  
Noel R. Funderburk ◽  
A. S. Kester
Keyword(s):  
Amino Acids ◽  
Organic Acids ◽  
Dehydrogenase Activity ◽  
Rapid Method ◽  
Pyruvic Acid ◽  
Triphenyl Tetrazolium Chloride ◽  
Hydrogen Acceptor ◽  
Tetrazolium Chloride

Fifteen isolates of Bacteroides were tested for their ability to dehydrogenate a variety of amino acids and organic acids. A simple and rapid method was developed for detecting dehydrogenase activity using 2,3,5-triphenyl tetrazolium chloride as a hydrogen acceptor and indicator. The results indicate that the tests for valine, malic, and pyruvic acid dehydrogenases have value in differentiating organisms in this genus.

scholarly journals Determination of Xanthine Oxidase in Milk with Triphenyl Tetrazolium Chloride

1956 ◽  
Vol 39 (5) ◽  
pp. 522-527 ◽  
Author(s):  
C.A. Zittle ◽  
E.S. Dellamonica ◽  
J.H. Custer ◽  
R.K. Rudd
Keyword(s):  
Xanthine Oxidase ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride

scholarly journals Biometric characterization and tetrazolium test in Campomanesia phaea O. Berg. Landrum seeds

2021 ◽  
Vol 43 ◽  
Author(s):  
Aparecida Leonir da Silva ◽  
Mariane Jeronimo Forte ◽  
Angelo Pedro Jacomino ◽  
Victor Augusto Forti ◽  
Simone Rodrigues da Silva
Keyword(s):  
Water Content ◽  
Chloride Salt ◽  
Triphenyl Tetrazolium Chloride ◽  
Seed Length ◽  
Tetrazolium Chloride ◽  
Content Determination ◽  
Water Contents ◽  
Nutritional Potential ◽  
Tetrazolium Test

ABSTRACT: Campomanesia phaea has commercial interest because its fruits are used in the manufacture of several products, besides its high nutritional potential. Nevertheless, it has low germination potential and there is not much information in the literature. The aim of this study was to perform a biometric characterization and to establish the tetrazolium test for cambuci seeds. Seeds obtained from two collection periods were subjected to biometric evaluations (seed length, thickness, width and mass), water content, determination of the soaking curve and tetrazolium test. The soaking curve was performed with non-scarified seeds and seeds subjected to two scarification methods, mechanical and chemical. The tetrazolium test was performed soaking the seeds in a solution of 2, 3, 5 triphenyl tetrazolium chloride salt at 0.75, 1.00 and 1.50% for 4, 8 and 12 hours. After this period, the seeds were categorized as viable and non-viable. It was concluded that the cambuci seeds presented biometric differences between the two collection periods, because of the different water contents. There was no difference in the soaking curve among the three treatments tested and the solution of 0.75% for four hours in a water bath at 38 oC was considered the best for performing the tetrazolium test.

Determination of Choline Dehydrogenase Activity along the Rat Nephron

1996 ◽  
Vol 377 (2) ◽  
pp. 129-138 ◽  
Author(s):  
Bernhard Miller ◽  
Heide Schmid ◽  
Tian Jun Chen ◽  
Michael Schmolke ◽  
Walter G. Guder
Keyword(s):  
Dehydrogenase Activity ◽  
Choline Dehydrogenase
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