scholarly journals Effect of assay conditions on the measurement of dehydrogenase activity of <i>Streptomyces venezuelae</i> using triphenyl tetrazolium chloride

2011 ◽  
Vol 02 (04) ◽  
pp. 214-225 ◽  
Author(s):  
Tracey Burdock ◽  
Marianne Brooks ◽  
Abdel Ghaly ◽  
Deepika Dave
Keyword(s):  
Dehydrogenase Activity ◽  
Triphenyl Tetrazolium Chloride ◽  
Streptomyces Venezuelae ◽  
Tetrazolium Chloride ◽  
Assay Conditions

A Histochemical Study of Dehydrogenase Activity in the Pectoralis Major Muscle of the Pigeon and certain other Vertebrate Skeletal Muscles

1958 ◽  
Vol s3-99 (48) ◽  
pp. 469-473
Author(s):  
J. C. GEORGE ◽  
K. S. SCARIA
Keyword(s):  
Dehydrogenase Activity ◽  
Skeletal Muscles ◽  
Pectoralis Major Muscle ◽  
Breast Muscle ◽  
Muscle Fibres ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
Unique System ◽  
Oxidative Processes ◽  
The Individual

Certain dehydrogenases in the breast muscle of the pigeon and fowl and the leg muscle of the fowl and frog were studied histochemically by the use of 2:3:5: triphenyl tetrazolium chloride. The dehydrogenase activity was found to have a relationship with the colour and the mitochondrial content of the individual muscle fibres. In the pigeon breast muscle, however, the broad white fibres did not show the presence of any of the enzymes studied. It is therefore concluded that these fibres in the pigeon breast muscle are a unique system in which none of the oxidative processes concerned takes place; they cannot be considered as analogous to the white fibres of the other muscles studied.

Valine, malic, and pyruvic dehydrogenase tests in the differentiation of Bacteroides

1975 ◽  
Vol 21 (9) ◽  
pp. 1369-1371
Author(s):  
Noel R. Funderburk ◽  
A. S. Kester
Keyword(s):  
Amino Acids ◽  
Organic Acids ◽  
Dehydrogenase Activity ◽  
Rapid Method ◽  
Pyruvic Acid ◽  
Triphenyl Tetrazolium Chloride ◽  
Hydrogen Acceptor ◽  
Tetrazolium Chloride

Fifteen isolates of Bacteroides were tested for their ability to dehydrogenate a variety of amino acids and organic acids. A simple and rapid method was developed for detecting dehydrogenase activity using 2,3,5-triphenyl tetrazolium chloride as a hydrogen acceptor and indicator. The results indicate that the tests for valine, malic, and pyruvic acid dehydrogenases have value in differentiating organisms in this genus.

scholarly journals Potential Fertilization Capacity of Two Grapevine Varieties: Effects on Agricultural Production in Designation of Origin Areas in the Northwestern Iberian Peninsula

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 961
Author(s):  
J. Antonio Cortiñas Rodríguez ◽  
María Fernández-González ◽  
Estefanía González-Fernández ◽  
Rosa A. Vázquez-Ruiz ◽  
F. Javier Rodríguez-Rajo ◽  
...  
Keyword(s):  
Airborne Pollen ◽  
Germination Rate ◽  
Pollen Viability ◽  
Weather Conditions ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
The Mean ◽  
Study Sites ◽  
Fertilization Capacity

In the present study, we analyzed the main parameters related with the potential fertilization ability of two grapevine varieties, Godello and Mencía, during the years 2017 and 2018. The research was carried out in two vineyards of the Galician winegrowing Designation of Origin areas of Ribeiro and Ribeira Sacra. Ten vines of each variety were selected for bunch and flower counting, pollen calculations, pollen viability studies by means of aceto-carmine (AC) stain and 2, 3, 5-triphenyl tetrazolium chloride (TTC) methods, and the determination of their germination rate. In all vineyards the 50% fruitset was reached, except for Godello in Cenlle during 2017. The mean coulure value was higher for Godello (40.5%) than for Mencía (31%). Analyzing the pollen production per plant and airborne pollen levels, we observed important discordances between them, which can be due to the influence of weather conditions and be related with self-pollination processes. We found important differences on pollen viability depending on the applied method and variety, with higher values for the AC method than the TTC for both varieties in all study plots, and higher values for Mencía variety than Godello. Regarding germination rates, we observed a marked reduction in 2017 with respect to 2018, in all study sites and for both varieties. The analyzed parameters were useful to explain the different productive abilities of Godello and Mencía varieties in the two studied bioclimatic regions of Ribeiro and Ribeira Sacra.

Oxygen uptake and tetrazolium reduction during skin cycle of the mouse

1965 ◽  
Vol 209 (1) ◽  
pp. 231-235 ◽  
Author(s):  
Ira M. Dushoff ◽  
John Payne ◽  
Falls B. Hershey ◽  
Robert C. Donaldson
Keyword(s):  
Oxygen Consumption ◽  
Mouse Skin ◽  
Growth Cycle ◽  
Triphenyl Tetrazolium Chloride ◽  
Intact Skin ◽  
Tetrazolium Chloride ◽  
Quantitative Measurements ◽  
Close Relationship ◽  
Coefficient Of Regression ◽  
Tetrazolium Reduction

Quantitative measurements of oxygen consumption and triphenyl tetrazolium chloride reduction by intact skin were made during various stages of the skin cycle of the mouse. Values are highest during early anagen, when growth is most rapid: Qo2 is 2.47 ± 0.10 (mean ± se) and TTC is 537 ± 24.6. In late anagen the activities decrease 40–50%: Qo2 is 1.24 ± 0.10 and TTC is 315 ± 20.8. There is a close relationship between tetrazolium reduction and oxygen consumption during all phases of the growth cycle of mouse skin. The coefficient of regression of TTC on Qo2 is 163.2 ( P < .001).

scholarly journals Histochemical and Quantitative Studies of the In Vitro Dehydrogenase Activity of Spleen in Hypersplenism

Blood ◽  
1955 ◽  
Vol 10 (2) ◽  
pp. 145-153 ◽  
Author(s):  
MAURICE M. BLACK ◽  
JOSEPH A. PRESTON ◽  
FRANCIS D. SPEER ◽  
PHYLLIS BRENOWITZ
Keyword(s):  
Structural Feature ◽  
Dehydrogenase Activity ◽  
Tetrazolium Chloride ◽  
Histological Studies ◽  
Quantitative Measurements ◽  
Quantitative Studies ◽  
Presence And Absence ◽  
Made In

Abstract Tetrazolium chloride was used to visualize the in vitro dehydrogenase activity of splenic tissues from cases with and without hypersplenism. In addition, quantitative measurements of such activity were made in the presence and absence of fluoride, malonate and azide. The data indicate that spleen slices from cases of hypersplenism differed from the controls and were characterized by (a) increased endogenous dehydrogenase activity of the lymphocytes, particularly around the follicles, (b) a decreased azide stimulation in those cases having an initial thrombocytopenia which responded to splenectomy, and (c) a decreased fluoride stimulation in those cases having an initial leukopenia which responded to splenectomy. Routine histological studies confirmed other reports that a constant structural feature of the spleen in hypersplenism is a perifollicular lymphocytic rimming.

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