scholarly journals Genetic analysis of wild and cultivated germplasm of pigeonpea using random amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) markers

2013 ◽  
Vol 12 (40) ◽  
pp. 5823-5832 ◽  
Author(s):  
Walunjkar Babasaheb ◽  
Parihar Akarsh ◽  
Chaurasia Pratibha ◽  
Pachchigar K ◽  
M Chauhan R
Keyword(s):  
Genetic Analysis ◽  
Ssr Markers ◽  
Simple Sequence Repeats ◽  
Random Amplified Polymorphic Dna ◽  
Simple Sequence

scholarly journals Comparison of RAPD, ISSR and SSR markers in assessing genetic diversity among rye (Secale cereale L.) inbred lines

2010 ◽  
Vol 62 (1) ◽  
pp. 107-115 ◽  
Author(s):  
Beata Myśków ◽  
Paweł Milczarski ◽  
Piotr Masojć
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Secale Cereale ◽  
Simple Sequence Repeats ◽  
Random Amplified Polymorphic Dna ◽  
Correlation Coefficients ◽  
Inbred Lines ◽  
Winter Rye ◽  
Dna Primers ◽  
Simple Sequence

Comparison of RAPD, ISSR and SSR markers in assessing genetic diversity among rye (Secale cereale L.) inbred lines Forty eight inbred lines of winter rye, of various origin and pedigree, were analysed using 19 RAPD (random amplified polymorphic DNA) primers, 8 ISSR (inter-simple sequence repeats) primers and 13 SSR (simple sequence repeats) primer pairs. On the basis of particular marker types, there were created three separate dendrograms and one combined similarity tree, prepared on account of the whole data. Correlation coefficients for individual technique based on genetic similarity matrices were not significant. By comparing the GS data obtained on the basis of singular methods with collective matrix, it was observed that the highest correlation rate was for ISSR method (r=0.68). The utility of each marker technique was compared by using marker index MI. Diversity detecting index (DDT) was suggested in the paper, which may prove helpful in planning and comparing researches on phenetic relationships.

scholarly journals Development of EST-derived SSR Markers with Long-core Repeat in Olive and Their Use for Paternity Testing

2013 ◽  
Vol 138 (4) ◽  
pp. 290-296 ◽  
Author(s):  
Raúl De la Rosa ◽  
Angjelina Belaj ◽  
Antonio Muñoz-Mérida ◽  
Oswaldo Trelles ◽  
Inmaculada Ortíz-Martín ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Simple Sequence Repeats ◽  
Expressed Sequence Tag ◽  
Paternity Testing ◽  
High Level ◽  
Repeated Motifs ◽  
Expressed Sequence ◽  
Simple Sequence ◽  
Olive Breeding ◽  
Genomic Project

In the present work, a set of eight new hexa-nucleotide simple sequence repeats (SSRs) is reported in olive (Olea europaea L). These SSRs loci were generated on the basis of expressed sequence tag (EST) sequences in the frame of an olive genomic project. The markers showed a high level of polymorphism when tested on a set of cultivars used as genitors in the olive breeding program of Córdoba, Spain. The long-core repeat motif of these markers allows a wider separation among alleles, thus permitting an accurate genotyping. Besides, these markers showed comparable levels of polymorphism to di-nucleotide SSRs, the only ones so far reported in olive. Selected on the basis of their discrimination capacity, four of the eight SSRs were used to test their ability for paternity testing in a total of 81 seedlings coming from 12 crosses. The paternity testing showed that seven crosses matched the alleged paternity and the remaining five were products of illicit pollinations. These results exactly matched with previous paternity testing performed with di-nucleotide SSR markers. These results demonstrate the usefulness of the developed hexa-nucleotide repeated motifs for checking the paternity of breeding progenies and suggest their use on variability studies.

scholarly journals The characterization of a new set of EST-derived simple sequence repeat (SSR) markers as a resource for the genetic analysis of Phaseolus vulgaris

BMC Genetics ◽  
2011 ◽  
Vol 12 (1) ◽  
pp. 41 ◽  
Author(s):  
Robertha AV Garcia ◽  
Priscila N Rangel ◽  
Claudio Brondani ◽  
Wellington S Martins ◽  
Leonardo C Melo ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Genetic Analysis ◽  
Ssr Markers ◽  
Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Simple Sequence
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