Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme

2011 ◽  
Vol 10 (81) ◽  
Author(s):  
Ma’an Alkhatib
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Empty Fruit Bunch ◽  
Cellulase Enzyme ◽  
Immobilized Cellulase ◽  
Hydrolysis Of

scholarly journals Characterization of Residual Lignin Obtained by the Enzymatic Hydrolysis of Oil Palm Empty Fruit Bunch Pulps

BioResources ◽  
2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Yin Ying H'ng ◽  
Akiko Nakagawa-Izumi ◽  
Cheu Peng Leh ◽  
Atanu Kumar Das ◽  
Hiroshi Ohi
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Empty Fruit Bunch ◽  
Residual Lignin ◽  
Hydrolysis Of

scholarly journals Effect of Ultrasound-Assisted on Sequential Peracetic Acid and Alkaline Peroxide Pretreatment to The Enzymatic Hydrolysis of Oil Palm Empty Fruit Bunch

2021 ◽  
Author(s):  
Dwini Normayulisa Putri ◽  
Meka Saima Perdani ◽  
Masafumi Yohda ◽  
Tania Surya Utami ◽  
Muhamad Sahlan ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Peracetic Acid ◽  
Enzyme Concentration ◽  
Reducing Sugar ◽  
Empty Fruit Bunch ◽  
Hydrolysis Time ◽  
Alkaline Peroxide ◽  
Hydrolysis Process ◽  
Hydrolysis Of

Abstract Enzymatic hydrolysis of oil palm empty fruit bunch (OPEFB) that has been pretreated by modified pretreatment has been investigated in this study. The OPEFB used was pretreated by using sequential peracetic acid – alkaline peroxide solution. As the modification method, the assistance of pretreatment by ultrasound was conducted, in order to increase the enzyme accessibility. Therefore, it enhances the production of reducing sugar on the hydrolysis process. Prior to hydrolysis process, OPEFB was initially treated by using peracetic acid solution, comprise of CH3COOH (> 99%) and H2O2 (30% w/w), assisted by ultrasound for 3 hours at 35oC. Afterwards, OPEFB was treated by using alkaline peroxide solution, comprise of NaOH (40% w/w) and H2O2 (35% w/w), assisted by ultrasound for 10 hours at 35oC. OPEFB that has been pretreated was then subjected to enzymatic hydrolysis process using cellulase enzyme, in order to convert cellulose content into reducing sugar. Enzymatic hydrolysis was carried out at 50oC in a shaker incubator with 150 rpm for 48 hours. In this study, the effect of different enzyme concentration and hydrolysis time towards the sugar concentration in modified-pretreated OPEFB was observed and analyzed. Three different concentrations of enzyme were used, including 1.25, 2.5, and 5 g/L, and reducing sugar concentrations were analyzed at 30 and 45 minutes, and 1, 2, 4, 6, 24, 30, and 48 hours. Based on results, enzyme concentration has a significant effect to the production of reducing sugar. The reducing sugar concentrations obtained at the end of the hydrolysis process were 8.48, 11.06, 19.16 g/L, at the enzyme concentrations of 1.25, 2.5, and 5 g/L, respectively. At any hydrolysis time, the highest sugar concentration has been achieved on the highest enzyme concentration of 5 g/L. Moreover, the effective hydrolysis time were achieved at 6 hours, at all concentration of enzyme, since the production of reducing sugar were insignificant after 6 hours. This study showed an increase in reducing sugar production by 8.25% in the hydrolysis process using OPEFB pretreated by modified pretreatment compared to the non-modified pretreatment.

A KINETIC STUDY OF ENZYMATIC HYDROLYSIS OF OIL PALM BIOMASS FOR FERMENTABLE SUGAR USING POLYETHYLENE GLYCOL (PEG) IMMOBILIZED CELLULASE

2016 ◽  
Vol 78 (8-3) ◽  
Author(s):  
Umi Aisah Asli ◽  
Isah Nwaha ◽  
Hazirah Hamid ◽  
Zainul Akmar Zakaria ◽  
Aziatul Niza Sadikin ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Enzymatic Hydrolysis ◽  
Kinetic Study ◽  
Oil Palm ◽  
Fermentable Sugar ◽  
Michaelis Constant ◽  
Immobilized Cellulase ◽  
Free Interaction ◽  
Limiting Velocity ◽  
Hydrolysis Of

In this work, enzymatic hydrolysis by cellulase in a soluble and an immobilized form was studied to convert lignocellulosic oil palm empty fruit bunch (EFB) biomass into fermentable sugars as a feedstock for bioethanol production. The cellulase was covalently immobilized with activated and functionalized polyethylene glycol (PEG) via glutaraldehyde coupling method. As a whole, the immobilized cellulase displayed 50% higher efficiency over free cellulase, in reducing sugar recovery during hydrolysis reactions at pH of 4.8 and temperature of 50°C. From the kinetic study, it showed that Michaelis constant (Km) and limiting velocity (Vmax) of immobilized cellulase were 179.2 mg/ml and 33.5 mg/ml.min respectively, comparable with the value for free cellulose, 171.8 mg/ml and 34.5 mg/ml.min respectively. This result could be attributed to the effect of PEG on the binding cellulase to substrate desorb substrates, and enables free interaction of cellulase to hydrolyse cellulose maximally

Immobilized Nanoparticles-Mediated Enzymatic Hydrolysis of Cellulose for Clean Sugar Production: A Novel Approach

2019 ◽  
Vol 15 (3) ◽  
pp. 296-303 ◽  
Author(s):  
Swapnil Gaikwad ◽  
Avinash P. Ingle ◽  
Silvio Silverio da Silva ◽  
Mahendra Rai
Keyword(s):  
Catalytic Activity ◽  
Enzymatic Hydrolysis ◽  
Immobilized Enzyme ◽  
Free Enzyme ◽  
Magnetic Nanomaterials ◽  
Sugar Production ◽  
Cellulase Enzyme ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Background: Enzymatic hydrolysis of cellulose is an expensive approach due to the high cost of an enzyme involved in the process. The goal of the current study was to apply magnetic nanomaterials as a support for immobilization of enzyme, which helps in the repeated use of immobilized enzyme for hydrolysis to make the process cost-effective. In addition, it will also provide stability to enzyme and increase its catalytic activity. Objective: The main aim of the present study is to immobilize cellulase enzyme on Magnetic Nanoparticles (MNPs) in order to enable the enzyme to be re-used for clean sugar production from cellulose. Methods: MNPs were synthesized using chemical precipitation methods and characterized by different techniques. Further, cellulase enzyme was immobilized on MNPs and efficacy of free and immobilized cellulase for hydrolysis of cellulose was evaluated. Results: Enzymatic hydrolysis of cellulose by immobilized enzyme showed enhanced catalytic activity after 48 hours compared to free enzyme. In first cycle of hydrolysis, immobilized enzyme hydrolyzed the cellulose and produced 19.5 ± 0.15 gm/L of glucose after 48 hours. On the contrary, free enzyme produced only 13.7 ± 0.25 gm/L of glucose in 48 hours. Immobilized enzyme maintained its stability and produced 6.15 ± 0.15 and 3.03 ± 0.25 gm/L of glucose in second and third cycle, respectively after 48 hours. Conclusion: This study will be very useful for sugar production because of enzyme binding efficiency and admirable reusability of immobilized enzyme, which leads to the significant increase in production of sugar from cellulosic materials.

scholarly journals Enzymatic Hydrolysis of Pretreated Fibre Pressed Oil Palm Frond by using Sacchariseb C6

2017 ◽  
Vol 206 ◽  
pp. 012008
Author(s):  
F S Hashim ◽  
H W Yussof ◽  
M A K M Zahari ◽  
R A Rahman ◽  
R M Illias
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Oil Palm Frond ◽  
Hydrolysis Of ◽  
Palm Frond

Optimization studies on acid hydrolysis of oil palm empty fruit bunch fiber for production of xylose

2007 ◽  
Vol 98 (3) ◽  
pp. 554-559 ◽  
Author(s):  
S.H.A. Rahman ◽  
J.P. Choudhury ◽  
A.L. Ahmad ◽  
A.H. Kamaruddin
Keyword(s):  
Acid Hydrolysis ◽  
Oil Palm ◽  
Empty Fruit Bunch ◽  
Hydrolysis Of

scholarly journals Peningkatan Produksi Gula Pereduksi dari Tandan Kosong Kelapa Sawit dengan Praperlakuan Asam Organik pada Reaktor Bertekanan

REAKTOR ◽  
2017 ◽  
Vol 16 (4) ◽  
pp. 199
Author(s):  
Fahriya Puspita Sari ◽  
Nissa Nurfajrin Solihat ◽  
Sita Heris Anita ◽  
Fitria Fitria ◽  
Euis Hermiati
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oxalic Acid ◽  
Organic Acid ◽  
Oil Palm ◽  
Maleic Acid ◽  
Reducing Sugar ◽  
Sugar Production ◽  
Empty Fruit Bunch ◽  
Acid Pretreatment ◽  
Severity Factor

ENHANCEMENT OF REDUCING SUGAR PRODUCTION FROM OIL PALM EMPTY FRUIT BUNCH BY PRETREATMENT USING ORGANIC ACID IN PRESSURIZED REACTOR. Organic acids are potential to create more environmentally friendly process in the pretreatment of lignocellulosic biomass for bioethanol production. This study was aimed to investigate the influence of organic acid pretreatment in reducing sugar production in a pressurized reactor with various resident times and temperatures on enzymatic hydrolysis of OPEFB. Two different organic acids (maleic acid and oxalic acid) were used in the pretreatment of oil palm empty fruit bunch (OPEFB) using a pressurized reactor. Factorial design using three different temperatures (170, 180, and 190°C) and four resident times (15, 30, 45, and 60 min) were employed, followed by enzymatic hydrolysis. Each condition conducted two repetitions. Analysis was conducted on the reducing sugar that was produced after saccharification by means of the severity factor of each pretreatment condition. Maleic acid showed higher reducing sugar yield with lower severity factor than oxalic acid with the same operating conditions. The highest yield of reducing sugars (80.84%) was obtained using maleic acid at 170 for 60 minutes with severity factor of 1.836. Keywords: bioethanol; organic acid pretreatment; pressurized reactor; severity factor; oil palm empty fruit bunches;   Abstrak Asam organik berpotensi dalam membantu proses praperlakuan dari biomassa lignoselulosa untuk memproduksi bioetanol yang ramah lingkungan. Penelitian ini bertujuan untuk mengetahui pengaruh asam organik, suhu dan waktu operasi terhadap produksi gula pereduksi dengan reaktor bertekanan pada tandan kosong kelapa sawit. Dua asam organik yang berbeda yaitu asam oksalat dan asam maleat digunakan untuk proses praperlakuan tandan kosong kelapa sawit (TKKS) dengan bantuan reaktor bertekanan. Dalam proses praperlakuan digunakan tiga suhu yang berbeda yaitu suhu 170, 180, dan 190°C dan empat waktu operasi 15, 30, 45, dan 60 min yang dilanjutkan dengan proses hidrolisis enzimatis. Setiap kondisi dilakukan dua kali pengulangan. Analisa yang digunakan adalah analisa uji gula pereduksi dan severity factor pada kondisi tiap praperlakuan. Asam maleat menunjukkan hasil yang lebih baik dengan severity factor yang lebih rendah dibandingkan menggunakan asam oksalat dengan kondisi operasi yang sama. Hasil yang didapatkan menunjukkan bahwa praperlakuan tandan kosong kelapa sawit dengan bantuan reaktor bertekanan memiliki rendemen gula pereduksi optimum sebesar 80,84% dengan menggunakan asam maleat pada suhu 170°C selama 60 menit dengan severity factor sebesar 1,836. Kata kunci: bioetanol; praperlakuan asam organik; reaktor bertekanan; severity factor; tandan kosong kelapa sawit.

scholarly journals Enzymatic hydrolysis of oil palm empty fruit bunch to produce reducing sugar and its kinetic Hidrolisis enzimatik tandan kosong kelapa sawit untuk menghasilkan gula pereduksi dan kinetikanya

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Vera BARLIANTI ◽  
Deliana DAHNUM ◽  
. MURYANTO ◽  
Eka TRIWAHYUNI ◽  
Yosi ARISTIAWAN ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Volume Ratio ◽  
Enzyme Concentration ◽  
Reducing Sugar ◽  
Economic Feasibility ◽  
First Order ◽  
Empty Fruit Bunches ◽  
Hydrolysis Process ◽  
Hydrolysis Of

Abstrak Sebagai salah satu Negara penghasil minyak kelapa sawit mentah (CPO), Indonesia juga menghasilkan tandan kosong kelapa sawit (TKKS) dalam jumlah besar. TKKS terdiri dari-tiga-komponen utama, yaitu selulosa, hemiselulosa, dan lignin. Pengolahan awal TKKS secara alkalindi ikuti dengan hidrolisis TKKS secara enzimatik menggunakan kombinasi enzim selulase dan β-glukosidase akan menghasilkan gula-gula yang mudah difermentasi.  Penelitian ini bertujuan untuk mempelajari pengaruh konsentrasi substrat, kon-sentrasi enzim, dan suhu selama proses hidrolisis berlangsung.  Hasil yang diperoleh menunjukkan bahwa konsentrasi gula maksimum (194,78 g/L) dicapai pada konsentrasi TKKS 20% (b/v), konsentrasi campuran enzim yang terdiri dari selulase dan β-1,4 glukosidase sebesar 3,85% (v/v), dan suhu 50oC. Perbandingan antara selulase dan β-1,4 glukosidase adalah 5:1 dengan masing-masing aktivitas enzim sebesar 144.5 FPU/mL dan 63 FPU/mL. Hasil penelitian juga menunjukkan bahwa model kinetika yang sesuai untuk proses hidrolisis TKKS secara enzimatik adalah model kinetika Shen dan Agblevor dengan reakside aktivasi enzim orde satu.  Hasil ini mendukung studi kelayakan ekonomi dalam pemanfaatan TKKS untuk produksi bioetanol.AbstractAs one of the crude palm oil producers, Indonesia also produces empty fruit bunches (EFB)in large quantities. The oil palm EFB consist of cellulose, hemicellulose and lignin. Alkaline pretreatment of EFB, followed by enzymatic hydro-lysis of cellulose using combination of cellulase and β-glucosidase enzymes produce fermentable sugars. This paper reported the effects of substrate loading, enzyme concentration, and temperature of hydrolysis process on reducing sugar production. The  maximum  sugar  concentration (194.78 g/L) was produced at 50oC using 20% (w/v) EFB and 3.85% (v/v) mixed enzymes of cellulase and β-1,4 glucosidase in volume ratio of 5:1 (v/v), with enzyme activity of 144.5 FPU/mL and 63 FPU/mL, respectively. The results also showed that the suitable kinetic model for enzymatic hydrolysis process of oil palm EFB follow Shen and Agblevor model with first order of enzyme deactivation. These results support the economic feasibility study in utilization of EFB of oil palm for bioethanol production.    

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