A KINETIC STUDY OF ENZYMATIC HYDROLYSIS OF OIL PALM BIOMASS FOR FERMENTABLE SUGAR USING POLYETHYLENE GLYCOL (PEG) IMMOBILIZED CELLULASE

2016 ◽  
Vol 78 (8-3) ◽  
Author(s):  
Umi Aisah Asli ◽  
Isah Nwaha ◽  
Hazirah Hamid ◽  
Zainul Akmar Zakaria ◽  
Aziatul Niza Sadikin ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Enzymatic Hydrolysis ◽  
Kinetic Study ◽  
Oil Palm ◽  
Fermentable Sugar ◽  
Michaelis Constant ◽  
Immobilized Cellulase ◽  
Free Interaction ◽  
Limiting Velocity ◽  
Hydrolysis Of

In this work, enzymatic hydrolysis by cellulase in a soluble and an immobilized form was studied to convert lignocellulosic oil palm empty fruit bunch (EFB) biomass into fermentable sugars as a feedstock for bioethanol production. The cellulase was covalently immobilized with activated and functionalized polyethylene glycol (PEG) via glutaraldehyde coupling method. As a whole, the immobilized cellulase displayed 50% higher efficiency over free cellulase, in reducing sugar recovery during hydrolysis reactions at pH of 4.8 and temperature of 50°C. From the kinetic study, it showed that Michaelis constant (Km) and limiting velocity (Vmax) of immobilized cellulase were 179.2 mg/ml and 33.5 mg/ml.min respectively, comparable with the value for free cellulose, 171.8 mg/ml and 34.5 mg/ml.min respectively. This result could be attributed to the effect of PEG on the binding cellulase to substrate desorb substrates, and enables free interaction of cellulase to hydrolyse cellulose maximally

scholarly journals Enzymatic Hydrolysis of Pretreated Fibre Pressed Oil Palm Frond by using Sacchariseb C6

2017 ◽  
Vol 206 ◽  
pp. 012008
Author(s):  
F S Hashim ◽  
H W Yussof ◽  
M A K M Zahari ◽  
R A Rahman ◽  
R M Illias
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Oil Palm Frond ◽  
Hydrolysis Of ◽  
Palm Frond

scholarly journals Enzymatic hydrolysis of oil palm empty fruit bunch to produce reducing sugar and its kinetic Hidrolisis enzimatik tandan kosong kelapa sawit untuk menghasilkan gula pereduksi dan kinetikanya

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Vera BARLIANTI ◽  
Deliana DAHNUM ◽  
. MURYANTO ◽  
Eka TRIWAHYUNI ◽  
Yosi ARISTIAWAN ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Volume Ratio ◽  
Enzyme Concentration ◽  
Reducing Sugar ◽  
Economic Feasibility ◽  
First Order ◽  
Empty Fruit Bunches ◽  
Hydrolysis Process ◽  
Hydrolysis Of

Abstrak Sebagai salah satu Negara penghasil minyak kelapa sawit mentah (CPO), Indonesia juga menghasilkan tandan kosong kelapa sawit (TKKS) dalam jumlah besar. TKKS terdiri dari-tiga-komponen utama, yaitu selulosa, hemiselulosa, dan lignin. Pengolahan awal TKKS secara alkalindi ikuti dengan hidrolisis TKKS secara enzimatik menggunakan kombinasi enzim selulase dan β-glukosidase akan menghasilkan gula-gula yang mudah difermentasi.  Penelitian ini bertujuan untuk mempelajari pengaruh konsentrasi substrat, kon-sentrasi enzim, dan suhu selama proses hidrolisis berlangsung.  Hasil yang diperoleh menunjukkan bahwa konsentrasi gula maksimum (194,78 g/L) dicapai pada konsentrasi TKKS 20% (b/v), konsentrasi campuran enzim yang terdiri dari selulase dan β-1,4 glukosidase sebesar 3,85% (v/v), dan suhu 50oC. Perbandingan antara selulase dan β-1,4 glukosidase adalah 5:1 dengan masing-masing aktivitas enzim sebesar 144.5 FPU/mL dan 63 FPU/mL. Hasil penelitian juga menunjukkan bahwa model kinetika yang sesuai untuk proses hidrolisis TKKS secara enzimatik adalah model kinetika Shen dan Agblevor dengan reakside aktivasi enzim orde satu.  Hasil ini mendukung studi kelayakan ekonomi dalam pemanfaatan TKKS untuk produksi bioetanol.AbstractAs one of the crude palm oil producers, Indonesia also produces empty fruit bunches (EFB)in large quantities. The oil palm EFB consist of cellulose, hemicellulose and lignin. Alkaline pretreatment of EFB, followed by enzymatic hydro-lysis of cellulose using combination of cellulase and β-glucosidase enzymes produce fermentable sugars. This paper reported the effects of substrate loading, enzyme concentration, and temperature of hydrolysis process on reducing sugar production. The  maximum  sugar  concentration (194.78 g/L) was produced at 50oC using 20% (w/v) EFB and 3.85% (v/v) mixed enzymes of cellulase and β-1,4 glucosidase in volume ratio of 5:1 (v/v), with enzyme activity of 144.5 FPU/mL and 63 FPU/mL, respectively. The results also showed that the suitable kinetic model for enzymatic hydrolysis process of oil palm EFB follow Shen and Agblevor model with first order of enzyme deactivation. These results support the economic feasibility study in utilization of EFB of oil palm for bioethanol production.    

Synergistic effects on process parameters to enhance enzymatic hydrolysis of alkaline oil palm fronds

2018 ◽  
Vol 122 ◽  
pp. 617-626 ◽  
Author(s):  
Masniroszaime Md Zain ◽  
Abdul Wahab Mohammad ◽  
Shuhaida Harun ◽  
Nurul Aina Fauzi ◽  
Nur Hanis Hayati Hairom
Keyword(s):  
Enzymatic Hydrolysis ◽  
Process Parameters ◽  
Oil Palm ◽  
Synergistic Effects ◽  
Oil Palm Fronds ◽  
Palm Fronds ◽  
Hydrolysis Of

Dissecting the effect of polyethylene glycol on the enzymatic hydrolysis of diverse lignocellulose

2019 ◽  
Vol 131 ◽  
pp. 676-681 ◽  
Author(s):  
Huanan Li ◽  
Chaoying Wang ◽  
Wenjing Xiao ◽  
Yuxian Yang ◽  
Pan Hu ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Enzymatic Hydrolysis ◽  
Hydrolysis Of

Enzymatic hydrolysis of soluble starch in a polyethylene glycol-dextran aqueous two-phase system

1990 ◽  
Vol 69 (4) ◽  
pp. 240-243 ◽  
Author(s):  
Kazuhiko Hayashida ◽  
Koji Kunimoto ◽  
Fumihide Shiraishi ◽  
Koei Kawakami ◽  
Yasuhiko Arai
Keyword(s):  
Polyethylene Glycol ◽  
Enzymatic Hydrolysis ◽  
Soluble Starch ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Hydrolysis Of

scholarly journals Characterization of Residual Lignin Obtained by the Enzymatic Hydrolysis of Oil Palm Empty Fruit Bunch Pulps

BioResources ◽  
2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Yin Ying H'ng ◽  
Akiko Nakagawa-Izumi ◽  
Cheu Peng Leh ◽  
Atanu Kumar Das ◽  
Hiroshi Ohi
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Empty Fruit Bunch ◽  
Residual Lignin ◽  
Hydrolysis Of
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