scholarly journals Over-expression of Arabidopsis DnaJ (Hsp40) contributes to NaCl-stress tolerance

2010 ◽  
Vol 9 (7) ◽  
pp. 972-978 ◽  
Author(s):  
Zhichang Zhao ◽  
Wanrong Zhang ◽  
Jinping Yan ◽  
Jianjun Zhang ◽  
Zhen Li Xufeng Liu ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Nacl Stress ◽  
Over Expression

Improvement of outdoor culture efficiency of cyanobacteria by over-expression of stress tolerance genes and its implication as bio-refinery feedstock

2017 ◽  
Vol 244 ◽  
pp. 1294-1303 ◽  
Author(s):  
Hsiang-Yen Su ◽  
Hsiang-Hui Chou ◽  
Te-Jin Chow ◽  
Tse-Min Lee ◽  
Jo-Shu Chang ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Over Expression ◽  
Outdoor Culture

Enhanced NaCl Stress Tolerance in Transgenic Tobacco Expressing Bacterial Choline Dehydrogenase

1996 ◽  
Vol 14 (2) ◽  
pp. 177-180 ◽  
Author(s):  
Gösta Lilius ◽  
Niklas Holmberg ◽  
Leif Bülow
Keyword(s):  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Nacl Stress ◽  
Choline Dehydrogenase

scholarly journals Overexpression of native Musa-miR397 enhances plant biomass without compromising abiotic stress tolerance in banana

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Prashanti Patel ◽  
Karuna Yadav ◽  
Ashish Kumar Srivastava ◽  
Penna Suprasanna ◽  
Thumballi Ramabhatta Ganapathi
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Copper Deficiency ◽  
Plant Biomass ◽  
Abiotic Stress Tolerance ◽  
Nacl Stress ◽  
Marker Genes ◽  
Wild Type ◽  
Trade Offs ◽  
Micro Rnas

Abstract Plant micro RNAs (miRNAs) control growth, development and stress tolerance but are comparatively unexplored in banana, whose cultivation is threatened by abiotic stress and nutrient deficiencies. In this study, a native Musa-miR397 precursor harboring 11 copper-responsive GTAC motifs in its promoter element was identified from banana genome. Musa-miR397 was significantly upregulated (8–10) fold in banana roots and leaves under copper deficiency, correlating with expression of root copper deficiency marker genes such as Musa-COPT and Musa-FRO2. Correspondingly, target laccases were significantly downregulated (>−2 fold), indicating miRNA-mediated silencing for Cu salvaging. No significant expression changes in the miR397-laccase module were observed under iron stress. Musa-miR397 was also significantly upregulated (>2 fold) under ABA, MV and heat treatments but downregulated under NaCl stress, indicating universal stress-responsiveness. Further, Musa-miR397 overexpression in banana significantly increased plant growth by 2–3 fold compared with wild-type but did not compromise tolerance towards Cu deficiency and NaCl stress. RNA-seq of transgenic and wild type plants revealed modulation in expression of 71 genes related to diverse aspects of growth and development, collectively promoting enhanced biomass. Summing up, our results not only portray Musa-miR397 as a candidate for enhancing plant biomass but also highlight it at the crossroads of growth-defense trade-offs.

scholarly journals Ectopic overexpression of abiotic stress-induced rice annexin, OsAnn5 potentiates tolerance to abiotic stresses

2019 ◽  
Author(s):  
Prasanna Boyidi ◽  
Trishla Vikas Shalibhadra ◽  
Halidev Krishna Botta ◽  
Deepanker Yadav ◽  
Pulugurtha Bharadwaja Kirti
Keyword(s):  
Salt Stress ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Peripheral Region ◽  
Upstream Region ◽  
Synthesis Inhibitor ◽  
Post Translational Modifications ◽  
Over Expression ◽  
Onion Epidermal Cells

AbstractThe current study on putative rice annexin OsAnn5 was tried to know its functional role in the abiotic stress tolerance. For this an in silico analysis of its protein sequence and upstream region was carried out. This results in identification of several probable potential sites for post-translational modifications and cis-elements respectively. We have studied the effect of OsAnn5 in the amelioration of abiotic stress tolerance through heterologous expression in transgenic tobacco and E.coli. It is observed that OsAnn5 over expression leads to enhanced tolerance to abiotic stress through efficient scavenging of the ROS and balanced expression of SOD and CAT antioxidant enzymes in both the systems, under stress treatments. Fluorescent signal for transiently expressed EGFP:OsANN5 fusion protein was localized in the peripheral region of the onion epidermal cells under salt stress treatment. Expression analysis of OsAnn5 under ABA synthesis inhibitor, fluridone and salinity stress revealed that OsAnn5 appears to act through an ABA-independent pathway under salt stress and in support to this 35S:OsAnn5 transgenics seedlings exhibited less sensitivity to externally applied ABA.

The continuous accumulation of Na+ in detached leaf sections is associated with over-expression of NTHK1 and salt tolerance in poplar plants

2011 ◽  
Vol 38 (3) ◽  
pp. 236 ◽  
Author(s):  
Ying Zhang ◽  
Ying-Xia Yang ◽  
Xiangming Zhou ◽  
Yan-Hong Jia ◽  
Li-Li Nie ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Nacl Stress ◽  
Controlled Environment ◽  
Wild Type ◽  
Over Expression ◽  
Detached Leaf ◽  
Young Leaves ◽  
Transient Overexpression ◽  
Chlorophyll Retention ◽  
Continuous Accumulation

Detached leaf sections (2 × 2 cm2) from transgenic poplar line 18-1 and its wild type (WT) (Populus × euramericana ‘Neva’) were used to test their salt tolerance and gene expression under controlled environment conditions. The sections from line 18-1 displayed better tolerance to NaCl stress, indicated by high chlorophyll retention and K+ content but low relative electrolyte leakage (REL). Transient overexpression of NTHK1 (Nicotiana tabacum histidine kinase 1) and V-H+-PPase was found in the detached young leaves from line 18-1 after they had been stressed for a few minutes. The activities of vacuolar-type H+-ATPase and H+-PPase in line 18-1 were boosted initially and then decreased to normal level as in unstressed leaves. After sections were stressed for 10 days, the maximal Na+ concentration in line 18-1 was much higher than that in the WT. The higher capacity for Na+ accumulation in line 18-1 may be due to stable Na+ sequestration into the vacuoles. Osmotic stress imposed little effect on REL and chlorophyll content of the sections. The capacity of detached leaf sections in NaCl solution to tolerate stress and to accumulate Na+ may be useful for identifying genotypes with good salt tolerance in poplar and other plants.

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