scholarly journals Effect of combined doses of gamma ray and sodium azide (mutagenic agents) on the morphological traits of some varieties of okra (Abelmoschus esculentus)

2016 ◽  
Vol 11 (32) ◽  
pp. 2968-2973 ◽  
Author(s):  
Hassana Maryam Ati ◽  
Abu Kasimu Adamu
Keyword(s):  
Sodium Azide ◽  
Morphological Traits ◽  
Gamma Ray ◽  
Abelmoschus Esculentus

scholarly journals Morphological mutants of Zoysia japonica Steud. induced by gamma ray irradiation

2019 ◽  
Vol 48 (1) ◽  
pp. 187-194
Author(s):  
Mohd Abdul Halim Bin Baharun Azahar ◽  
Abdul Shukor Juraimi ◽  
Mohd Rafh Yusof ◽  
Abdul Rahim Harun ◽  
SM Samsuzzaman ◽  
...  
Keyword(s):  
Survival Rate ◽  
Morphological Traits ◽  
Leaf Blade ◽  
Gamma Ray ◽  
Mutation Breeding ◽  
Zoysia Japonica ◽  
Single Node ◽  
Morphological Mutants ◽  
Induce Mutagenesis ◽  
Gamma Ray Irradiation

Study of Zoysia japonica morphological improvement and breeding by induced mutation using gamma ray irradiation was conducted. Mutagenesis by gamma ray irradiation was employed using 0, 20, 40, 60, 80, 100, 120 and 140 Gy to treat 30 single node stolons per treatment. It was demonstrated that single node stolon is suitable for gamma ray irradiation mutagenesis in Z. japonica. It has been identified that 76 Gy as the value LD50 is effective to induce mutagenesis on Z. japonica. Survival rate of Z. japonica stolon was greatly reduced when irradiated with higher dosages. This experiment was repeated using LD50 on 1500 single node stolons. Thirty nine morphological mutants were identified and evaluated. Most of the mutants were semi-dwarf and have horizontal growing pattern with reduced internode length and leaf blade length. The altered morphological traits were stable after third cutting back (M1V3) shown by their morphological performance. Mutation breeding is effective in improving Z. japonica when easily recognized cultivars are needed.

scholarly journals Chemical Mutagens Affecting in vitro Behavior of Gardenia jasminoides

2020 ◽  
Vol 30 (2) ◽  
pp. 209-218
Author(s):  
Eman Tawfik ◽  
Mohamed Fathy
Keyword(s):  
Sodium Azide ◽  
Morphological Traits ◽  
Lateral Roots ◽  
Guanidine Hydrochloride ◽  
Leaf Length ◽  
Chemical Mutagens ◽  
Gardenia Jasminoides ◽  
Number Of Leaves ◽  
Number Of Branches

The chemical mutagens, namely sodium azide and guanidine hydrochloride (30, 40 and 50 mM) for each were applied for the in vitro culture of Gardenia jasminoides Ellis. Eight morphological traits were evaluated to calculate the result of different concentrations of mutagens compared to control. In sodium azide, all morphological traits decreased compared to control, except the number of branches and leaves and leaf length, which increases compared to control. Unlike guanidine hydrochloride, all the morphological traits are enhanced in mutants rather than control. The polymorphism percentage resulted from genetic variation varied (57.71% in the case of sodium azide and 67.59% in guanidine hydrochloride). Hence, guanidine hydrochloride is a more powerful mutagen and causes more genetic variations and instability than sodium azide, as was reflected in morphological parameters (i.e., fresh weight, shoot length, root length, number of lateral roots, number of leaves, numbers of branches, leaf length, and leaf width) and physiological traits (i.e., chlorophyll pigmentation and carotenoids). Plant Tissue Cult. & Biotech. 30(2): 209-218, 2020 (December)

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