scholarly journals The influence of protected kapok seed oil supplementation on in vitro ruminal fermentability and linoleic acid status with Etawah crossbred goat rumen fluid and elephant grass as feed

2016 ◽  
Vol 11 (14) ◽  
pp. 1237-1244
Author(s):  
W Widiyanto ◽  
S Surahmanto ◽  
E Kusumanti ◽  
M Mulyono
Keyword(s):  
Linoleic Acid ◽  
Seed Oil ◽  
Rumen Fluid ◽  
Elephant Grass ◽  
Acid Status ◽  
Goat Rumen

In vitro fermentation of browse species using goat rumen fluid in relation to browse polyphenol content and composition

2017 ◽  
Vol 231 ◽  
pp. 1-11 ◽  
Author(s):  
G. Mengistu ◽  
M. Karonen ◽  
J.-P. Salminen ◽  
W.H. Hendriks ◽  
W.F. Pellikaan
Keyword(s):  
Rumen Fluid ◽  
Polyphenol Content ◽  
In Vitro Fermentation ◽  
Browse Species ◽  
Goat Rumen

scholarly journals Comparison of three different rumen fluid as a source of inoculum to evaluate in vitro gas production and digestibility of elephant grass-concentrate mixture

2021 ◽  
Vol 888 (1) ◽  
pp. 012076
Author(s):  
H Soetanto ◽  
RM Aprilia ◽  
MS Pramita ◽  
I Banna
Keyword(s):  
Dry Matter ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Gas Production ◽  
Elephant Grass ◽  
Buffer Solution ◽  
Cattle Breeds ◽  
In Vitro Gas Production ◽  
Concentrate Mixture

Abstract This study aimed at elucidating the use of three different rumen fluid (RF) of indigenous cattle breeds i.e. Bali, Madura and Crossbred Ongole immediately after slaughtered at abattoir to evaluate the nutritive value of elephant grass( EG) -concentrate mixture using a standard in vitro gas production (IVGP) technique. Approximately 500 mg feed dry matter/syringe was added with 50 ml RF-buffer solution and incubated in a 39 0C water bath for 48 hours where gas production was observed at time intervals. Following termination of incubation the content was transferred into tare glass crucible to measure rumen dry matter (RDMD) and organic matter (ROMD) digestibility. The results showed that there was no significant different (P>0.05) in gas production parameters. In contrast, RDMD and ROMD differed significantly (P<0.01) among cattle breeds. RF from OCB resulted in the highest IVGP, RDMD and ROMD as compared with other RF sources. In conclusion, the use of RF from abattoir for IVGP measurement can be warranted using the same source of RF. The highest values resulted from OCB suggests that the abundance and variation in rumen microbiota may exist among cattle breeds.

An in vitro cultured rumen inoculum improves nitrogen digestion in mulga-fed sheep

1997 ◽  
Vol 48 (4) ◽  
pp. 403 ◽  
Author(s):  
S. M. Miller ◽  
A. V. Klieve ◽  
J. J. Plumb ◽  
R. Aisthorpe ◽  
L. L. Blackall
Keyword(s):  
Rumen Fluid ◽  
Mixed Cultures ◽  
Microbial Composition ◽  
Widespread Adoption ◽  
The Difference ◽  
Micro Organisms ◽  
Laboratory Fermentor ◽  
Feral Goat ◽  
Goat Rumen

Mixed cultures of anaerobic micro-organisms were derived from feral goat rumen fluid (FGRF) using a laboratory fermentor to selectively culture microbes actively degrading mulga, and were evaluated as rumen inocula in digestion and liveweight studies with mulga-fed sheep. When placed in the rumen of sheep, FGRF enhances mulga digestion; however, limited supplies of feral goats, the labour involved in locating and mustering goats, and likely variations in the microbial composition of FGRF between animals and localities make the production of an in vitro cultured inoculum a desirable alternative to enable widespread adoption. The cultured inoculum significantly (P < 0·05) improved nitrogen digestion and retention in mulga-fed sheep by 16 and 76%, respectively. Inocula consisting of simplified mixtures of bacteria isolated from sheep, feral goats, and native marsupials did not affect mulga digestion. In the first of 2 liveweight studies, sheep inoculated with the fermentor inoculum lost significantly less weight than uninoculated sheep for the first 57 days (0·3 v. 4·6 kg); however, after 83 days the difference in the rate of liveweight loss between the fermentor inoculum group and the uninoculated sheep was not significant (53 v. 95 g/day). In the second study, liveweight loss was not significantly reduced by the fermentor inoculum. An inoculum based on FGRF, and produced in vitro using a fermentor, is potentially valuable to grazing enterprises reliant on mulga-fed sheep. However, problems in generating a consistent inoculum need to be addressed before such an inoculum can be generally considered.

scholarly journals Influence of Different Vegetable Oils on In Vitro Ruminal Fermentability and Nutrient Digestibility in Ettawah Crossbred Goat

2020 ◽  
Vol 21 (1) ◽  
pp. 22
Author(s):  
Anis Muktiani ◽  
Nurul Arifah ◽  
Widiyanto Widiyanto
Keyword(s):  
Vegetable Oil ◽  
Palm Oil ◽  
Seed Oil ◽  
Corn Oil ◽  
Rumen Fluid ◽  
Block Design ◽  
Nutrient Digestibility ◽  
Dry Matter Digestibility ◽  
Randomized Block Design

This research was aimed to determine the effect of supplementation of vegetable oil (corn oil, palm oil and kapok seed oil) on in vitro ruminal fermentability and nutrient digestibility. Experiment design used was Randomized Block Design with four treatments and four replication based on rumen fluid sampling time. The four treatments namely R0 = feed without oil supplementation; R1 = R0 + 5% corn oil; R2 = R0 + 5% palm oil; and R3 = R0 + 5% kapok seed oil. The result showed that supplementation of 5% vegetable oil did not affect (P>0,05) the rumen pH, A/P ratio, and efficiency of energy conversion. The total VFA, acetate, propionate, butyrate, methane and NH3 was higher (P<0,05) in supplementation of 5% vegetable oil than he control. The population of protozoa, microbial protein, in vitro dry matter digestibility (IVDMD), organic matter digestibility (IVOMD), and crude fiber digestibility (IVCFD) was lower (P<0,05) in supplementation of 5% compared to the control. Population of protozoa decreased until 58,76% for R1; 66,89% for R2; and 43,33% for R3. It can be concluded that supplementation of 5% vegetable oil decreased the population of protozoa, increased the production of VFA and NH3. Supplementation of 5% kapok seed oil resulting the highest of total VFA, acetate, propionate, butyrate and NH3 among other treatments.

Development of Protocol for Lyophilization of Goat Rumen Fluid

2021 ◽  
Author(s):  
A. Ruba Nanthini ◽  
C. Valli ◽  
L. Radhakrishnan ◽  
D. Balasubramanyam ◽  
A. Mangalagowri
Keyword(s):  
Rumen Fluid ◽  
Milk Powder ◽  
Skim Milk ◽  
Skim Milk Powder ◽  
Gas Production ◽  
Time Duration ◽  
Rumen Microbes ◽  
Alternate Source ◽  
Goat Rumen

Background: Rumen fluid has been used as microbial inoculum to treat indigestion in ruminant animals and to conduct in vitro rumen fermentation experiments. Lyophilization of the goat rumen fluid will provide continuous supply of rumen inoculums either for laboratory studies or for transfaunation in treating digestive disorders sequelae to high grain rations. However, no standard protocol is available for lyophilizing goat rumen fluid. Hence, this study was designed to develop a protocol to lyophilize goat rumen fluid as an alternate source for fresh goat rumen fluid. Methods: The study was conducted using 5 × 3 × 3 factorial design with four different cryoprotectants viz., 10% skim milk powder, 10% skim milk powder + 5% sodium glutamate, 5% glycerol, 5% DMSO and no cryoprotectant, at three pre freezing (2, 24 and 48 hours) and three freeze drying (8, 24 and 32 hours) time intervals to standardize protocol for lyophilizing goat rumen fluid. The viability of rumen microbes in the “lyophilized goat ruminal inoculum”, was determined via in vitro gas production study. Result: Pre freezing (-80°C deep freezer) duration of 48 hours with 32 hours of time duration in lyophilizer (-45°C) was ideal for lyophilizing goat rumen fluid with or without the addition of various cryoprotectants. Glycerol used at 5% as cryoprotectant resulted in significantly (P less than 0.05) highest gas production at all (12, 24 and 48) incubation hours studied indicating better viability of rumen microbes.

scholarly journals Influence of inoculated maize silage and sunflower oil on the in vitro fermentation, ciliate population and fatty acid outputs in the rumen fluid collected from sheep

2010 ◽  
Vol 55 (No. 3) ◽  
pp. 105-115 ◽  
Author(s):  
Z. Váradyová ◽  
S. Kišidayová ◽  
A. Lauková ◽  
D. Jalč
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Sunflower Oil ◽  
Rumen Fluid ◽  
Gas Production ◽  
Time Interval ◽  
Short Time Interval ◽  
Maize Silage ◽  
Ciliate Species

The effect of maize silage (MS) supplemented with sunflower oil (SO) on the rumen fermentation parameters, growth of ciliate population and fatty acid outputs<I> </I>was investigated for 72 hours using a batch culture fermentation technique. The rumen fluid from ruminally fistulated sheep was mixed with McDougall’s buffer (1:1) and added (35 ml) to fermentation bottles containing 1.5 g (0.38 g of DM) of MS with or without SO (30 g/kg of DM). Four types of MS were used: uninoculated (MS) or inoculated with <I>Lactobacillus plantarum</I> CCM 4000 (MS+LP), <I>Lactobacillus fermentum</I> LF2 (MS+LF) or <I>Enterococcus faecium </I>CCM 4231 (MS+EF) in simultaneous incubations at 39 ± 0.5°C for 72 h <I>in vitro</I>. Total gas production was decreased by SO (by 16–17%) in MS and all inoculated MS. Methane production was not significantly influenced by SO. The concentration of total volatile fatty acids, molar proportions of acetate, propionate and <I>n</I>-butyrate were influenced by SO (<I>P&nbsp;&lt;&nbsp;</I>0.05; <I>P &lt; </I>0.01; <I>P &lt; </I>0.001). SO had no significant effect on the total ciliate number and growth of the examined ciliate species <I>Entodinium </I>spp., <I>Dasytricha ruminantium</I>, <I>Polyplastron multivesiculatum</I>,<I> Enoploplastron triloricatum</I> and<I> Diplodinium denticulatum</I>. The number of <I>Dasytricha ruminantium</I> with MS+LP was higher (<I>P &lt; </I>0.01) as compared to MS. Outputs of <I>trans</I> vaccenic acid (TVA), linoleic acid, conjugated linoleic acid (CLA) and α-linolenic acid were influenced by SO (<I>P&nbsp;&lt;&nbsp;</I>0.05;<I> P &lt; </I>0.01; <I>P &lt; </I>0.001). However, the output of CLA was increased only in MS+LF (<I>P &lt;</I> 0.001). It can be concluded that the supplementation of sunflower oil into maize silage is not effective as dietary antiprotozoal agents in a short-time interval, but it might positively affect the rumen bacterial population and activities. Sunflower oil with inoculated and uninoculated maize silage could be effective for an increase of TVA in the rumen fluid of sheep.&nbsp; &nbsp;

scholarly journals Dietary treatment with omega fatty acids mediates in vitro rumen fermentation kinetics and reduce methane emission in water buffalo

2021 ◽  
Vol 20 (9) ◽  
pp. 1801-1809
Author(s):  
Mengwei Li ◽  
Faiz-ul Hassan ◽  
Lijuan Peng ◽  
Hossam Mahrous Ebeid ◽  
Zhenhua Tang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Linolenic Acid ◽  
Rumen Fluid ◽  
Rumen Fermentation ◽  
Response To Treatment ◽  
Microbial Populations ◽  
Omega Fatty Acids ◽  
In Vitro Rumen Fermentation

Purpose: To investigate the effect of dietary supplementation of two omega fatty acids on in vitro rumen  fermentation, microbial populations, total gas and methane (CH4) production．Methods: Both linoleic and linolenic acids were supplemented at 0 (control), 1, 3, 5 and 7 % of dry matter (DM) in a ration with a high roughage to concentrate ratio (70: 30). Total gas and CH4  were measured at 3, 6, 9, 12 and 24 h of fermentation while pH, volatile fatty acids (VFA), and ammonia nitrogen (NH3-N) concentrations were measured at 24 h using buffalo rumen fluid in an in vitro batch culture system. Microbial populations were determined using 16S-rDNA gene primers by RT-PCR.Results: The results revealed that linoleic acid at 3, 5 and 7 % decreased the concentration of NH3-N (p< 0.05) but linolenic acid at 5 and 7 % increased NH3-N (p < 0.05). A linear decrease (p <0.001) in acetate and butyrate, coupled with linear increase (p <0.001) in propionate was observed in response to treatment. Furthermore, supplementation of 3, 5 and 7 % of both fatty acids linearly (p < 0.001) decreased total gas and CH4 production when compared to the control. The addition of linoleic acid linearly (p < 0.001) decreased the number of protozoa without affecting methanogens, while linolenic acid linearly and quadratically (p < 0.001) reduced the population of both protozoa and methanogens (p < 0.05).Conclusion: Linolenic acid is more effective at a 3 % level in reducing methane production (up to 63 %) in high roughage diets.

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