scholarly journals Effects of genotypes and medium on callus induction of proso millet derived from anther culture

2012 ◽  
Vol 7 (43) ◽  
pp. 5766-5771
Author(s):  
Wu Limin ◽  
Yang Tianyu ◽  
Dong Guojun ◽  
He Jihong ◽  
Dong Kongjun ◽  
...  
Keyword(s):  
Anther Culture ◽  
Callus Induction ◽  
Proso Millet

Inheritance of callus induction ability in rice anther culture

1997 ◽  
Vol 42 (14) ◽  
pp. 1209-1211
Author(s):  
Ping He ◽  
Ying Chen ◽  
Lishuang Shen ◽  
Chaofu Lu ◽  
Lihuang Zhu
Keyword(s):  
Anther Culture ◽  
Callus Induction

scholarly journals In vitro shoot regeneration through anther culture of Brassica spp.

1970 ◽  
Vol 35 (2) ◽  
pp. 331-341 ◽  
Author(s):  
MA Sayem ◽  
M Maniruzzaman ◽  
SS Siddique ◽  
M Al-Amin
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Shoot Regeneration ◽  
Callus Induction ◽  
Maximum Rate ◽  
Media Composition ◽  
Shoot Initiation ◽  
The Media

The experiment was conducted to investigate the performance of three different genotypes (BARI Sarisha-6, BARI Sarisha-8, and BARI Sarisha-11) in two different media viz., MS and B5 with different concentrations of phytohormone (2, 4-D) for callus induction from uninucleate stage anthers of Brassica and subsequent plant regeneration in MS media with different concentrations of phytohormone (BAP and NAA). Among the genotypes, BARI Sarisha-8 showed the best performance for all the parameters of callus induction. The performance of BARI Sarisha-6 was poor compared to others. Maximum rate of callus induction (%) was observed in MS + 0.5 mg/L 2, 4-D followed by B5 + 0.5 mg/L 2,4-D. The media combination MS + 1.0 mg/L BAP 0.3 mg/L 2,4-D showed the best performance for maintenance of calli. Significant variations were observed among the genotypes and media composition for shoot regeneration. Among the genotypes, BARI Sarisha-8 showed the best performance for shoot regeneration followed by BARJ Sarisha-l1. The genotype BARI Sarisha-8 produced higher percent of shoots/calli and required minimum days for shoot initiation. Higher percent calli without shoot were produced by the genotype BARI Sarisha-6. The media combination MS + 2.0 mg/L BAP + 0.5 mg/L NAA showed the best performance for shoot regeneration and required maximum days for shoot initiation. Keywords: Regeneration; BARI Sarisha-6; BARI Sarisha-8; BARI Sarisha-11; anther culture; phytohormone  DOI: 10.3329/bjar.v35i2.5896Bangladesh J. Agril. Res. 35(2) : 331-341, June 2010

scholarly journals Improvement of Selected Induction Culture Media on Callus Induction in Anther Culture of Anthurium and a Histological Study on its Callus Formation

2012 ◽  
Vol 12 (2) ◽  
pp. 93 ◽  
Author(s):  
Budi Winarto ◽  
Nurhayati Ansori Mattjik ◽  
Agus Purwito ◽  
Budi Marwoto
Keyword(s):  
Anther Culture ◽  
Callus Induction ◽  
Culture Media ◽  
Callus Formation ◽  
Histological Study ◽  
Block Design ◽  
Middle Layer ◽  
Shoot Formation ◽  
Anther Wall ◽  
Randomized Complete Block Design

Improvement of selected induction culture media on callus induction in anther culture of anthurium and a histologicalstudy on its callus formation were studied at the tissue culture laboratory of the Indonesian Ornamental CropsResearch Institute from February to October 2008. The objectives of the study were to optimize selected media forcallus formation, reveal cell origin of callus derived from anther culture and shoot formation process. Selectedmedia improved in the study were 1) MMS-TBN containing 0,5 mg/l TDZ, 1,0 mg/l BAP and 0,01 mg/l NAA (Winartomedium, WM) and 2) MMS III supplemented with 1,5 mg/l TDZ, 0,75 mg/l BAP and 0,02 mg/l NAA (Winarto andRachmawati medium, WRM). Improvement treatments were carried out by omission and application of 2,4-D in 0.5mg/l and reduction of medium strength of full, half, quarter, one eighth, one sixteenth, and zero strength. Afactorial experiment was arranged using a randomized complete block design with four replications. Results ofthis study indicated that the highest callus induction was clearly established in WRM. The medium stimulatedpotential growth of anther (PGA) up to 81% with 49% of percentage of anther regeneration (PAR) and 2.7 number ofcallus formed per replication (NCF). Significant improvement in callus formation was also recorded by reduction ofmedium strength of WRM to one eighth compared to others. The reduction induced PGA up to 58% with 29% of PARand 1.8 NCF. From histological studies it was well recognized that regenerated callus on half anthers cultured wasoriginated from middle layer cells of anther wall. The morphogenic response of anther wall cells caused primarilyon no androgenesis effect in microspore cells.

scholarly journals Regenerasi dan Aklimatisasi Kultur Antera Enam Persilangan F1 Padi Sawah

2016 ◽  
Vol 44 (2) ◽  
pp. 133
Author(s):  
Cucu Gunarsih ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Dan Muhamad Syukur
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Callus Induction ◽  
Rainfed Rice ◽  
Randomized Design ◽  
Doubled Haploid Plants ◽  
Completely Randomized Design ◽  
N6 Medium ◽  
Haploid Plants

ABSTRACT<br /><br />The breeding of rainfed rice tolerant to drought can be accomplished using anther culture. The objectives of this research were to determine regeneration abilities of six F1 anther culture and its acclimatization ability. The experiment was arranged in completely randomized design with 14 replications. The treatments consisted of six F1 derived from crossing:  INPARI 18 x IR83140-B-11-B (G1), INPARI 18 x B12825E-TB-1-25 (G2), INPARI 18 x IR87705-14-11-B-SKI-12 (G3), INPARI 22 x IR83140-B-11-B (G4), Bio-R81 x O18b-1 (G5), Bio-R82-2 x O18b-1 (G6). Media for callus induction was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1 kinetin + 1.0 mM putresin + 60 g L-1 sucrosa, media for regeneration was based on MS + 0.5 mg L-1 NAA + 2.0 mg L-1 kinetin + 1.0 mM  putresin, and media for rooting was based on  MS + 0.5 mg L-1 IBA + 30 g L-1 sucrosa. The result indicated that all six F1 had different ability in anther culture. Bio-R82-2 x O18-b1 (G6) and  Bio-R81 x O18-b1 (G5) F1 genotype had good response both of callus induction and plant regeneration. These two F1 genotypes also gave the highest ratio of green planlet production to number of anther inoculated (GP:AI) were 5.50% and 4.65%,  respectively. In this research, there were identified doubled haploid plants were developed from 4 F1 derived cross namely G2 (2 plants), G3 (4 plants),  G5 (21 plants), and G6 (26 plants).<br /><br />Keywords: Callus induction, doubled haploid, rice<br /><br />

Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration in Anther Culture of Rice

2004 ◽  
Vol 7 (3) ◽  
pp. 331-334 ◽  
Author(s):  
Md. Monirul Islam ◽  
Sanjay Kumar Adhikar . ◽  
Purnendu Gain . ◽  
Md. Mizanur Rahman . ◽  
Noor-e-Alam Siddique .
Keyword(s):  
Plant Regeneration ◽  
Plant Growth ◽  
Anther Culture ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction

scholarly journals Haploid plantlet regeneration through anther culture in oilseed Brassica species

1970 ◽  
Vol 34 (4) ◽  
pp. 693-703 ◽  
Author(s):  
MA Alam ◽  
MA Haque ◽  
MR Hossain ◽  
SC Sarker ◽  
R Afroz
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Growth Regulators ◽  
Callus Induction ◽  
Brassica Species ◽  
Plantlet Regeneration ◽  
Root Induction ◽  
Ms Medium ◽  
Half Strength

Anther of five varieties of Brassica species, namely BARI Shariaha-7, Tori-7, Agrani, Daulat and Safal were cultured in vitro to observe their regeneration potentiality. Different concentrations and combinations of growth regulators were supplemented in MS medium. The range of callus induction was 12.50-87.50 %. Maximum callus induction (75.00%) was observed on MS +4 mg/L 2, 4-D + 1.0 mg/L BAP. Among the genotypes, BARI Sharisha-7 showed the highest percentage of callus induction (60.42%). Among the treatments, highest percentage of shoot regeneration (75.00%) was observed on MS + 4 mg/L BAP + 1.0 mg/L NAA. BARI Sharisha-7 also showed the highest rate of plant regeneration (66.67%). Root induction was highest (75%) on half strength MS medium supplemented with 1.0 mg/L IBA and 0.5 mg/L NAA. The plantlets with sufficient roots thus obtained were transferred successfully to plastic pots and subsequently to the field. BARI Sharisha-7 and Tori-7 survived easily in the pots as well as in the field but Safal was very poor in survivability both in the pots and in the field. Key Words: Brassica; haploid; anther culture; in vitro regeneration.DOI: 10.3329/bjar.v34i4.5844Bangladesh J. Agril. Res. 34(4) : 693-703, December 2009 

scholarly journals Pembentukan Genotipe Padi Berumur Sangat Genjah melalui Kultur Antera

2016 ◽  
Vol 18 (2) ◽  
pp. 54
Author(s):  
Iswari S. Dewi ◽  
A. Dinar Ambarwati ◽  
Aniversari Apriana ◽  
Atmitri Sisharmini ◽  
Ida H. Somantri ◽  
...  
Keyword(s):  
Anther Culture ◽  
Callus Induction ◽  
Culture Media ◽  
Oryza Sativa L ◽  
Doubled Haploids ◽  
Heading Date ◽  
Population Increase ◽  
Early Maturity ◽  
Early Maturing ◽  
Pure Lines

<p>Development of Very Early Maturing Rice Genotypes through Anther Culture. Iswari S. Dewi, A. Dinar Ambarwati, Aniversari Apriana, Atmitri Sisharmini, Ida H. Somantri, Bambang Suprihatno, and Iman Ridwan. Rice is the most important food crop in Indonesia. Increase in production is needed due to population increase. Rice production in rainfed area is contributed the second after irrigated area. Rainfed condition requiring very early maturity (90-104 days) varieties. Rice anther culture can be applied to accelerate obtainment of doubled haploids (DHs) or pure lines needed in rice breeding. The experiment was aimed to obtain pure lines for developing very early maturing and high yielding rice varieties. Materials used for anther culture were F1s of Fatmawati/Kinamase, Inpari 1/Kinamase, Fatmawati/ Waseaikoku, Inpari 1/Waseaikoku, Fatmawati/IR71146, Inpari 1/IR71146, OM4495/Silugonggo, IR7146/Dodokan, and IR71730/OM1490. Anther culture media were N6 + NAA 2,0 mg/l + kinetin 0,5 mg/l for callus induction, MS+ NAA 0,5 mg/l + kinetin 2,0 mg/l for plantlet regeneration, and MS + 0,5 mg/l IBA for rooting. Putrescine 10-3 M was added to callus induction and regeneration media. The results shown that calli forming green plantlet (CFGP) were ranged from 0.25 to 83.33%. Fatmawati/Kinamase gave the highest CFGP (245 calli), followed by Inpari 1/Kinamase (78 calli) and Fatmawati/ Waseaikoku (68 calli). Total green plantlets obtained were 2.038 plantlets. After plantlet acclimatization and greenhouse grow-out, we obtained 507 DHs. The evaluation of 100 DHs at farmer field (Ciranjang District in Cianjur), based on their 50% heading date of 65 days, resulted in 33 lines cathegorized as very early maturing lines (+100 days). They were 18 lines from Fatmawati/Kinamase, 5 lines from Inpari 1/Kinamase, 8 lines from Fatmawati/Waseaikoku, and 2 lines from Inpari 1/ Waseaikoku.</p><p> </p><p><strong>Abstrak</strong></p><p>Padi (Oryza sativa L.) merupakan komoditi pangan terpenting di Indonesia. Peningkatan produksi diperlukan seiring dengan peningkatan jumlah penduduk. Lahan sawah tadah hujan merupakan lumbung padi kedua setelah sawah irigasi. Kondisi lahan sawah tadah hujan memerlukan varietas-varietas padi berumur sangat genjah (90-104 hari). Teknik kultur antera dapat digunakan untuk mempercepat perolehan tanaman dihaploid (DH) atau galur murni dalam pemuliaan padi. Penelitian ini bertujuan untuk mendapatkan galur-galur murni yang akan digunakan dalam perakitan padi berdaya hasil tinggi dan berumur sangat genjah. Bahan tanaman yang digunakan untuk kultur antera adalah malai dari tanaman F1 hasil persilangan Fatmawati/Kinamase, Inpari 1/Kinamase, Fatmawati/Waseaikoku, Inpari 1/Waseaikoku, Fatmawati/IR71146, Inpari 1/ IR71146, OM4495/Silugonggo, IR7146/Dodokan, dan IR71730/OM1490. Media kultur antera adalah N6 + NAA 2,0 mg/l + kinetin 0,5 mg/l untuk media induksi kalus, MS+ NAA 0,5 mg/l + kinetin 2,0 mg/l untuk media regenerasi, dan MS + 0,5 mg/l IBA untuk media perakaran. Putresine 10-3 M ditambahkan pada media induksi kalus dan regenerasi. Hasil penelitian menunjukkan bahwa kalus yang menghasilkan tanaman hijau (KMTH) berkisar antara 0,25-83,33%. Persilangan Fatmawati/ Kinamase memberikan KMTH tertinggi (245 kalus), diikuti oleh Inpari 1/Kinamase (78 kalus) dan Fatmawati/ Waseaikoku (68 kalus). Total tanaman hijau yang diperoleh adalah 2.038 planlet dihaploid, namun diperoleh 507 tanaman setelah planlet diaklimatisasi dan tanaman ditumbuhkan di rumah kaca. Evaluasi terhadap 100 DH dilakukan di lahan petani Ciranjang, Cianjur. Berdasarkan hari berbunga 50% (65 hari setelah semai), diperoleh 33 galur yang termasuk kategori sangat genjah (dipanen +100 hari). Galur-galur tersebut adalah 18 galur dari persilangan Fatmawati/Kinamase, 5 galur dari persilangan Inpari 1/Kinamase, 8 galur dari persilangan Fatmawati/ Waseaikoku, dan 2 galur dari persilangan Inpari 1/ Waseaikoku.</p>

scholarly journals Pengaruh Glutamin dan Serin terhadap Kultur Anter Anthurium andraeanum cv. Tropical

2011 ◽  
Vol 21 (4) ◽  
pp. 293 ◽  
Author(s):  
Budi Winarto
Keyword(s):  
Anther Culture ◽  
Callus Induction ◽  
Culture Medium ◽  
Callus Formation ◽  
Potential Growth ◽  
Randomized Design ◽  
Ornamental Crops ◽  
Completely Randomized Design ◽  
Anthurium Andraeanum ◽  
Positive Effect

Kultur anter merupakan salah satu teknologi haploid penting dalam produksi tanaman haploid ganda dan berhasil diaplikasikan pada berbagai jenis tanaman, namun aplikasi pada Anthurium belum pernah dilaporkan. Penelitian dan pengembangan kultur anter Anthurium yang difokuskan untuk mempelajari pengaruh glutamin dan serin terhadap induksi, pertumbuhan, dan regenerasi kalus dilakukan di Laboratorium Kultur Jaringan Balai Penelitian Tanaman Hias dari bulan Januari sampai dengan September 2008. Tujuan penelitian ialah mengetahui pengaruh kombinasi konsentrasi glutamin dan serin terhadap induksi, pertumbuhan, dan regenerasi kalus pada kultur anter Anthurium. Spadik Anthurium andraeanum cv. Tropical, kalus hasil kultur anter serta medium Winarto dan Teixeira digunakan dalam studi ini. Glutamin dan serin pada konsentrasi 0, 250, 500, dan 750 mg/l diuji dalam percobaan ini. Percobaan disusun menggunakan rancangan acak lengkap pola faktorial dengan empat ulangan. Hasil studi menunjukkan bahwa penambahan glutamin dan serin pada medium terseleksi belum memberikan pengaruh yang signifikan terhadap induksi, pertumbuhan, dan regenerasi kalus. Glutamin pada konsentrasi 250 mg/l menginduksi potensi tumbuh anter hingga 48% dengan 21% anter beregenerasi dan 1,3 anter per perlakuan membentuk kalus. Sementara serin pada 500 mg/l merupakan konsentrasi yang paling potensial dalam induksi kalus dengan 55% potensi tumbuh anter, 24% anter beregenerasi, dan 1,4 anter per perlakuan membentuk kalus. Glutamin 250 mg/l merupakan konsentrasi terbaik dibanding konsentrasi yang lain dalam mendukung pertumbuhan dan regenerasi kalus. Perlakuan tersebut tanpa serin mampu menginduksi potensi pertumbuhan kalus hingga 77% dengan volume kalus mencapai 237 mm3 dan empat tunas dihasilkan per eksplan. Sementara perlakuan serin justru mereduksi pertumbuhan dan regenerasi kalus dan menstimulasi senesensi kalus yang berdampak pada pencoklatan dan kematiannya. Dari hasil penelitian ini dapat disarankan penggunaan glutamin dibanding serin dalam meningkatkan keberhasilan kultur anter Anthurium.<br /><br /><br /><br />Anther culture is one of important haploid technologies in producing double haploid lines and successfully applied in many plants, while the application in Anthurium is not reported yet. Research and development in anther culture of Anthurium focusing on studying the effect of glutamine and serine on callus induction, growth, and its regeneration was conducted at Tissue Culture Laboratory of Indonesian Ornamental Crops Research Institute from January untill September 2008. Objective of this study was to know the effect of glutamine and serine on callus induction, growth, and its regeneration in anther culture of Anthurium. Spadix of Anthurium andraeanum cv. Tropical, callus derived from anther and Winarto and Teixeira medium were utilized in the study. Glutamine and serine of 0, 250, 500, and 750 mg/l were tested in the experiments. Factorial experiment was arranged by completely randomized design with four replications. Results of the study indicate that addition of glutamine and serine in selected culture medium gave moderate significant effect on induction, growth, and regeneration of callus. Glutamine in 250 mg/l induced potential growth of anther up to 48% with 21% regenerated anthers and 1.3 anthers per treatment producing calli, while 500 mg/l of serine was better concentration in callus formation with 55% potential growth of callus, 24% regenerated anthers and 1.4 anthers per treatment producing calli. In growth and regeneration of callus, supplementation of serine reduced callus capacity in growth and production of shoots and stimulated callus senescence causing browning and death of it, while 250 mg/l glutamine exhibited positive effect on them. The treatment without serine was able to induce potential growth of callus up to 77% with 237 mm3 per callus and four shoots produced per explants. Results of the study suggest application of glutamine rather than serine in improving anther culture of Anthurium.<br /><br />

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