scholarly journals Inhibitory effect of extract from chewing gum containing mutastein on insoluble glucan synthesis and cellular adherence to glass wall of Streptococcus mutans.

1987 ◽  
Vol 37 (1) ◽  
pp. 21-29
Author(s):  
Yasunori NAKAMURA ◽  
Haruhiro KUWASHIMA ◽  
Hiroyoshi IWASAKI ◽  
Yoshio ITOH ◽  
Taizo MASUHARA
Keyword(s):  
Streptococcus Mutans ◽  
Inhibitory Effect ◽  
Chewing Gum ◽  
Glass Wall ◽  
Insoluble Glucan ◽  
Glucan Synthesis

scholarly journals Streptococcus mutans Dextransucrase: Functioning of Primer Dextran and Endogenous Dextranase in Water-Soluble and Water-Insoluble Glucan Synthesis

1977 ◽  
Vol 16 (2) ◽  
pp. 637-648 ◽  
Author(s):  
Greg R. Germaine ◽  
Susan K. Harlander ◽  
Woon-Lam S. Leung ◽  
Charles F. Schachtele
Keyword(s):  
Streptococcus Mutans ◽  
Water Soluble ◽  
Insoluble Glucan ◽  
Glucan Synthesis

scholarly journals Cationic Lipid Content in Liposome-Encapsulated Nisin Improves Sustainable Bactericidal Activity against Streptococcus mutans

2016 ◽  
Vol 10 (1) ◽  
pp. 360-366 ◽  
Author(s):  
Kazuo Yamakami ◽  
Hideaki Tsumori ◽  
Yoshitaka Shimizu ◽  
Yutaka Sakurai ◽  
Kohei Nagatoshi ◽  
...  
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Cationic Lipid ◽  
Cationic Liposome ◽  
Bactericidal Effect ◽  
Insoluble Glucan ◽  
Inhibitory Efficiency ◽  
Bactericidal Activities ◽  
Glucan Synthesis ◽  
Sustained Inhibition

An oral infectious disease, dental caries, is caused by the cariogenic streptococci Streptococcus mutans. The expected preventive efficiency for prophylactics against dental caries is not yet completely observed. Nisin, a bacteriocin, has been demonstrated to be microbicidal against S. mutans, and liposome-encapsulated nisin improves preventive features that may be exploited for human oral health. Here we examined the bactericidal effect of charged lipids on nisin-loaded liposomes against S. mutans and inhibitory efficiency for insoluble glucan synthesis by the streptococci for prevention of dental caries. Cationic liposome, nisin-loaded dipalmitoylphosphatidylcholine/phytosphingosine, exhibited higher bactericidal activities than those of electroneutral liposome and anionic liposome. Bactericidal efficiency of the cationic liposome revealed that the vesicles exhibited sustained inhibition of glucan synthesis and the lowest rate of release of nisin from the vesicles. The optimizing ability of cationic liposome-encapsulated nisin that exploit the sustained preventive features of an anti-streptococcal strategy may improve prevention of dental caries.

scholarly journals Recombination between gtfB andgtfC Is Required for Survival of a dTDP-Rhamnose Synthesis-Deficient Mutant of Streptococcus mutans in the Presence of Sucrose

1999 ◽  
Vol 67 (7) ◽  
pp. 3693-3697 ◽  
Author(s):  
Yoshihisa Yamashita ◽  
Kiyotaka Tomihisa ◽  
Yoshio Nakano ◽  
Yoshihiro Shimazaki ◽  
Takahiko Oho ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Fusion Gene ◽  
Survival Rates ◽  
Synthetic Activity ◽  
Gene Fusions ◽  
Extracellular Water ◽  
Insoluble Glucan ◽  
Glucan Synthesis ◽  
Glass Surfaces ◽  
Reduced Water

ABSTRACT The rml genes are involved in dTDP-rhamnose synthesis in Streptococcus mutans. A gene fusion betweengtfB and gtfC, which both encode extracellular water-insoluble glucan-synthesizing enzymes, accompanied by inactivation of the rml genes was observed for cells grown in the presence of sucrose. The survival rates of rmlmutants isolated in the absence of sucrose were drastically reduced in the presence of sucrose. The rates were consistent with the frequency of spontaneous gene fusions between gtfB andgtfC, suggesting that the spontaneous recombinant organisms were selected in the presence of sucrose. The rml mutants with a gtfB-gtfC fusion gene had markedly reduced water-insoluble glucan synthetic activity and lost the ability to colonize glass surfaces in the presence of sucrose. These results suggest that the rml mutants of S. mutans, which are defective in dTDP-rhamnose synthesis, can survive only in the absence of water-insoluble glucan synthesis.

scholarly journals Inhibitory Potential of Mangiferin on Glucansucrase Producing Streptococcus mutans Biofilm in Dental Plaque

2020 ◽  
Vol 10 (22) ◽  
pp. 8297
Author(s):  
Promise M. Emeka ◽  
Lorina I. Badger-Emeka ◽  
Hairul-Islam M. Ibrahim ◽  
Krishnaraj Thirugnanasambantham ◽  
Jamal Hussen
Keyword(s):  
Streptococcus Mutans ◽  
Virulence Genes ◽  
Inhibitory Effect ◽  
Water Soluble ◽  
Oral Microbiota ◽  
Sem Images ◽  
Bacteria Growth ◽  
Dental Biofilm ◽  
Reverse Transcription Pcr ◽  
Glucan Synthesis

Glucansucrase secreted by Streptococcus mutans and composed of virulence genes alters oral microbiota, creating adherent environment for structural bacteria colony forming dental biofilm. The present investigation studied the inhibitory and binding potentials of mangiferin against S. mutans and its enzyme glucansucrase implicated in biofilm formation. Antibacterial activity against planktonic S. mutans was carried out. Using reverse transcription PCR, the expression of crucial virulence genes, gtfB, gtfC, gtfD, gbpB, and comDE were determined. The effect of mangiferin on teeth surfaces biofilm was ascertained by scanning electron microscopy (SEM). Docking analysis of S. mutans glucansucrase and mangiferin revealed the binding energy of −7.35 and ten hydrogen interactions. Antibacterial study revealed that mangiferin was not lethal to planktonic S. mutans, but a concentration-dependent inhibition of glucansucrase activity was observed. The inhibitory effect of water-insoluble glucan synthesis was apparently more marked relative to water-soluble glucan synthesis attenuation. Mangiferin significantly downregulated the expression of the virulence genes, indicating a mechanism involving glucanotranferases, specifically inhibiting colony formation by attenuating bacterial adherence. SEM images revealed that S. mutans biofilm density was scanty in mangiferin treated teeth compared to non-treated control teeth. Our data therefore suggest that mangiferin inhibited S. mutans biofilms formation by attenuating glucansucrase activities without affecting bacteria growth.

Sign in / Sign up

Export Citation Format

Share Document