The Effectiveness and Tolerability of Preformed Growth Factors Vehiculated Through Iontophoresis on Patients with Androgenetic Alopecia and Telogen Effluvium: A Clinical Study

Dermatology Practical & Conceptual ◽

10.5826/dpc.1103a82 ◽

2021 ◽

pp. 2021082

Author(s):

Aurora Maria Alessandrini ◽

Francesca Bruni ◽

Bianca Maria Piraccini ◽

Michela Starace

Keyword(s):

Growth Factors ◽

Combined Therapy ◽

Hair Shaft ◽

Hair Follicles ◽

Androgenetic Alopecia ◽

Treatment Modalities ◽

Self Assessment ◽

Hair Density ◽

Pattern Distribution ◽

Assessment Questionnaire

Background: Androgenetic alopecia is characterized by a progressive miniaturization of hair follicles in a pattern distribution in genetically predisposed individuals. The efficacy of conventional therapies is variable, therefore there is a need for adjuvant and newer treatment modalities to provide faster and better outcomes. Objectives: Evaluation of the efficacy and tolerability of a combined therapy: preformed growth factors vehiculated through iontophoresis in patients with androgenetic alopecia and associated telogen effluvium, to obtain faster hair regrowth. Materials and Methods: Treatment was performed between June 2018 and June 2019 on 60 patients with androgenetic alopecia and associated telogen effluvium. Each patient underwent 4 sessions in total, each session was performed every 3 weeks. Global photography and trichoscopy were collected at every session of therapy. All patients filed out a self-assessment questionnaire. Results: Results were very promising, with improvement of hair density and thickening of the hair shaft diameter in most of patients seen with both global photography and trichoscopy. All patients were satisfied of the clinical result and reported a complete reduction in hair loss. No serious adverse side effects were reported. Conclusions: The use of growth factors associated with iontophoresis technique is a useful treatment for treating and preventing androgenetic alopecia. In addition, in case of associated telogen effluvium, this technique allows for an early stop of hair shedding, especially when cosmetic procedures do not provide satisfactory results in patients.

Download Full-text

IMPROVEMENT OF ALOPECIA AND DECREASES IN HAIR CORTISOL AND DIHYDROTESTOSTERONE CONCENTRATIONS AFTER THE TOPICAL USE OF PLANT EXTRACTS IN MEN WITH ANDROGENETIC ALOPECIA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2021.v14i8.41859 ◽

2021 ◽

pp. 71-79

Author(s):

SANG-HWAN DO ◽

RYUN-SUP AHN ◽

MYUNG-SUK NA ◽

HYUNG-RUEL JU

Keyword(s):

Plant Extracts ◽

Hair Shaft ◽

Androgenetic Alopecia ◽

Hair Cortisol ◽

Hair Density ◽

Main Effect ◽

Assessment Time ◽

Single Blind ◽

Single Time Point ◽

Time Point

Objectives: Plant extracts possessing specific constituents with anti-inflammatory, antimicrobial, antioxidant, or 5α-reductase inhibitory properties are known to provide benefits against androgenetic alopecia (AGA) in men. A solid shampoo was formulated, and it contained a mixture of six different plant extracts that possess these beneficial properties against AGA. The improvement in AGA and changes in steroid concentrations were assessed after 4 months of formulated shampoo use. Methods: This study was conducted based on a randomized, placebo-controlled, and single-blind design. Hair-related variables and hair and saliva samples were collected bi-monthly in the treatment (n=48) and placebo (n=52) groups and at a single time point in the hairy controls (n=50). Results: The formulated shampoo was more effective on AGA than the placebo based on the hair shaft thickness and hair density in the receding hairline. The baseline hair cortisol and dihydrotestosterone (DHT) concentrations were significantly higher in the treatment and placebo groups than in the hairy controls. After 4 months, the hair steroid concentrations in the treatment group were reduced to those observed in the hairy controls, although the main effect of time on hair steroid concentrations was negligible in the placebo group. Salivary cortisol and DHT levels during the post-awakening period were comparable among the groups or assessment time points. Conclusion: The constituents of plant extracts included in the formulated shampoo would prevent hair loss, increase hair growth effects, and reduce hair cortisol and DHT concentrations without changes in the post-awakening salivary steroid levels in men with AGA.

Download Full-text

Promoted Growth of Murine Hair Follicles by Controlled Release of Growth Factors from Biodegradable Hydrogel

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.288-289.133 ◽

2005 ◽

Vol 288-289 ◽

pp. 133-138

Author(s):

Makoto Ozeki ◽

Yasuhiko Tabata

Keyword(s):

Growth Factors ◽

Controlled Release ◽

Growth Factor ◽

Hair Follicle ◽

Skin Color ◽

Growth Cycle ◽

Hair Shaft ◽

Hair Follicles ◽

Follicle Growth ◽

Hair Follicle Growth

This study is an investigation to evaluate how the controlled release of different growth factors affects the hair follicle growth of mice in the second anagen stage of hair cycle. For the controlled release of basic fibroblast growth factor (bFGF) and hepatocyte growth factor (HGF), they were incorporating into biodegradable gelatin hydrogels, while a biodegradable collagen hydrogel was used for incorporation of vascular endothelial growth factor (VEGF). After subcutaneous implantation of the different hydrogels incorporating each growth factor or injection of phosphate buffered saline (PBS) containing the same dose of growth factor into the back of mice, the hair follicle growth was evaluated photometrically and histologically based on four parameters: the skin color of reverse side of the implanted or injected site, the number of vessels newly formed, the area occupied by hair follicle tissue, and the hair length. The area in close proximity to the implanted site of hydrogels incorporating growth factor was still dark in color 10 days after application. The hydrogel incorporating any type of growth factor enabled the hair follicles to increase the size, leading significantly enhanced area occupied by hair follicles per unit area of tissue. Implantation of the hydrogels incorporating growth factor increased significantly the number of blood vessels newly formed. Moreover, the length of hair shaft was elongated by the hydrogel incorporating growth factor to a significantly higher extent than the corresponding growth factor. Neither empty gelatin nor collagen hydrogels affected the hair follicle growth. These results indicate that the hydrogel incorporating growth factor induced the anagen-preservable activity. We conclude that the controlled release enabled growth factors to positively act on the hair growth cycle of mice, irrespective of the factor type.

Download Full-text

The Female Pattern Hair Loss: Review of Etiopathogenesis and Diagnosis

BioMed Research International ◽

10.1155/2014/767628 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 22

Author(s):

Anja Vujovic ◽

Véronique Del Marmol

Keyword(s):

Hair Loss ◽

Current Knowledge ◽

Diagnostic Testing ◽

Clinical Manifestations ◽

Hair Follicles ◽

Hair Density ◽

Female Pattern Hair Loss ◽

Significant Impairment ◽

Pattern Distribution

Female pattern hair loss (FPHL) is the most common hair loss disorder in women. Initial signs may develop during teenage years leading to a progressive hair loss with a characteristic pattern distribution. The condition is characterized by progressive replacement of terminal hair follicles over the frontal and vertex regions by miniaturized follicles, that leads progressively to a visible reduction in hair density. Women diagnosed with FPHL may undergo significant impairment of quality of life. FPHL diagnosis is mostly clinical. Depending on patient history and clinical evaluation, further diagnostic testing may be useful. The purpose of the paper is to review the current knowledge about epidemiology, pathogenesis, clinical manifestations, and diagnosis of FPHL.

Download Full-text

Morphological substantiation of clinical efficacy of platelet rich plasma in males with androgenic alopecia

Vestnik of Russian military medical Academy ◽

10.17816/brmma12245 ◽

2018 ◽

Vol 20 (3) ◽

pp. 67-71

Author(s):

E E Pakhomova ◽

I O Smirnova ◽

I N Telichko

Keyword(s):

Clinical Efficacy ◽

Growth Parameters ◽

Platelet Rich Plasma ◽

Hair Follicles ◽

Androgenetic Alopecia ◽

Sweat Glands ◽

Androgenic Alopecia ◽

Hair Density ◽

Plasma Therapy ◽

Four Levels

Morphological substantiation of the clinical efficacy of platelets rich plasma was carried out in the treatment of 22 men with androgenetic alopecia from the 1st to the 4th stage according to the Norwood-Hamilton scale were included. All patients received intradermal injections of platelets rich plasma 0,15 ml per injection. The course of treatment consisted of 4 procedures with an interval of 4 weeks. Clinical efficacy was assessed by the dynamics of morphometric indices of hair growth. Histological examination was carried out on horizontal sections, stained with hematoxylin and eosin, the morphology of the hair was counted at four levels. Evaluation of morphometric growth parameters conducted before treatment and 4 months after the onset of it. It was established that the therapy of platelet-rich plasma has a pronounced clinical efficacy, consisting in a significant (p=0,00025) increase in hair density by 11% and average hair diameter by 10% (p=0,00766), a 14% decrease in the share of hair follicles (p=0,00959). Histologically, the increase in hair density was significant at the level of the bulb of the hair follicles by 148% (p=0,0034) and at the level of the sweat glands by 65% (p=0,0326), and also by the tendency to increase their number at the level of the sebaceous glands. This was combined with a significant decrease in the proportion of telogen hair at 47% (p=0,0153). Thus, the positive clinical effect of plasma-rich plasma therapy in men suffering from androgenetic alopecia is based on reliable morphofunctional changes in the hair follicles.

Download Full-text

Quercitrin Stimulates Hair Growth with Enhanced Expression of Growth Factors via Activation of MAPK/CREB Signaling Pathway

Molecules ◽

10.3390/molecules25174004 ◽

2020 ◽

Vol 25 (17) ◽

pp. 4004

Author(s):

Jaeyoon Kim ◽

Soon Re Kim ◽

Yun-Ho Choi ◽

Jae young Shin ◽

Chang Deok Kim ◽

...

Keyword(s):

Growth Factors ◽

Energy Metabolism ◽

Signaling Pathway ◽

Hair Growth ◽

Hair Shaft ◽

Hair Follicles ◽

Major Constituent ◽

Dermal Papilla Cells ◽

Cellular Energy ◽

Cellular Energy Metabolism

The present study aimed to investigate the molecular mechanism of quercitrin, a major constituent of Hottuynia cordata extract, for its hair growth stimulating activities in cultured human dermal papilla cells (hDPCs). Quercitrin enhanced the cell viability and cellular energy metabolism in cultured hDPCs by stimulating the production of NAD(P)H and mitochondrial membrane potential (ΔΨ). The expression of Bcl2, an essential marker for anagen hair follicle and cell survival, was increased by quercitrin treatment. Quercitrin also increased the cell proliferation marker Ki67. The expression of growth factors—such as bFGF, KGF, PDGF-AA, and VEGF—were increased by quercitrin both in mRNA and protein levels. In addition, quercitrin was found to increase the phosphorylation of Akt, Erk, and CREB in cultured hDPCs, while inhibitors of MAPKs reversed the effects of quercitrin. Finally, quercitrin stimulated hair shaft growth in cultured human hair follicles. Our data obtained from present study are in line with those previously reported and demonstrate that quercitrin is (one of) the active compound(s) of Hottuynia cordata extract which showed hair growth promoting effects. It is strongly suggested that the hair growth stimulating activity of quercitrin was exerted by enhancing the cellular energy metabolism, increasing the production of growth factors via activation of MAPK/CREB signaling pathway.

Download Full-text