scholarly journals A Pseudo-Hyper Triiodothyroninemia Caused by Heterophile Antibodies Interference in Radio Immunoassay

Scimetr ◽  
2013 ◽  
Vol 2 (1) ◽  
Author(s):  
Majid Valizadeh ◽  
Fereidoun Azizi ◽  
Mehdi Hedayati
Keyword(s):  
Radio Immunoassay

INHIBITION OF LIPOLYTIC ACTIVITY OF SYNTHETIC β1–24 AND β1–39 CORTICOTROPHIN BY ANTI-ACTH ANTISERUM

1966 ◽  
Vol 51 (1) ◽  
pp. 88-94 ◽  
Author(s):  
A. Villanueva ◽  
S. J. H. Ashcroft ◽  
J. P. Felber
Keyword(s):  
Guinea Pig ◽  
Lipolytic Activity ◽  
Peptide Hormones ◽  
Fat Pad ◽  
Double Antibody ◽  
Epididymal Fat ◽  
Antibody Complex ◽  
Radio Immunoassay ◽  
Antigen Antibody

ABSTRACT The synthetic ACTH peptides β1–39 and β1–24 stimulated lipolysis as determined by the rat epididymal fat pad in vitro. The stimulating effect of these peptides was diminished by prior incubation of the peptides with antibodies produced by the guinea-pig against ACTH. The stimulating effect of these hormones was also diminished by the double antibody system used in the radio-immunoassay of ACTH and other peptide hormones, in which incubation with antiserum is followed by precipitation of the antigen-antibody complex by rabbit anti-guinea-pig-γ-globulin.

Prenatal diagnosis of Duchenne muscular dystrophy by radio-immunoassay of myoglobin in amniotic fluid

2008 ◽  
Vol 21 (5) ◽  
pp. 354-355 ◽  
Author(s):  
O. Török ◽  
K. Norregaard-Hansen ◽  
M. Szokol ◽  
K. Csécsei ◽  
A. Harsányi ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Duchenne Muscular Dystrophy ◽  
Muscular Dystrophy ◽  
Amniotic Fluid ◽  
Radio Immunoassay

FOLLICLE STIMULATING HORMONE (FSH) AND LUTEINIZING HORMONE-HUMAN CHORIONIC GONADOTROPIN (LH-HCG) CONCENTRATIONS IN PAIRED MATERNAL AND CORD SERA

PEDIATRICS ◽  
1974 ◽  
Vol 53 (1) ◽  
pp. 41-47
Author(s):  
Robert Penny ◽  
N. Olatunji Olambiwonnu ◽  
S. Douglas Frasier
Keyword(s):  
Luteinizing Hormone ◽  
Chorionic Gonadotropin ◽  
Follicle Stimulating Hormone ◽  
Poor Correlation ◽  
Male Fetus ◽  
Gonadotropin Secretion ◽  
Female Fetus ◽  
Cord Serum ◽  
Serum Gonadotropin ◽  
Radio Immunoassay

FSH and LH-HCG concentrations were determined by radio-immunoassay in paired maternal and cord sera. The sera of 50 mothers and 51 infants, 25 female and 26 male (one set of twins), were assayed. Mean (± SD) FSH concentration of mothers (3.4 ± 0.9 mIU/ml) giving birth to female infants was not different, p>0.1, from that of mothers (3.7 ± 0.7 mIU/ml) giving birth to male infants. In contrast, mean (± SD) LH-HCG concentration of mothers (15.99 ± 3.1 IU/ml) giving birth to female infants was significantly, p<0.005, greater than that of mothers (11.37 ± 5.0 IU/ml) giving birth to male infants. Cord serum FSH mean (± SD) concentration was significantly, p<0.005, greater in female infants (3.7 ± 0.5 mIU/ml) than male infants (2.4 ± 0.8 mIU/ml). However, mean (± SD) LH-HCG concentrations in female infants (0.122 ± 0.015 IU/ml) was significantly, p<0.005, less than that of male infants (0.156 ± 0.040 IU/ml). Poor correlation, on an individual basis and on a statistical basis, between maternal and cord serum FSH and LH-HCG concentrations was observed. The data of this investigation are consistent with fetal pituitary gonadotropin secretion, confirm previous observations that women bearing a female fetus have higher LH-HCG concentrations than those bearing a male fetus, and suggest a sex difference in cord serum gonadotropin concentrations.

scholarly journals The Adrenocortical Function Studies in Children

2017 ◽  
Vol 15 (3-4) ◽  
pp. 120
Author(s):  
Iwao Takakura
Keyword(s):  
Neonatal Period ◽  
Clinical Work ◽  
Adrenocortical Function ◽  
Chemical Structures ◽  
Pediatric Age Group ◽  
Loading Tests ◽  
Laboratory Procedures ◽  
Radio Immunoassay ◽  
Releasing Factors

The recent advances in the laboratory procedures make it possible to study endocrinological states in children more easily and more accurately even in the neonatal period. The successes in purification and the determination of chemical structures of various hormones and their releasing factors make it possible to carry out various loading tests. The progress in the measurement of various hormones and their metabolites especially in the field of radioimmunoassay is really remarkable.If we took adrenocortical function studies as an example, urinary 17 hydroxycorticosteroid was the only reliable item about ten years ago, then plasma 17-OHCS determination using Porter-Silver chromogen was introduced followed by plasma 11-OHCS determination with fluorescence spectrophotometry. Then, cortisol production rate determination became possible using radio-isotope dilution technique, and at present, plasma cortisol can be measured directly with protein competitive radio-immunoassay. Thus, the adrenocortical function studies in the pediatric age group has become easier and more reliable. The author likes to present some endocrinological results in children using various methods and now they can be applied in the clinical work.

Radio immunoassay employing gel filtration

1965 ◽  
Vol 12 (1) ◽  
pp. 163-172 ◽  
Author(s):  
E. Haber ◽  
L.B. Page ◽  
F.F. Richards
Keyword(s):  
Gel Filtration ◽  
Radio Immunoassay

Autoantibodies

2013 ◽  
pp. 473-532
Author(s):  
Gavin P Spickett
Keyword(s):  
Adrenal Cortex ◽  
Acetylcholine Receptor ◽  
Indirect Immunofluorescence ◽  
Direct Immunofluorescence ◽  
Auerbach’S Plexus ◽  
Radio Immunoassay ◽  
Receptor Antibodies ◽  
Auerbach's Plexus ◽  
Particle Agglutination

Introduction Techniques: overview Particle agglutination assays Immunoprecipitation assays Indirect immunofluorescence Direct immunofluorescence Radio-immunoassay (RIA) Enzyme-linked (EIA) and fluorescent immunoassays (FIA) Immunoblotting Acetylcholine-receptor antibodies (AChRAb) Actin antibodies Adrenal cortex autoantibodies Amphiphysin antibodies Anti-nuclear antibodies (ANA) and ANCA Aquaporin antibodies Auerbach’s plexus antibodies β‎2-GPI antibodies C1q antibodies...

Cardiopulmonary Dysfunction And Changes In Production Of TXA2 And PGI2 Induced By Intracoronary Injection With Arachidonic Acid

1981 ◽  
Author(s):  
I Isohisa ◽  
K Tanoue ◽  
S Ariga ◽  
C Sakakibara ◽  
H Yamazaki
Keyword(s):  
Blood Pressure ◽  
Specific Inhibitor ◽  
Ascending Aorta ◽  
Intracoronary Injection ◽  
St Depression ◽  
Propenoic Acid ◽  
Significant Difference ◽  
Radio Immunoassay ◽  
Wedge Pressure ◽  
Pressure Catheter

A 4 Wedge-Pressure catheter was inserted to 1 cm above aortic valve in ascending aorta through right common carotid artery of 16 rabbits. 1.5 mg/kg of sodium arachidonate (AA) was injected into the proximal portion of ascending aorta after inflation of balloon which obstructed the ascending aorta. Inflation remained for 5 sec, the solution was perfused into coronary arteries. ECG, respiratory movement and blood pressure were recorded. Before, 3 and 60 min after AA, 10 ml of heparinized blood was collected, added with 0.1 ml of Tris-HCl buffer (pH 8.0) containing indomethacin (10 μM in final concentration) and centrifuged to collect PPP for measurement of TXB2 and 6-Keto PGF1α using radio immunoassay. 8 rabbits were injected with 10 mg/kg of (E)-3-[4-(3- pyridylmethyl)phenyl]-2-methyl-2-propenoic acid (OKY-1580, a specific inhibitor on TXA2 synthetase) 3 min before AA injection. In control, arrhythmia and marked ST depression appeared in all cases after AA. Apnea was seen in 4 and 1 case died 7 min after AA. In OKY group, arrhythmia and apnea were not seen. 2 cases showed ST depression. Blood pressure decreased in both the groups similarly. TXB2 values were 1.091+0.498 ng/ml (mean+SD) before, 3.037±0.927 3 min after and 1.014±0.426 60 min after in control. In OKY group those were 1.3154±). 926, 0.830±0.334 and 0.764±0.343. There was a significant difference in the values 3 min after between both the groups. In control, 6-Keto PGF1α values were 3.337± 2.279 ng/ml before, 26.668±16.353 3 min after and 3.975± 3.548 60 min after. In OKY group, those were 2.151±0.528, 61.064±21.420 and 4.264±2.530. There was also a significant difference in the values 3 min after. Histologic findings of heart showed more remarkable ischemic changes in control than in OKY group. These findings suggest that the intracoronary injection with AA induced ischemic changes in heart through TXA2 synthesis at least partially and the changes were able to be prevented by OKY 1580.

Demonstration of Antithrombin III in Fresh and Cultured Endothelial Cells from Human Umbilical Cord

1979 ◽  
Author(s):  
Vivian Chan ◽  
T.K. Chan
Keyword(s):  
Endothelial Cells ◽  
Umbilical Cord ◽  
Antithrombin Iii ◽  
Cultured Cells ◽  
Active Role ◽  
Human Umbilical Cord ◽  
Thrombosis Research ◽  
Natural Inhibitor ◽  
Immunofluorescent Technique ◽  
Radio Immunoassay

We have shown by immunofluorescent technique that the distribution of antithrombin III (ATIII) in human tissues was concentrated around the microvasculature of the lungs and kidneys, as well as veins and small arteries of other organs (liver and spleen). It would seem that ATIII is stored and/or synthesized in the endothelial cells similar to Factor VIII-RAG and Plasminogen Activator. Endothelial cells were isolated from human umbilical cord by collagenase and cultured according to Chemethod described by Shearn etal (1977). In freshly isolated endothelial cells, ATIII could be demonstrated by indirect immunof1uorescent technique and radio immunoassay confirmed the presence of 14.8 ng per 106 cells. After 7 days’ culture, the supernatant from 106 cells contained about 15 ng and the cultured cells (106) contained 16.9 ng ATIII. The presence of ATIII in cultured cells was also confirmed by the positive immunofluorescence. Hence the endothelial cells play an active role in preventing thrombosis by the synthesis and liberation of ATIII, the major natural inhibitor of the intrinsic pathway of Coagulation.Reference: Shearn S.A., Peake I.R., Ciddings J.C., Humphrys J. and Bloom A.L. Thrombosis Research, 11, 43, 1977.

Plasma leptin concentration in pre- and post-weaning lambs

2003 ◽  
Vol 76 (2) ◽  
pp. 221-227 ◽  
Author(s):  
T. Tokuda ◽  
C. Delavaud ◽  
Y. Chilliard
Keyword(s):  
Plasma Concentrations ◽  
Metabolizable Energy ◽  
Blood Samples ◽  
Ruminant Species ◽  
Bean Meal ◽  
Radio Immunoassay ◽  
Metabolizable Energy Intake ◽  
Soya Bean Meal ◽  
Plasma Leptin ◽  
Daily Gain

AbstractLeptin has an important rôle in the control of appetite and energy expenditure. Several studies have reported the effects of leptin in ruminants. However, little is known about changes in circulating leptin concentrations in neonates of ruminant species, or the effects of weaning on plasma leptin concentrations. The main objectives of this experiment were: to examine plasma leptin concentrations in lambs, in the period from just after birth through to weaning; to examine the effect of weaning on plasma leptin concentrations, and to compare leptin concentrations measured using a ‘multi-species’ leptin radio-immunoassay (RIA) kit and a specific ovine RIA in lambs during the 6-month period after weaning. In a previous paper, we reported leptin concentrations using a commercial RIA during the post-weaning period. However, we were not able to measure plasma leptin concentrations from just after birth to weaning as they were apparently below the level of sensitivity of the assay. In the present study, five crossbred lambs were removed from their dams within 2 days after birth, and bottle-fed on milk replacer at a level sufficient to meet a 1•2 times maintenance metabolizable energy intake. Lambs were weaned 45 days after birth, and housed individually in pens. The lambs were offered timothy hay, rolled barley and soya-bean meal to meet a 200 g daily gain during the post-weaning period. During the pre-weaning period, blood samples were collected within 5 h of birth and thereafter at 09:00 h; every day from 2 to 6 days of age; at 2-day intervals from 6 to 14 days of age; and at 3-day intervals from 14 to 45 days of age. During the post-weaning period, blood samples were collected before and 3 and 6 h after the morning meal at the ages of 0•5, 1, 2, 3, 4, 5 and 6 months. Plasma leptin concentrations slightly increased (P < 0•05) just after birth and then remained constant until 45 days old (P > 0•05). Additionally, plasma leptin concentration was not significantly changed following weaning. During the post-weaning period, plasma leptin concentrations were compared using two RIA systems. The correlation between plasma leptin concentrations measured by the ‘multi-species’ leptin RIA kit and specific ovine RIA was poor (r = 0•41). These findings are consistent with other reports and suggest that the ‘multi-species’ leptin RIA kit is not suitable for estimating leptin plasma concentrations in ruminants.

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