scholarly journals Case Report: A Pediatric COVID-19 Patient with Pyelonephritis and Febrile Seizure as a Rare Clinical Manifestations

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Masoud Rezagholizamenjany ◽  
Parsa Yousefichaijan ◽  
Fakhreddin Shariatmadari ◽  
Manijeh Kahbazi ◽  
Vahid Falahati ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Febrile Seizure ◽  
Urine Analysis ◽  
Clinical Manifestations ◽  
Chain Reaction ◽  
Rare Presentation ◽  
Complete Evaluation ◽  
Polymerase Chain ◽  
Primary Evaluation ◽  
Case Based

: In this study, we report a rare presentation of COVID-19 virus, as febrile seizure. The patient was a 13-month-old girl with febrile seizure. Fever of the patient had started three days ago. Also, the patient had chills for five minutes in addition to cyanosis and tachycardia. On the primary evaluation, active urine analysis showed pyelonephritis. After a complete evaluation, the patient was diagnosed as a positive COVID-19 case based on polymerase chain reaction using nasopharyngeal and oropharyngeal swabs.

Using a Polymerase Chain Reaction as a Complementary Test to Improve the Detection of Dermatophyte Fungus in Nails

2014 ◽  
Vol 104 (3) ◽  
pp. 233-237 ◽  
Author(s):  
María José Iglesias Sánchez ◽  
Ana María Pérez Pico ◽  
Félix Marcos Tejedor ◽  
María Jesús Iglesias Sánchez ◽  
Raquel Mayordomo Acevedo
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequences ◽  
Classical Method ◽  
Clinical Manifestations ◽  
Culture Method ◽  
Clinical Samples ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Traditional Procedure ◽  
Polymerase Chain

Background Dermatomycoses are a group of pathologic abnormalities frequently seen in clinical practice, and their prevalence has increased in recent decades. Diagnostic confirmation of mycotic infection in nails is essential because there are several pathologic conditions with similar clinical manifestations. The classical method for confirming the presence of fungus in nail is microbiological culture and the identification of morphological structures by microscopy. Methods We devised a nested polymerase chain reaction (PCR) that amplifies specific DNA sequences of dermatophyte fungus that is notably faster than the 3 to 4 weeks that the traditional procedure takes. We compared this new technique and the conventional plate culture method in 225 nail samples. The results were subjected to statistical analysis. Results We found concordance in 78.2% of the samples analyzed by the two methods and increased sensitivity when simultaneously using the two methods to analyze clinical samples. Now we can confirm the presence of dermatophyte fungus in most of the positive samples in just 24 hours, and we have to wait for the result of culture only in negative PCR cases. Conclusions Although this PCR cannot, at present, substitute for the traditional culture method in the detection of dermatophyte infection of the nails, it can be used as a complementary technique because its main advantage lies in the significant reduction of time used for diagnosis, in addition to higher sensitivity.

Herpes simplex Virus Myelitis: Clinical Manifestations and Diagnosis by the Polymerase Chain Reaction Method

1998 ◽  
Vol 39 (3) ◽  
pp. 163-167 ◽  
Author(s):  
Hideto Nakajima ◽  
Daisuke Furutama ◽  
Fumiharu Kimura ◽  
Keiichi Shinoda ◽  
Nakaaki Ohsawa ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Clinical Manifestations ◽  
Polymerase Chain Reaction Method ◽  
Chain Reaction ◽  
Reaction Method ◽  
Polymerase Chain ◽  
Simplex Virus

scholarly journals Diagnostic Tools for Borrelia Assessment in Humans

2016 ◽  
Vol 10 (1) ◽  
pp. 62-69 ◽  
Author(s):  
Serena Bonin
Keyword(s):  
Polymerase Chain Reaction ◽  
Lyme Disease ◽  
Web Sites ◽  
Clinical Manifestations ◽  
Review Article ◽  
Diagnostic Tools ◽  
Chain Reaction ◽  
Wide Range ◽  
Controversial Topic ◽  
Polymerase Chain

Although the etiological agent of Lyme disease has been known since 1980s, diagnosis of Lyme disease is still a controversial topic because of the wide range of clinical manifestations and the limited diagnostic tools available to assessBorreliain humans.The most used diagnostic tool for Lyme disease is currently serology, but also Polymerase chain reaction (PCR) and other methods are often used to proveBorreliainfection in different patients’ specimens. The present article deals with most of the diagnostic tools used in clinical practice for Lyme disease detection in human samples. Direct and indirect specific methods forBorreliainfection detection will be discussed.The most recent peer reviewed publications as well as original results from our study and information provided by companies’ web sites have been analyzed to compile this review article.

scholarly journals Clinical Manifestations of Bartonella henselae Infection Among Children

2021 ◽  
Vol 41 (1) ◽  
pp. 8-14
Author(s):  
Ivana Valenčak-Ignjatić ◽  
Diana Didović ◽  
Branko Miše ◽  
Marija Gužvinec ◽  
Oktavija Đaković Rode ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bartonella Henselae ◽  
Acute Infection ◽  
Clinical Manifestations ◽  
Unknown Origin ◽  
Immunofluorescence Assay ◽  
University Hospital ◽  
Chain Reaction ◽  
Mild Disease ◽  
Polymerase Chain

Objectives: The aim of this study was to analyze clinical manifestations, epidemiology and laboratory parameters of B. henselae infection among children treated at the University Hospital for Infectious Diseases “Dr. Fran Mihaljević”, Zagreb from January 2014 until June 2019. Materials and methods: We retrospectively analyzed the epidemiology, clinical and laboratory characteristics among children with positive indirect immunofluorescence assay for B. henselae IgM and IgG or positive B. henselae polymerase chain reaction from lymph node aspirate. Results: A total of 104 patients, 47 (45,1%) female and 57 (54,8%) male were enrolled. The median age was 9,7 (range, 1,1 to 17,3 years). A history of cat contact was present in 101 (97,1%) children. Acute infection was serologically confirmed in 87 (83,6%), in 5 (4,8%) with PCR while both methods were positive in 12 (11,5%) patients. The presentation on B. henselae infection were regional lymphadenopathy , disseminated disease, encephalopathy and fever of unknown origin. Suppurative inflammation was the most common complication in patients with lymphadenopathy 12/92 (13%). Full recovery was the most frequent outcome (96,1%). Conclusion lesion: B. henselae infection among children is usually a mild disease presented as regional lymphadenopathy. Serology and polymerase chain reaction are useful tests for diagnosis. Treatment duration and choice of therapy depend on clinical manifestation and developed complications.

Actinobacillus actinomycetemcomitansgenetic heterogeneity: amplification of JP2-likeltxpromoter pattern correlated with specific arbitrarily primed polymerase chain reaction (AP-PCR) genotypes from human but not marmoset Brazilian isolates

2002 ◽  
Vol 48 (7) ◽  
pp. 602-610 ◽  
Author(s):  
L Saddi-Ortega ◽  
M A.R Carvalho ◽  
P S Cisalpino ◽  
E S.A Moreira
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Manifestations ◽  
Pcr Amplification ◽  
Actinobacillus Actinomycetemcomitans ◽  
Periodontal Pathogen ◽  
Chain Reaction ◽  
Arbitrary Primers ◽  
Close Relationship ◽  
Host Origin ◽  
Polymerase Chain

Specific clonal types of Actinobacillus actinomycetemcomitans, a major human periodontal pathogen, may be responsible for clinical manifestations and the production of leukotoxin virulence factors. Leukotoxicity is associated with genetic polymorphism at the promoter region of the leukotoxin (ltx) gene. Here, we describe the use of arbitrarily primed polymerase chain reaction (AP-PCR) and ltx promoter PCR to molecularly characterise 35 A. actinomycetemcomitans Brazilian isolates: 21 of human origin and 14 from captive marmosets (Callitrix spp., primates commonly used as animal models for periodontal research). The discriminative capacity of each of 12 arbitrary primers was found to be variable, yielding between 3 and 24 PCR amplitypes. Combination of the results for all primers led to characterisation of 14 genotypes that grouped into four major clusters based on genetic similarity. Clusters 2, 3, and 4 were discriminative to host origin. A correlation with periodontal disease was suggested for strains belonging to clusters 3 and 4. The JP2-like PCR amplification pattern, associated with highly leukotoxic strains, was exclusive to human isolates and present in 29% of human isolates where it occurred in close relationship with AP genotypes L and J (cluster 3).Key words: Actinobacillus actinomycetemcomitans, marmosets, AP-PCR, leukotoxin.

scholarly journals Microscopy and Polymerase Chain Reaction Detection ofLeishmania chagasiin the Pleural and Ascitic Fluid of a Patient with AIDS: Case Report and Review of Diagnosis and Therapy of Visceral Leishmaniasis

2004 ◽  
Vol 15 (4) ◽  
pp. 231-234 ◽  
Author(s):  
Ada RS Diehl ◽  
Rodrigo P dos Santos ◽  
Ricardo Zimmerman ◽  
Letícia P Luz ◽  
Tanara Weiss ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Visceral Leishmaniasis ◽  
Ascitic Fluid ◽  
Clinical Manifestations ◽  
Histological Evaluation ◽  
Diagnostic Tools ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Brazilian Patient ◽  
Polymerase Chain

Atypical visceral leishmaniasis is increasingly reported in immunocompromised patients, including patients with AIDS. A case of visceral leishmaniasis in an HIV-infected Brazilian patient with pulmonary and peritoneal involvement is reported. Histological evaluation of pleural fluid and ascites aspirate revealed macrophages with intracellularLeishmania. Polymerase chain reaction analysis was positive forLeishmaniain the pleural and ascitic fluid with use of primers specific forLeishmania chagasi. In addition to classical methods for diagnosing leishmaniasis, such as microscopy and culture, polymerase chain reaction detection and identification ofLeishmaniaspecies in pleural effusions and ascites are important diagnostic tools that should be considered by clinicians evaluating HIV-infected patients from endemic areas of visceral leishmaniasis. The authors review the clinical manifestations, diagnostic and therapeutic aspects of visceral leishmaniasis in immunocompetent and HIV-infected patients.

scholarly journals Polymerase Chain Reaction Detection of Lyme Disease:Correlation With Clinical Manifestations and Serologic Responses

1996 ◽  
Vol 105 (5) ◽  
pp. 647-654 ◽  
Author(s):  
C. Lars Mouritsen ◽  
Carl T. Wittwer ◽  
Christine M. Litwin ◽  
Liming Yang ◽  
Janis J. Weis ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Manifestations ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Deteksi transmisi virus dengue pada nyamuk wild Aedes Aegypti betina di Kota Manado

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Grace Trovancia ◽  
Angle Sorisi ◽  
Josef S.B. Tuda
Keyword(s):  
Polymerase Chain Reaction ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Reverse Transcription ◽  
Clinical Manifestations ◽  
Virus Transmission ◽  
Survey Study ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract: Dengue hemorrhagic fever is an acute disease with clinical manifestations of hemorrhage caused by dengue virus infection. Manado is endemic dengue. Dengue virus has the ability to maintain its existence in nature through horizontal and vertical transmission. There are several ways to detect the dengue virus by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and immunohistochemistry Streptavidin Biotin Peroxidase Complex (ISBPC). This research aims to determine the wild Aedes aegypti population in Manado and to detect dengue virus in wild mosquito Aedes aegypti by ISBPC methods. This was a descriptive survey study with a cross sectional design to describe the transmission of dengue virus in wild mosquito Aedes aegypti in the city of Manado. The results showed that there were 5 wild Aedes aegypti mosquitoes positive for dengue virus, and 36 wild Aedes aegypti mosquitoes negative containing dengue virus. Conclusion: Of the 41 samples immunohistochemistry tested, 5 samples showed dengue virus transmission in wild mosquito Aedes aegypti in Manado which is a positive possibility of horizontal transmission.Keywords: detection of dengue virus, transmission, wild Aedes aegypti, Manado. Abstrak: Demam berdarah dengue adalah suatu penyakit akut dengan manifestasi klinis perdarahan yang disebabkan oleh infeksi virus dengue. Manado merupakan daerah endemis demam berdarah. Virus dengue memiliki kemampuan untuk mempertahankan keberadaannya di alam melalui transmisi horizontal dan vertikal. Ada beberapa cara untuk mendeteksi virus dengue yaitu Reverse Transcription-Polymerase Chain Reaction (RT-PCR) dan imunohistokimia Streptavidin Biotin Peroxidase Complex (SBPC). Penelitian ini bertujuan untuk mengetahui populasi nyamuk wild Aedes aegypti di Kota Manado dan mendeteksi virus dengue pada nyamuk wild Aedes aegypti dewasa menggunakan metode imunohistokimia streptavidin biotin peroxidase complex (ISBPC). Jenis penelitian ialah survei deskriptif dengan desain potong lintang untuk mengetahui gambaran transmisi virus dengue pada nyamuk wild Aedes aegypti betina di Kota Manado. Hasil pene;itian mendapatkan 5 nyamuk wild Aedes aegypti positif mengandung virus dengue, dan 36 nyamuk wild Aedes aegypti negatif mengandung virus dengue. Simpulan: Berdasarkan hasil penelitian dapat disimpulkan bahwa dari 41 sampel yang telah diuji imunohistokimia, 5 sampel gambaran transmisi virus dengue pada nyamuk wild Aedes aegypti betina di Kota Manado yang kemungkinan transmisi horizontal adalah positif. Kata kunci: deteksi virus dengue, transmisi, wild Aedes aegypti, Manado.

scholarly journals Coronavirus disease 19 with gastrointestinal symptoms as initial manifestations: a case report

2020 ◽  
Vol 48 (9) ◽  
pp. 030006052095225
Author(s):  
Chun-Yang Wu ◽  
Xiao-Pin Yu ◽  
Ada Hoi Yan Ma ◽  
Li-Ping Wang ◽  
Nai-Bin Yang ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Severe Acute Respiratory Syndrome ◽  
Clinical Manifestations ◽  
Gastrointestinal Symptoms ◽  
Lower Lobe ◽  
Ground Glass Opacity ◽  
Chain Reaction ◽  
Chronic Superficial Gastritis ◽  
Polymerase Chain ◽  
The Right

Since the outbreak of coronavirus disease 2019 (COVID-19) in December 2019, an epidemic has spread rapidly worldwide. COVID-19 is caused by the highly infectious severe acute respiratory syndrome coronavirus-2. A 42-year-old woman presented to hospital who was suffering from epigastric discomfort and dyspepsia for the past 5 days. Before the onset of symptoms, she was healthy, and had no travel history to Wuhan or contact with laboratory-confirmed COVID-19 cases. An examination showed chronic superficial gastritis with erosion and esophagitis. Enhanced magnetic resonance imaging of the abdomen showed a lesion in the right lower lobe of the lungs. Chest computed tomography showed multiple ground-glass opacity in the lungs. Reverse transcription-polymerase chain reaction was negative for severe acute respiratory syndrome coronavirus-2. There was no improvement after antibiotic treatment. Polymerase chain reaction performed 2 days later was positive and she was diagnosed with COVID-19. After several days of antiviral and symptomatic treatments, her symptoms improved and she was discharged. None of the medical staff were infected. Clinical manifestations of COVID-19 are nonspecific, making differentiating it from other diseases difficult. This case shows the sequence in which symptoms developed in a patient with COVID-19 with gastrointestinal symptoms as initial manifestations.

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